##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545549_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1393330
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.29716793580846	31.0	31.0	33.0	30.0	34.0
2	31.636671140361578	31.0	31.0	34.0	30.0	34.0
3	31.736785973172186	31.0	31.0	34.0	30.0	34.0
4	35.45657812578499	37.0	35.0	37.0	33.0	37.0
5	35.302607422505794	37.0	35.0	37.0	33.0	37.0
6	35.37741669238443	37.0	35.0	37.0	33.0	37.0
7	35.823840009186625	37.0	35.0	37.0	35.0	37.0
8	35.85156710901222	37.0	35.0	37.0	35.0	37.0
9	37.52752398929184	39.0	37.0	39.0	35.0	39.0
10	36.91419835932622	39.0	37.0	39.0	32.0	39.0
11	36.52916753389362	39.0	35.0	39.0	32.0	39.0
12	35.645912310795005	37.0	35.0	39.0	31.0	39.0
13	35.23679889186338	37.0	35.0	39.0	30.0	39.0
14	36.16549991746392	38.0	35.0	40.0	30.0	41.0
15	36.446589106672505	38.0	35.0	40.0	31.0	41.0
16	36.633168021933066	38.0	35.0	40.0	32.0	41.0
17	36.58157148701313	38.0	35.0	40.0	31.0	41.0
18	36.50930648159445	38.0	35.0	40.0	31.0	41.0
19	36.46606690446628	37.0	35.0	40.0	31.0	41.0
20	36.28873059504927	37.0	35.0	40.0	31.0	41.0
21	36.10420288086814	37.0	35.0	40.0	30.0	41.0
22	36.01739932392183	37.0	34.0	40.0	30.0	41.0
23	35.96060660432202	37.0	34.0	40.0	30.0	41.0
24	35.898268177675064	37.0	34.0	40.0	30.0	41.0
25	35.76152383139672	36.0	34.0	40.0	30.0	41.0
26	35.662596082765745	36.0	34.0	40.0	30.0	41.0
27	35.595548075473864	36.0	34.0	40.0	30.0	41.0
28	35.607979444926904	36.0	34.0	40.0	30.0	41.0
29	35.60229522080196	36.0	34.0	40.0	30.0	41.0
30	35.54289149017103	36.0	34.0	40.0	30.0	41.0
31	35.359846554656826	36.0	34.0	40.0	29.0	41.0
32	35.16623484745179	36.0	34.0	40.0	29.0	41.0
33	35.015099796889466	36.0	34.0	40.0	27.0	41.0
34	34.8688185856904	36.0	34.0	40.0	27.0	41.0
35	34.72866657575736	36.0	34.0	40.0	26.0	41.0
36	34.546128340019955	35.0	33.0	40.0	25.0	41.0
37	34.463240581915265	35.0	33.0	40.0	25.0	41.0
38	34.441329763946804	35.0	33.0	40.0	25.0	41.0
39	34.390313134720415	35.0	33.0	40.0	24.0	41.0
40	34.213678023153165	35.0	33.0	40.0	24.0	41.0
41	34.196383484171015	35.0	33.0	40.0	24.0	41.0
42	34.13265773363094	35.0	33.0	40.0	24.0	41.0
43	34.05703889243754	35.0	33.0	40.0	24.0	41.0
44	33.99523156753963	35.0	33.0	40.0	23.0	41.0
45	33.94033287160974	35.0	33.0	40.0	23.0	41.0
46	33.89510094521757	35.0	33.0	39.0	23.0	41.0
47	33.85716520852921	35.0	33.0	39.0	23.0	41.0
48	33.81961487946143	35.0	33.0	39.0	23.0	41.0
49	33.76476929370644	35.0	33.0	39.0	24.0	41.0
50	33.615833291467204	35.0	33.0	39.0	24.0	41.0
51	33.44033574243001	35.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	1.0
11	3.0
12	1.0
13	8.0
14	13.0
15	45.0
16	158.0
17	398.0
18	891.0
19	1734.0
20	2901.0
21	4487.0
22	6709.0
23	9191.0
24	12695.0
25	17779.0
26	23597.0
27	28098.0
28	31581.0
29	36088.0
30	41832.0
31	51424.0
32	63191.0
33	81054.0
34	138529.0
35	211179.0
36	98524.0
37	115922.0
38	159484.0
39	255645.0
40	166.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.22855317835688	23.697688271981512	26.982552589838733	14.091205959822869
2	32.17816310565336	24.706709824664653	29.332318976839655	13.782808092842327
3	29.263491060983398	25.607286141832873	31.269907344275943	13.859315452907783
4	26.505063409242606	27.51107060064737	30.401699525596953	15.582166464513072
5	23.847186237287648	32.10467010686629	29.627152218067508	14.42099143777856
6	21.284835609654568	41.88921504596901	26.456403005748818	10.369546338627604
7	85.93211945483124	4.913050031220171	7.157959708037579	1.9968708059110192
8	87.12451465194893	3.9534065870970982	6.8009014375632475	2.1211773233907256
9	82.77845162309	5.688171502802638	8.554900849045094	2.978476025062261
10	51.23438094349508	22.23342639575693	14.139220428757007	12.392972231990985
11	44.47072839887177	20.920384976997553	19.463802544982165	15.145084079148516
12	40.79148514709365	20.05289486338484	25.24527570640121	13.910344283120294
13	22.168330546245326	38.736552001320575	24.213431132610367	14.88168631982373
14	16.284512642374743	40.420288086813606	27.647649874760464	15.647549396051186
15	15.951066868581027	23.014433048882893	46.19465596807648	14.839844114459606
16	17.071260936030946	18.159660669044662	45.15549080260958	19.61358759231481
17	17.91535386448293	18.11121557707076	29.76566929585956	34.20776126258675
18	21.535529989306195	22.769910932801274	34.81666224081876	20.877896837073774
19	29.748587915282094	23.011203376084634	25.98257412099072	21.257634587642553
20	30.850552274048503	22.559766889394474	25.931329979258322	20.6583508572987
21	22.909863420725888	26.00417704348575	29.41995076543245	21.66600877035591
22	23.799458850380027	23.320031866104944	24.988624374699462	27.891884908815573
23	20.194856925495035	29.30949595573195	25.370658781480337	25.124988337292674
24	19.929880215024436	22.89830836915878	39.185261208758874	17.98655020705791
25	18.681647563750154	24.534173526730925	35.74781279381051	21.03636611570841
26	18.01863162352063	32.79538946265422	27.162481249955146	22.02349766387001
27	18.508824183789912	33.14555776449226	28.450402991394718	19.89521506032311
28	16.5509247629779	28.20021100528949	37.01441869478156	18.234445536951046
29	16.716284010248827	24.65855181471726	36.02829193371276	22.59687224132115
30	19.432295292572473	30.69122174933433	30.223349816626353	19.653133141466846
31	28.281814071325527	27.284060488182988	25.141567324323745	19.292558116167744
32	29.655788650212084	25.67561166414274	26.55738410857442	18.11121557707076
33	28.152627159395117	27.422075172428645	24.498575355443432	19.926722312732807
34	19.477510711748113	28.155354438646985	28.57348941026175	23.793645439343155
35	21.554405632549358	24.639604400967468	30.85227476620758	22.9537152002756
36	30.96617456022622	25.41551534812284	24.218813920607467	19.39949617104347
37	21.023303883502116	33.272232708690694	27.132840030717777	18.57162337708942
38	21.00988279876268	32.469623133069696	24.29388587054036	22.226608197627268
39	21.90428685236089	30.87689204998098	26.566929585955947	20.651891511702182
40	24.047138868753272	26.320541436702	25.380060717848608	24.25225897669612
41	18.445522596944013	25.231495769128635	27.32683571013328	28.996145923794074
42	21.900195933483094	25.397142098426073	25.117452434096734	27.5852095339941
43	21.411797635879513	26.01436845542693	26.70580551628114	25.868028392412423
44	19.44593168883179	28.98272483905464	28.51104906949538	23.06029440261819
45	18.464613551707064	36.00044497714109	23.542807518678273	21.99213395247357
46	22.101081581534885	32.64854700609332	25.679917894540417	19.570453517831382
47	21.991846870447056	28.21872779599951	26.735949129064903	23.053476204488526
48	21.88706193077017	24.844078574350657	31.067227433558454	22.20163206132072
49	21.297538989327723	25.35651999167462	31.93773190844954	21.408209110548114
50	20.507345711353373	32.92701657180998	26.772049693898794	19.793588022937854
51	19.839664688193036	32.729647678582964	25.023648381933928	22.407039251290076
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1432.0
1	2248.0
2	3064.0
3	6559.0
4	10054.0
5	7631.0
6	5208.0
7	5305.0
8	5402.0
9	5721.5
10	6041.0
11	6219.0
12	6397.0
13	6431.0
14	6465.0
15	6317.5
16	6170.0
17	6040.0
18	5910.0
19	5764.5
20	5619.0
21	5366.0
22	5113.0
23	5765.5
24	6418.0
25	6573.0
26	8144.5
27	9561.0
28	13027.5
29	16494.0
30	19255.5
31	22017.0
32	24682.0
33	27347.0
34	31270.0
35	35193.0
36	38062.0
37	40931.0
38	49064.0
39	57197.0
40	72173.5
41	87150.0
42	106603.0
43	126056.0
44	134757.0
45	143458.0
46	134781.0
47	126104.0
48	119030.0
49	111956.0
50	104421.0
51	96886.0
52	87798.5
53	78711.0
54	71619.5
55	64528.0
56	60255.5
57	55983.0
58	51489.0
59	46995.0
60	45927.0
61	44859.0
62	38942.0
63	33025.0
64	29066.0
65	25107.0
66	22635.5
67	20164.0
68	16920.0
69	13676.0
70	12453.0
71	11230.0
72	9320.0
73	7410.0
74	6172.5
75	3861.5
76	2788.0
77	2431.5
78	2075.0
79	1425.0
80	775.0
81	540.5
82	306.0
83	290.0
84	274.0
85	178.5
86	83.0
87	51.0
88	19.0
89	14.5
90	10.0
91	7.0
92	4.0
93	3.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1393330.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.66120416188149
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.46275030687677	13.406063102893661
2	6.922515658418788	2.3067489339747373
3	2.366026636374464	1.18262558523254
4	1.2097447130355812	0.806232145905702
5	0.6868118883763498	0.5721556546522861
6	0.47109046785109915	0.4709360678150058
7	0.34342133759061816	0.4005269113400599
8	0.2747883544823438	0.3662643900270233
9	0.236966994163401	0.35533399224454226
>10	3.728309791299711	15.70932724025046
>50	1.7364998779713086	20.775310789809733
>100	1.5498120636504331	41.724402289540066
>500	0.008229857241296318	0.8010655695243061
>1k	0.002598902286725153	0.741526912329932
>5k	4.331503811208589E-4	0.3814804144599533
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5286	0.37937889803564123	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3568	0.25607716764872646	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1644	0.11799071289644233	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1580	0.11339740047224993	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.021602922495029892	0.0
2	0.0	0.0	0.0	0.0809571314763911	0.0
3	0.0	0.0	0.0	0.1151916631379501	0.0
4	0.0	0.0	0.0	0.18229708683513596	0.0
5	0.0	0.0	0.0	0.31227347433845537	0.0
6	0.0	0.0	0.0	0.433852712566298	0.0
7	0.0	0.0	0.0	0.5048337436213962	0.0
8	0.0	0.0	0.0	0.6767958775020992	0.0
9	0.0	0.0	0.0	0.730982610006244	0.0
10	0.0	0.0	0.0	0.8354804676566212	0.0
11	7.177050662800628E-5	0.0	0.0	0.9605046902026081	0.0
12	7.177050662800628E-5	0.0	0.0	1.0690216962241537	0.0
13	1.4354101325601257E-4	0.0	0.0	1.1092849504424651	0.0
14	2.1531151988401885E-4	0.0	0.0	1.1254333144337665	0.0
15	2.1531151988401885E-4	0.0	0.0	1.1558640092440413	0.0
16	2.1531151988401885E-4	0.0	0.0	1.2249072366201832	0.0
17	2.1531151988401885E-4	0.0	0.0	1.301916990232034	0.0
18	2.1531151988401885E-4	0.0	0.0	1.414453144624748	0.0
19	2.1531151988401885E-4	0.0	0.0	1.4696446642216847	0.0
20	2.1531151988401885E-4	0.0	0.0	1.5212476584872212	0.0
21	2.8708202651202513E-4	0.0	0.0	1.6009846913509362	0.0
22	2.8708202651202513E-4	0.0	0.0	1.6767743463501108	0.0
23	2.8708202651202513E-4	0.0	0.0	1.7745975468840836	0.0
24	2.8708202651202513E-4	0.0	0.0	1.850746054416398	0.0
25	2.8708202651202513E-4	0.0	0.0	1.9118227555568315	0.0
26	2.8708202651202513E-4	0.0	0.0	1.9767032935485491	0.0
27	2.8708202651202513E-4	0.0	0.0	2.0370622896227024	0.0
28	2.8708202651202513E-4	0.0	0.0	2.10337823774698	0.0
29	2.8708202651202513E-4	0.0	0.0	2.1734980227225424	0.0
30	2.8708202651202513E-4	0.0	0.0	2.2662972877925545	0.0
31	2.8708202651202513E-4	0.0	0.0	2.3549338634781423	0.0
32	2.8708202651202513E-4	0.0	0.0	2.4368957820473254	0.0
33	2.8708202651202513E-4	0.0	0.0	2.5271113088787294	0.0
34	2.8708202651202513E-4	0.0	0.0	2.6094320799810524	0.0
35	2.8708202651202513E-4	0.0	0.0	2.7238342675460947	0.0
36	2.8708202651202513E-4	0.0	0.0	2.8162746800829668	0.0
37	2.8708202651202513E-4	0.0	0.0	2.911083519338563	0.0
38	2.8708202651202513E-4	0.0	0.0	3.002662685795899	0.0
39	2.8708202651202513E-4	0.0	0.0	3.0963949674520754	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGATAT	145	0.0	45.000004	9
ATGCGAG	60	0.0	45.000004	1
TATCCGG	30	2.1658343E-6	45.000004	2
TAGACGT	35	1.2121745E-7	45.0	22
CGTTACA	20	7.0338027E-4	45.0	35
ACCTAGT	20	7.0338027E-4	45.0	9
TACGATA	35	1.2121745E-7	45.0	42
CTACGAT	35	1.2121745E-7	45.0	41
TGACACG	50	2.1827873E-11	45.0	1
TCTAACG	50	2.1827873E-11	45.0	1
ACTACGT	20	7.0338027E-4	45.0	17
TCGTCGG	25	3.8912334E-5	45.0	33
GAACGTA	25	3.8912334E-5	45.0	20
TCGTAAG	20	7.0338027E-4	45.0	1
TTACGTA	25	3.8912334E-5	45.0	21
AACCTAG	25	3.8912334E-5	45.0	31
ACCGTTG	20	7.0338027E-4	45.0	1
ACGTATG	45	3.8562575E-10	45.0	1
TCGAGTA	25	3.8912334E-5	45.0	26
CACAACG	20	7.0338027E-4	45.0	12
>>END_MODULE