##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545550_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1052785
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.346896089894898	31.0	31.0	33.0	30.0	34.0
2	31.694478929695997	31.0	31.0	34.0	30.0	34.0
3	31.791406602487687	31.0	31.0	34.0	30.0	34.0
4	35.48863918083939	37.0	35.0	37.0	33.0	37.0
5	35.35463271228218	37.0	35.0	37.0	33.0	37.0
6	35.445981848145635	37.0	35.0	37.0	33.0	37.0
7	35.87036004502344	37.0	35.0	37.0	35.0	37.0
8	35.89710339717986	37.0	35.0	37.0	35.0	37.0
9	37.60386213709352	39.0	37.0	39.0	35.0	39.0
10	36.977645958101604	39.0	37.0	39.0	32.0	39.0
11	36.57914293991651	39.0	35.0	39.0	32.0	39.0
12	35.55728852519745	37.0	35.0	39.0	31.0	39.0
13	35.07499821900958	37.0	35.0	39.0	30.0	39.0
14	36.01555018356075	38.0	35.0	40.0	30.0	41.0
15	36.33861804641973	38.0	35.0	40.0	31.0	41.0
16	36.56218506152728	38.0	35.0	40.0	32.0	41.0
17	36.53699568287922	38.0	35.0	40.0	32.0	41.0
18	36.51483731246171	37.0	35.0	40.0	31.0	41.0
19	36.43463575183917	37.0	35.0	40.0	31.0	41.0
20	36.26106564968156	37.0	35.0	40.0	31.0	41.0
21	36.05171521250778	36.0	34.0	40.0	31.0	41.0
22	35.946532292918306	36.0	34.0	40.0	30.0	41.0
23	35.94621503915804	36.0	34.0	40.0	31.0	41.0
24	35.88286402256871	36.0	34.0	40.0	31.0	41.0
25	35.76874290572149	36.0	34.0	40.0	30.0	41.0
26	35.667754574770726	36.0	34.0	40.0	30.0	41.0
27	35.60360282488827	36.0	34.0	40.0	30.0	41.0
28	35.628046562213555	36.0	34.0	40.0	30.0	41.0
29	35.63436884074146	36.0	34.0	40.0	30.0	41.0
30	35.58944608823264	36.0	34.0	40.0	30.0	41.0
31	35.3854946641527	36.0	34.0	40.0	29.0	41.0
32	35.19561543904976	35.0	34.0	40.0	29.0	41.0
33	35.0183836205873	35.0	34.0	40.0	28.0	41.0
34	34.869151821122074	35.0	34.0	40.0	27.0	41.0
35	34.7345317419986	35.0	34.0	40.0	27.0	41.0
36	34.51507287812801	35.0	33.0	40.0	25.0	41.0
37	34.44219000080738	35.0	33.0	40.0	25.0	41.0
38	34.45623085435298	35.0	33.0	40.0	26.0	41.0
39	34.424599514620745	35.0	33.0	40.0	25.0	41.0
40	34.21816515242903	35.0	33.0	40.0	24.0	41.0
41	34.23107282113632	35.0	33.0	40.0	24.0	41.0
42	34.15401055296191	35.0	33.0	40.0	24.0	41.0
43	34.08903337338583	35.0	33.0	40.0	24.0	41.0
44	34.0358895690953	35.0	33.0	40.0	24.0	41.0
45	33.99345735359071	35.0	33.0	40.0	24.0	41.0
46	33.98024097987718	35.0	33.0	39.0	24.0	41.0
47	33.938101321732354	35.0	33.0	39.0	24.0	41.0
48	33.9195372274491	35.0	33.0	39.0	24.0	41.0
49	33.913194051966926	35.0	33.0	39.0	24.0	41.0
50	33.756134443404875	35.0	33.0	39.0	24.0	41.0
51	33.592782002023206	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	0.0
13	3.0
14	4.0
15	27.0
16	115.0
17	259.0
18	582.0
19	1152.0
20	1989.0
21	3106.0
22	4690.0
23	6635.0
24	9705.0
25	13462.0
26	17726.0
27	20758.0
28	22749.0
29	25963.0
30	31102.0
31	37780.0
32	47117.0
33	61561.0
34	108564.0
35	175883.0
36	69444.0
37	82916.0
38	114481.0
39	194916.0
40	95.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.264246736038224	23.97241602036503	28.284977464534546	14.478359779062203
2	30.886363312547193	26.352864070061788	29.75840271280461	13.002369904586406
3	29.252126502562252	24.895776440583788	31.575772831109866	14.276324225744098
4	25.825785891706282	27.64391589925768	31.15916355191231	15.371134657123726
5	23.25641037818738	32.79216554187227	29.870581362766373	14.080842717173972
6	20.293507221322493	42.40751910409058	25.96266094216768	11.33631273241925
7	86.94310804200288	4.7989855478564	6.537707129185922	1.7201992809548008
8	88.43866506456683	3.4841871797185555	6.1520633367686655	1.9250844189459386
9	84.48752594309379	4.87658923711869	7.84367178483736	2.792213034950156
10	53.228721913781065	22.15552083283861	13.828844445922005	10.786912807458313
11	47.29883119535327	19.55593972178555	19.87034389737696	13.274885185484218
12	43.824617561990344	19.57959127457173	24.003856437924174	12.591934725513756
13	20.94815180687415	43.35880545410507	22.217071861776148	13.475970877244642
14	14.662063004317123	44.87611430633985	25.91906229667026	14.542760392672768
15	13.594893544265924	22.272733749056076	49.61696832686636	14.515404379811642
16	14.98321119696804	17.223649653063067	48.38319314959845	19.409946000370446
17	14.60003704460075	18.42683928817375	28.95890423970706	38.01421942751844
18	20.21419378125638	22.711094857924458	37.241886994970486	19.83282436584868
19	29.878275241383566	24.195253541796284	24.992757305622707	20.933713911197444
20	31.72357128948456	22.12892470922363	26.228242233694438	19.919261767597373
21	21.33512540547215	29.072412695849582	28.456332489539648	21.13612940913862
22	22.2496521132045	24.24056193809752	24.469573559653682	29.040212389044296
23	17.64215865537598	31.218814857734483	24.81028890039277	26.32873758649677
24	18.31950493215614	22.627507040848798	42.41872747047118	16.634260556523888
25	16.57992847542471	25.26194807106864	37.65764139876613	20.50048205474052
26	16.420826664513648	35.22894038193933	26.466467512360076	21.88376544118695
27	16.76078211600659	36.937171407267385	27.766827984821212	18.535218491904804
28	14.1365046044539	28.91730030348077	38.53882796582398	18.40736712624135
29	14.367605921436951	24.131897775899162	38.42199499422959	23.078501308434298
30	17.10539188913216	33.48556447897719	31.003196284141588	18.405847347749067
31	28.456522461851186	28.935917590011258	24.24930066442816	18.3582592837094
32	29.60319533428003	26.318859026296913	26.128506770138255	17.9494388692848
33	28.66454214298266	28.866767668612297	23.261919575221913	19.206770613183128
34	18.07425067796369	27.287337870505375	27.44881433531063	27.18959711622031
35	19.392753506176476	25.58756061304065	32.61292666593844	22.40675921484444
36	30.914954145433306	25.323594086161943	25.420289992733558	18.341161775671196
37	19.36387771482306	35.08589123135303	27.690839060206972	17.85939199361693
38	19.248469535565192	34.289052370616986	24.34371690326135	22.118761190556476
39	20.314879106370245	33.37044125818662	26.882791833090323	19.431887802352808
40	25.134476650028258	26.38050504139022	25.170761361531557	23.314256947049966
41	16.658956957023513	25.21084551926557	27.922985224903467	30.207212298807452
42	20.55756873435697	24.842774165665354	25.126497812943764	29.473159287033912
43	21.057100927539814	25.446696144037006	26.501707376149923	26.994495552273257
44	18.380011113380224	29.585242950839913	29.885494189221923	22.149251746557937
45	16.30095413593469	40.010163518667156	22.057685092397783	21.631197253000376
46	20.860859529723545	36.36972411271057	24.836695051696214	17.93272130586967
47	20.1961464116605	28.860593568487392	26.692534563087428	24.250725456764677
48	21.599851821597003	25.100661578574922	32.514805967030306	20.78468063279777
49	21.01540200515775	24.215105648351752	33.520044453520896	21.2494478929696
50	18.897875634626253	35.30074991569979	26.172960291037583	19.628414158636378
51	17.582507349553804	35.54296461290767	24.13636212522025	22.73816591231828
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	859.0
1	1399.0
2	1939.0
3	4647.0
4	7355.0
5	5572.0
6	3789.0
7	4055.5
8	4322.0
9	4758.0
10	5194.0
11	5279.5
12	5365.0
13	5180.0
14	4995.0
15	4902.5
16	4810.0
17	4495.0
18	4180.0
19	4203.0
20	4226.0
21	4302.0
22	4378.0
23	4277.5
24	4177.0
25	4720.5
26	7332.0
27	9400.0
28	10942.5
29	12485.0
30	14144.5
31	15804.0
32	18366.5
33	20929.0
34	26281.5
35	31634.0
36	33990.5
37	36347.0
38	41623.0
39	46899.0
40	62667.0
41	78435.0
42	96967.5
43	115500.0
44	112088.5
45	108677.0
46	103785.0
47	98893.0
48	91832.0
49	84771.0
50	78062.0
51	71353.0
52	63924.5
53	56496.0
54	50727.0
55	44958.0
56	40644.5
57	36331.0
58	33443.0
59	30555.0
60	28064.0
61	25573.0
62	22578.5
63	19584.0
64	17481.0
65	15378.0
66	13029.0
67	10680.0
68	8933.5
69	7187.0
70	6333.0
71	5479.0
72	4460.0
73	3441.0
74	2900.0
75	1830.0
76	1301.0
77	1080.5
78	860.0
79	581.0
80	302.0
81	241.5
82	181.0
83	119.0
84	57.0
85	63.5
86	70.0
87	36.5
88	3.0
89	4.5
90	6.0
91	4.0
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1052785.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.73461041083163
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.06882482172504	13.399205894446938
2	6.845921050599816	2.291276433701981
3	2.367653559772554	1.1886527973183703
4	1.1532359459243335	0.7719581706724243
5	0.6961809787478219	0.5825158727388126
6	0.4845017171152912	0.48647684875820113
7	0.3876196189427016	0.45406643174207856
8	0.283861919664637	0.3800254908846788
9	0.257488114803301	0.38780669579874577
>10	4.094992611715859	17.347635855167386
>50	1.8194437654325923	21.873034298967948
>100	1.5357087320406884	39.56479066963501
>500	0.002283581757681321	0.21495853719044447
>1k	0.002283581757681321	1.0575960029769869
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4097	0.3891582801806637	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3910	0.37139586905208566	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1764	0.16755557877439362	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1299	0.12338701634236811	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.026691109770750912	0.0
2	0.0	0.0	0.0	0.09992543586772228	0.0
3	0.0	0.0	0.0	0.14542380448049697	0.0
4	0.0	0.0	0.0	0.22492721685814293	0.0
5	0.0	0.0	0.0	0.38374406930189925	0.0
6	0.0	0.0	0.0	0.519479285894081	0.0
7	0.0	0.0	0.0	0.5959431412871574	0.0
8	0.0	0.0	0.0	0.7778416295824884	0.0
9	0.0	0.0	0.0	0.8348333230431665	0.0
10	0.0	0.0	0.0	0.9420726929050091	0.0
11	0.0	0.0	0.0	1.0823672449740451	0.0
12	0.0	0.0	0.0	1.187136974785925	0.0
13	0.0	0.0	0.0	1.225891326339186	0.0
14	0.0	0.0	0.0	1.2410891112620335	0.0
15	0.0	0.0	0.0	1.2705348195500505	0.0
16	0.0	0.0	0.0	1.3412045194412914	0.0
17	0.0	0.0	0.0	1.4227026410900612	0.0
18	0.0	0.0	0.0	1.542005252734414	0.0
19	0.0	0.0	0.0	1.5979520984816462	0.0
20	0.0	0.0	0.0	1.6505744287770057	0.0
21	0.0	0.0	0.0	1.7349221350988093	0.0
22	0.0	0.0	0.0	1.814615519787991	0.0
23	0.0	0.0	0.0	1.919765194222942	0.0
24	0.0	0.0	0.0	1.9985087173544456	0.0
25	0.0	0.0	0.0	2.060059746291978	0.0
26	0.0	0.0	0.0	2.1207558998276	0.0
27	1.8997231153559369E-4	0.0	0.0	2.176417787107529	0.0
28	1.8997231153559369E-4	0.0	0.0	2.245282750039182	0.0
29	1.8997231153559369E-4	0.0	0.0	2.313387823724692	0.0
30	1.8997231153559369E-4	0.0	0.0	2.411223564165523	0.0
31	1.8997231153559369E-4	0.0	0.0	2.4978509382257537	0.0
32	1.8997231153559369E-4	0.0	0.0	2.575359641332276	0.0
33	1.8997231153559369E-4	0.0	0.0	2.648783939740783	0.0
34	1.8997231153559369E-4	0.0	0.0	2.7280023936511255	0.0
35	1.8997231153559369E-4	0.0	0.0	2.8484448391646917	0.0
36	1.8997231153559369E-4	0.0	0.0	2.9345972824460835	0.0
37	1.8997231153559369E-4	0.0	0.0	3.0237892827120447	0.0
38	1.8997231153559369E-4	0.0	0.0	3.109751753681901	0.0
39	1.8997231153559369E-4	0.0	0.0	3.198088878545952	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGCG	40	6.8139343E-9	45.000004	1
ACTATCG	20	7.0330146E-4	45.000004	7
TGCCCCG	20	7.0330146E-4	45.000004	22
GTCGATC	20	7.0330146E-4	45.000004	9
GGCGTAT	20	7.0330146E-4	45.000004	8
TAAGTCG	40	6.8139343E-9	45.000004	1
TAACGTG	20	7.0330146E-4	45.000004	17
TCGATTG	45	3.8562575E-10	45.000004	1
CGTTCAC	45	3.8562575E-10	45.000004	21
TCGGCGA	20	7.0330146E-4	45.000004	11
CCCGTTC	20	7.0330146E-4	45.000004	32
GCCGAAT	20	7.0330146E-4	45.000004	41
CTACGAT	20	7.0330146E-4	45.000004	19
TATTCGA	20	7.0330146E-4	45.000004	35
TCGTAAT	20	7.0330146E-4	45.000004	43
TCATCGG	20	7.0330146E-4	45.000004	2
CGTAATC	20	7.0330146E-4	45.000004	44
TTACGAG	40	6.8139343E-9	45.000004	1
ATGCATC	20	7.0330146E-4	45.000004	36
CCCGCAT	20	7.0330146E-4	45.000004	45
>>END_MODULE