FastQCFastQC Report
Thu 26 May 2016
SRR1545549_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545549_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1393330
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52860.37937889803564123No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC35680.25607716764872646No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG16440.11799071289644233No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG15800.11339740047224993No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATAT1450.045.0000049
ATGCGAG600.045.0000041
TATCCGG302.1658343E-645.0000042
TAGACGT351.2121745E-745.022
CGTTACA207.0338027E-445.035
ACCTAGT207.0338027E-445.09
TACGATA351.2121745E-745.042
CTACGAT351.2121745E-745.041
TGACACG502.1827873E-1145.01
TCTAACG502.1827873E-1145.01
ACTACGT207.0338027E-445.017
TCGTCGG253.8912334E-545.033
GAACGTA253.8912334E-545.020
TCGTAAG207.0338027E-445.01
TTACGTA253.8912334E-545.021
AACCTAG253.8912334E-545.031
ACCGTTG207.0338027E-445.01
ACGTATG453.8562575E-1045.01
TCGAGTA253.8912334E-545.026
CACAACG207.0338027E-445.012