FastQCFastQC Report
Thu 26 May 2016
SRR1545548_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545548_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2254604
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91340.4051265765518024No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC75420.33451550693602955No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT55870.2478040489593738No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGTA207.03473E-445.00000415
AACCGGC207.03473E-445.00000434
TCCGAAC207.03473E-445.00000436
CACGCGA207.03473E-445.00000433
TAGCCGT351.2126293E-745.00000444
CCGTAGT351.2126293E-745.00000440
CGAGGTA207.03473E-445.00000421
CGCTACG207.03473E-445.00000433
CGATCAT351.2126293E-745.00000410
GTGCGTA207.03473E-445.00000421
CAATACG207.03473E-445.0000041
CCGTTAT351.2126293E-745.00000437
GACTATC207.03473E-445.00000428
TACCGTT351.2126293E-745.00000436
CCTTACG253.8920036E-545.029
TCGTTAG453.8562575E-1045.01
TACCCGC253.8920036E-545.014
ATCTACG1050.045.01
ACGTTGT453.8562575E-1045.014
ACGCATC253.8920036E-545.024