##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545548_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2254604
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.323620910811833	31.0	31.0	33.0	30.0	34.0
2	31.668413167012922	31.0	31.0	34.0	30.0	34.0
3	31.769356392519484	31.0	31.0	34.0	30.0	34.0
4	35.462807659349494	37.0	35.0	37.0	33.0	37.0
5	35.32177579743494	37.0	35.0	37.0	33.0	37.0
6	35.40674592966215	37.0	35.0	37.0	33.0	37.0
7	35.86800209704232	37.0	35.0	37.0	35.0	37.0
8	35.898438484097426	37.0	35.0	37.0	35.0	37.0
9	37.6214093472734	39.0	37.0	39.0	35.0	39.0
10	36.9455301241371	39.0	37.0	39.0	32.0	39.0
11	36.511467202222654	39.0	35.0	39.0	32.0	39.0
12	35.51053666187055	37.0	35.0	39.0	31.0	39.0
13	35.03901971255262	37.0	34.0	39.0	30.0	39.0
14	35.96268080780483	38.0	35.0	40.0	30.0	41.0
15	36.29819649038146	38.0	35.0	40.0	31.0	41.0
16	36.54259373264662	38.0	35.0	40.0	32.0	41.0
17	36.49460082568824	38.0	35.0	40.0	32.0	41.0
18	36.471193610940105	37.0	35.0	40.0	31.0	41.0
19	36.40620969358699	37.0	35.0	40.0	31.0	41.0
20	36.244088097067156	37.0	35.0	40.0	31.0	41.0
21	36.02582493422348	36.0	34.0	40.0	31.0	41.0
22	35.95404159666177	36.0	34.0	40.0	30.0	41.0
23	35.9211063228842	36.0	34.0	40.0	30.0	41.0
24	35.86844829513298	36.0	34.0	40.0	30.0	41.0
25	35.779100454004336	36.0	34.0	40.0	30.0	41.0
26	35.68139371703412	36.0	34.0	40.0	30.0	41.0
27	35.63579280441266	36.0	34.0	40.0	30.0	41.0
28	35.64654058983307	36.0	34.0	40.0	30.0	41.0
29	35.69161058882181	36.0	34.0	40.0	30.0	41.0
30	35.619120253490195	36.0	34.0	40.0	30.0	41.0
31	35.4232344127838	36.0	34.0	40.0	30.0	41.0
32	35.23499559124352	35.0	34.0	40.0	29.0	41.0
33	35.03255782390167	35.0	34.0	40.0	28.0	41.0
34	34.88512794264536	35.0	34.0	40.0	27.0	41.0
35	34.72347605167027	35.0	34.0	40.0	27.0	41.0
36	34.533338005255025	35.0	33.0	40.0	25.0	41.0
37	34.45774867781659	35.0	33.0	40.0	25.0	41.0
38	34.486863768537624	35.0	33.0	40.0	26.0	41.0
39	34.45944609341596	35.0	33.0	40.0	26.0	41.0
40	34.29674789896585	35.0	33.0	40.0	24.0	41.0
41	34.30690444973929	35.0	33.0	40.0	24.0	41.0
42	34.23861973100376	35.0	33.0	40.0	24.0	41.0
43	34.155490720321616	35.0	33.0	40.0	24.0	41.0
44	34.0828872830883	35.0	33.0	40.0	24.0	41.0
45	34.064309297774685	35.0	33.0	40.0	24.0	41.0
46	34.04367862382929	35.0	33.0	39.0	24.0	41.0
47	34.009544469893605	35.0	33.0	39.0	24.0	41.0
48	34.00119267064194	35.0	33.0	39.0	24.0	41.0
49	33.95488609086119	35.0	33.0	39.0	24.0	41.0
50	33.80618636354765	35.0	33.0	39.0	24.0	41.0
51	33.64558476787941	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	1.0
12	4.0
13	4.0
14	21.0
15	55.0
16	175.0
17	421.0
18	1158.0
19	2206.0
20	3812.0
21	6261.0
22	9601.0
23	13874.0
24	19766.0
25	28232.0
26	37728.0
27	43994.0
28	48927.0
29	55769.0
30	66827.0
31	82043.0
32	102232.0
33	133870.0
34	234845.0
35	377790.0
36	148815.0
37	175353.0
38	245758.0
39	414863.0
40	196.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.51706286336758	23.531094595769368	29.238172202302486	14.713670338560563
2	29.616376090878934	25.60316578875936	31.195766529288516	13.5846915910732
3	27.96584233861024	25.344184610689947	32.8758398370623	13.814133213637517
4	25.113234962769514	27.814463205068385	31.597256103510862	15.47504572865124
5	22.00909782826607	32.702195152674264	30.885822964919786	14.402884054139884
6	20.71312744943236	40.88026101257693	27.396474059302655	11.010137478688053
7	87.99061830813748	4.140904566833022	6.2108467828496705	1.657630342179824
8	89.27532285048727	2.9031705789575466	5.977679450582009	1.8438271199731748
9	85.86204051798009	4.31477101965578	7.45354838366294	2.369640078701182
10	54.74655416206128	21.97596562411847	13.242369835234925	10.03511037858533
11	46.43658043718542	19.301482655047185	20.53504739634987	13.726889511417525
12	44.04400950233389	19.90513633436293	24.170186870953835	11.880667292349344
13	20.86676862100839	42.652634342882386	22.940525254102273	13.540071782006951
14	14.6245637814889	43.646866589432115	27.181935275551716	14.54663435352727
15	12.270225724783598	23.54262655437496	48.67014340434063	15.517004316500813
16	14.649224431430085	17.464973893419863	47.66007689155169	20.225724783598363
17	14.68798955381965	18.81532189244763	28.655808292720142	37.84088026101258
18	18.90438409583235	22.324363835068155	37.369711044600294	21.401541024499203
19	27.511971060106344	24.16876755297161	25.283508766949765	23.03575261997229
20	30.49582099561608	21.292652723050256	27.74779961359068	20.463726667742982
21	20.302944552568878	28.90316880481007	29.41824817129749	21.375638471323565
22	23.107783007570287	24.19852887691142	24.331013339814884	28.362674775703407
23	17.633340489061496	30.473688505830737	24.796017393741874	27.096953611365898
24	18.261832233066205	22.853503320317003	42.58832149681274	16.296342949804046
25	15.820560949949524	25.636697176089456	37.41863316130017	21.12410871266085
26	16.52197015529113	33.341065659424004	27.958967517133832	22.17799666815104
27	15.946924604054635	36.526946638966315	27.057301415237443	20.468827341741612
28	13.169452373898032	29.374737204404855	37.427991789245475	20.02781863245164
29	14.268137553202248	22.87980505667514	37.352191338257185	25.49986605186543
30	16.332757326785547	33.658371935825535	31.798355720117588	18.210515017271327
31	27.4991971982663	29.42077633145333	24.604897356697673	18.475129113582696
32	29.771259165689408	25.381885244592844	26.334602440162442	18.51225314955531
33	28.356199137409497	29.25156701575975	23.60645150988821	18.785782336942543
34	19.232823147656973	26.699101039473007	27.304839342075148	26.763236470794872
35	18.963374499468642	26.015433308909238	31.90941735222682	23.1117748393953
36	31.13229640327082	25.538276344759435	25.393505910572323	17.935921341397425
37	19.562060565846597	33.22707668397643	28.89101589458725	18.319846855589716
38	19.80161482903428	33.35636768141988	25.049321299882376	21.792696189663463
39	19.453881923388764	33.49155771922697	27.24531669419552	19.809243663188745
40	24.49942428914346	27.083558797908637	25.04896647038682	23.36805044256109
41	16.576702604980742	24.66180313704757	28.49866317987549	30.262831078096198
42	20.324456090736998	25.117670331463977	24.96708956428712	29.590784013511907
43	20.550792955215194	25.260799679234136	27.579654786383774	26.608752579166893
44	17.915163815907363	29.185568729586215	29.432796180615313	23.466471273891113
45	15.763655169599627	38.79874248426775	23.019740938985294	22.41786140714733
46	20.395776819343887	34.075296593104596	26.340767602647734	19.18815898490378
47	19.71255262564956	29.892167316300334	26.562846513179256	23.832433544870852
48	21.102818942927453	25.618334749694405	31.280526425039607	21.998319882338542
49	20.720046624595717	24.36862526634389	33.156155138552045	21.755172970508347
50	19.730515868862113	33.72312831876463	27.200563824068443	19.34579198830482
51	17.238503967880835	33.622401095713485	24.355274806573572	24.78382012983211
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1828.0
1	2654.5
2	3481.0
3	9451.0
4	15421.0
5	11363.0
6	7305.0
7	7814.0
8	8323.0
9	9136.5
10	9950.0
11	10446.5
12	10943.0
13	10836.0
14	10729.0
15	10476.5
16	10224.0
17	9363.5
18	8503.0
19	8220.5
20	7938.0
21	7999.0
22	8060.0
23	8239.0
24	8418.0
25	10357.0
26	14354.0
27	16412.0
28	17871.5
29	19331.0
30	27972.0
31	36613.0
32	41878.5
33	47144.0
34	59737.5
35	72331.0
36	72938.0
37	73545.0
38	89523.0
39	105501.0
40	134021.0
41	162541.0
42	196931.0
43	231321.0
44	238033.0
45	244745.0
46	237277.5
47	229810.0
48	204737.5
49	179665.0
50	168600.5
51	157536.0
52	142239.5
53	126943.0
54	115876.5
55	104810.0
56	90318.5
57	75827.0
58	68492.0
59	61157.0
60	58032.0
61	54907.0
62	48569.5
63	42232.0
64	36032.0
65	29832.0
66	25637.5
67	21443.0
68	17994.0
69	14545.0
70	11610.0
71	8675.0
72	7460.5
73	6246.0
74	5252.5
75	3053.5
76	1848.0
77	1376.5
78	905.0
79	746.0
80	587.0
81	459.0
82	331.0
83	212.5
84	94.0
85	56.5
86	19.0
87	14.5
88	10.0
89	13.0
90	16.0
91	9.0
92	2.0
93	1.0
94	0.0
95	0.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2254604.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.87967458420135
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.78949207028923	11.872416772446178
2	8.566041294441645	2.549198138722452
3	3.074163650239872	1.372276642024496
4	1.5415536135048353	0.917512644922066
5	0.8700170804536247	0.647278551992343
6	0.5696772152223731	0.5085966948325769
7	0.3921322536005009	0.40843602292814796
8	0.2553414855790268	0.3039518570609968
9	0.21181033212662295	0.28365019340541825
>10	2.085442395947205	7.199556580893095
>50	0.8258267767026338	9.058431694223279
>100	1.7761132125674859	59.99161910996421
>500	0.04088547679571566	3.786520331856987
>1k	6.012570117017009E-4	0.10467415150441062
>5k	9.018855175525512E-4	0.9958806132233733
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9134	0.4051265765518024	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	7542	0.33451550693602955	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	5587	0.2478040489593738	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04009573299790118	0.0
2	0.0	0.0	0.0	0.14250839615293862	0.0
3	0.0	0.0	0.0	0.20136573872839753	0.0
4	0.0	0.0	0.0	0.32134246191348903	0.0
5	0.0	0.0	0.0	0.5387642353158248	0.0
6	0.0	0.0	0.0	0.7367590938364342	0.0
7	0.0	0.0	0.0	0.8556713285348558	0.0
8	0.0	0.0	0.0	1.1551030690977218	0.0
9	0.0	0.0	0.0	1.2472700305685611	0.0
10	0.0	0.0	0.0	1.4262371573899453	0.0
11	0.0	0.0	0.0	1.6582956474839927	0.0
12	0.0	0.0	0.0	1.842008618808447	0.0
13	4.435368694458096E-5	0.0	0.0	1.9089383324078197	0.0
14	4.435368694458096E-5	0.0	0.0	1.932933677044838	0.0
15	4.435368694458096E-5	0.0	0.0	1.9747148501466334	0.0
16	4.435368694458096E-5	0.0	0.0	2.0787685997186203	0.0
17	4.435368694458096E-5	0.0	0.0	2.2070394623623484	0.0
18	4.435368694458096E-5	0.0	0.0	2.37939788982899	0.0
19	8.870737388916192E-5	0.0	0.0	2.465577103562311	0.0
20	8.870737388916192E-5	0.0	0.0	2.544925849506166	0.0
21	8.870737388916192E-5	0.0	0.0	2.662418766222361	0.0
22	8.870737388916192E-5	0.0	0.0	2.7879397002755253	0.0
23	8.870737388916192E-5	0.0	0.0	2.9411373349821077	0.0
24	8.870737388916192E-5	0.0	0.0	3.0543279440646782	0.0
25	8.870737388916192E-5	0.0	0.0	3.1414829389107797	0.0
26	8.870737388916192E-5	0.0	0.0	3.2316096307821685	0.0
27	8.870737388916192E-5	0.0	0.0	3.3181436740110457	0.0
28	8.870737388916192E-5	0.0	0.0	3.4116412460902223	0.0
29	8.870737388916192E-5	0.0	0.0	3.5158724104099877	0.0
30	8.870737388916192E-5	0.0	0.0	3.651772107208184	0.0
31	8.870737388916192E-5	0.0	0.0	3.7768051507049574	0.0
32	8.870737388916192E-5	0.0	0.0	3.883963658363065	0.0
33	8.870737388916192E-5	0.0	0.0	3.989702848038946	0.0
34	8.870737388916192E-5	0.0	0.0	4.105288556216524	0.0
35	8.870737388916192E-5	0.0	0.0	4.278400996361223	0.0
36	8.870737388916192E-5	0.0	0.0	4.406716212691896	0.0
37	8.870737388916192E-5	0.0	0.0	4.535652380639793	0.0
38	8.870737388916192E-5	0.0	0.0	4.657358897615723	0.0
39	8.870737388916192E-5	0.0	0.0	4.78097262313027	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGTA	20	7.03473E-4	45.000004	15
AACCGGC	20	7.03473E-4	45.000004	34
TCCGAAC	20	7.03473E-4	45.000004	36
CACGCGA	20	7.03473E-4	45.000004	33
TAGCCGT	35	1.2126293E-7	45.000004	44
CCGTAGT	35	1.2126293E-7	45.000004	40
CGAGGTA	20	7.03473E-4	45.000004	21
CGCTACG	20	7.03473E-4	45.000004	33
CGATCAT	35	1.2126293E-7	45.000004	10
GTGCGTA	20	7.03473E-4	45.000004	21
CAATACG	20	7.03473E-4	45.000004	1
CCGTTAT	35	1.2126293E-7	45.000004	37
GACTATC	20	7.03473E-4	45.000004	28
TACCGTT	35	1.2126293E-7	45.000004	36
CCTTACG	25	3.8920036E-5	45.0	29
TCGTTAG	45	3.8562575E-10	45.0	1
TACCCGC	25	3.8920036E-5	45.0	14
ATCTACG	105	0.0	45.0	1
ACGTTGT	45	3.8562575E-10	45.0	14
ACGCATC	25	3.8920036E-5	45.0	24
>>END_MODULE