FastQCFastQC Report
Thu 26 May 2016
SRR1545547_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545547_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2144451
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC63740.29723225198430736No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61540.2869732159886143No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT36360.1695538858197273No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACTA253.891939E-545.00000445
GTCGACA502.1827873E-1145.00000423
GCGCAAA253.891939E-545.0000041
TAGCACG650.045.0000041
TCGCTAG253.891939E-545.00000417
TATTACG502.1827873E-1145.0000041
TGTACGA253.891939E-545.00000422
ACTATCG207.034656E-445.027
GACCGTT406.8193913E-945.09
TATATCG207.034656E-445.026
TCGATAG351.2125929E-745.01
CCCGTTA207.034656E-445.022
CCGTACA207.034656E-445.025
AACGATC351.2125929E-745.023
ACGTAGA351.2125929E-745.043
CCGTTCG207.034656E-445.036
CCGTTAT207.034656E-445.023
CTTACGA207.034656E-445.027
TTTACGT302.1663855E-644.99999613
CGCTCGT302.1663855E-644.99999618