##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545547_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2144451
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.319898193057337	31.0	31.0	33.0	30.0	34.0
2	31.656267734725578	31.0	31.0	34.0	30.0	34.0
3	31.769998941454013	31.0	31.0	34.0	30.0	34.0
4	35.4761395807132	37.0	35.0	37.0	33.0	37.0
5	35.32637071213098	37.0	35.0	37.0	33.0	37.0
6	35.39529138226987	37.0	35.0	37.0	33.0	37.0
7	35.87030246902354	37.0	35.0	37.0	35.0	37.0
8	35.901638228152564	37.0	35.0	37.0	35.0	37.0
9	37.62884066831091	39.0	37.0	39.0	35.0	39.0
10	36.90063703950335	39.0	37.0	39.0	32.0	39.0
11	36.51551749142321	39.0	35.0	39.0	32.0	39.0
12	35.5991491528601	37.0	35.0	39.0	31.0	39.0
13	35.16255069479321	37.0	35.0	39.0	30.0	39.0
14	36.12093258367759	38.0	35.0	40.0	30.0	41.0
15	36.40294742104156	38.0	35.0	40.0	31.0	41.0
16	36.612999317774104	38.0	35.0	40.0	32.0	41.0
17	36.58143646089372	38.0	35.0	40.0	32.0	41.0
18	36.54394154960873	38.0	35.0	40.0	31.0	41.0
19	36.49272517767951	37.0	35.0	40.0	31.0	41.0
20	36.34722033751296	37.0	35.0	40.0	31.0	41.0
21	36.142418735611116	37.0	35.0	40.0	31.0	41.0
22	36.04859751983142	37.0	34.0	40.0	30.0	41.0
23	36.03120332430072	37.0	34.0	40.0	30.0	41.0
24	35.96229198055819	37.0	34.0	40.0	30.0	41.0
25	35.874915304663055	36.0	34.0	40.0	30.0	41.0
26	35.77166230424477	36.0	34.0	40.0	30.0	41.0
27	35.711681451336496	36.0	34.0	40.0	30.0	41.0
28	35.73299553125718	36.0	34.0	40.0	30.0	41.0
29	35.75642530419208	36.0	34.0	40.0	30.0	41.0
30	35.68256304294199	36.0	34.0	40.0	30.0	41.0
31	35.520751465060286	36.0	34.0	40.0	30.0	41.0
32	35.31694965284821	36.0	34.0	40.0	29.0	41.0
33	35.14155697658748	36.0	34.0	40.0	28.0	41.0
34	34.994937165736125	36.0	34.0	40.0	27.0	41.0
35	34.863589328923815	36.0	34.0	40.0	27.0	41.0
36	34.66464377129624	36.0	33.0	40.0	25.0	41.0
37	34.59574548450862	36.0	33.0	40.0	25.0	41.0
38	34.62872268939696	35.0	33.0	40.0	26.0	41.0
39	34.57821885414962	36.0	33.0	40.0	26.0	41.0
40	34.41069998801558	35.0	33.0	40.0	24.0	41.0
41	34.44363429148066	35.0	33.0	40.0	25.0	41.0
42	34.375543670617795	35.0	33.0	40.0	25.0	41.0
43	34.28959813024406	35.0	33.0	40.0	24.0	41.0
44	34.217877209598164	35.0	33.0	40.0	24.0	41.0
45	34.17416345722052	35.0	33.0	40.0	24.0	41.0
46	34.15570325458591	35.0	33.0	40.0	24.0	41.0
47	34.10518076654584	35.0	33.0	40.0	24.0	41.0
48	34.10335325917916	35.0	33.0	40.0	24.0	41.0
49	34.08030213793647	35.0	33.0	39.0	24.0	41.0
50	33.929331563183304	35.0	33.0	39.0	24.0	41.0
51	33.75503846905338	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	3.0
13	6.0
14	21.0
15	65.0
16	170.0
17	526.0
18	1152.0
19	2227.0
20	3981.0
21	6271.0
22	9298.0
23	13484.0
24	19057.0
25	26855.0
26	34613.0
27	40703.0
28	45847.0
29	52198.0
30	61818.0
31	76229.0
32	95780.0
33	123316.0
34	214982.0
35	335567.0
36	144819.0
37	175016.0
38	248221.0
39	412053.0
40	171.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.15170083158814	23.348400126652464	28.16497089464856	13.334928147110844
2	30.285653530903712	26.263505204828647	29.828706741259186	13.622134523008453
3	27.62427306569374	25.65220655543074	31.533758523743373	15.189761855132152
4	25.785154335538557	28.285467935616154	31.377075064900062	14.552302663945223
5	22.77422053476624	32.47288000518548	30.61632091383762	14.136578546210663
6	20.23221794296069	41.257599264333855	27.649594231810383	10.860588560895073
7	88.12525909894887	4.354401196390125	6.072370037832527	1.4479696668284796
8	89.39677334665143	3.325279990076714	5.686910076285259	1.5910365869865994
9	86.168674406643	4.416421732182269	6.982020106777911	2.432883754396813
10	52.14378878323637	20.83824717841536	15.140005530553042	11.877958507795235
11	45.623798352119024	21.044453801928793	19.144853391380824	14.186894454571355
12	41.00140315633232	20.82523685549355	24.29372366167378	13.87963632650035
13	21.007241480453505	41.28585824530381	23.792569753284173	13.914330520958512
14	15.702527126989613	42.88659428450452	27.047668610753988	14.36320997775188
15	14.782105070248749	23.69175140863559	47.1224103511808	14.403733169934869
16	15.681216311307649	18.142825366492403	46.4370134827049	19.73894483949505
17	16.024334433381785	19.563701851895893	29.047247990278162	35.36471572444416
18	20.32786013762963	23.58645639373434	35.21274209576251	20.872941372873523
19	27.885085739893334	24.319651043553804	25.904345681015794	21.89091753553707
20	31.077138157971433	22.04461654754527	26.717793971510655	20.16045132297264
21	21.51445754647693	28.406617824328933	29.2465997124672	20.83232491672694
22	22.97716291955377	24.203444144911682	24.645841756235047	28.173551179299505
23	18.759486693797154	30.659175705110535	24.79245270700986	25.788884894082447
24	18.598419828664774	23.98590594982119	40.25114120117457	17.16453302033947
25	17.644049689174526	25.175347909558205	36.03990018890616	21.140702212361113
26	17.18174022162316	33.49523957413809	27.60310214595717	21.719918058281586
27	17.20594222017663	35.76542434403957	27.516273395848167	19.51236003993563
28	15.44385952395275	29.512914960519034	36.39155196364944	18.65167355187878
29	15.599936767032682	23.59816102116579	37.04197484577637	23.75992736602515
30	17.182113277477544	31.954332367584993	32.25184441146009	18.611709943477376
31	28.26681514289671	28.27348351629391	24.917006730393933	18.542694610415438
32	29.888349045979602	24.85027636444013	27.012647992423233	18.248726597157034
33	28.774870584592517	27.57750118794974	24.276889516244484	19.370738711213267
34	20.191041903032524	27.381040648632215	27.328206613254398	25.09971083508087
35	19.901364032099593	25.960024267283327	31.207008227280546	22.93160347333653
36	31.339349791624986	24.591468865457873	25.913112493593932	18.156068849323205
37	20.231098775397527	32.88212227745003	28.658337262077797	18.228441685074642
38	20.583869717703973	33.22696578285071	24.66039093455621	21.528773564889104
39	20.075021532317596	33.03973837592932	27.02136817301957	19.863871918733516
40	24.022045735715107	26.355323576990102	25.891801677911968	23.73082900938282
41	17.57661984349374	24.6808623745658	28.21691892237221	29.52559885956825
42	21.390929426692427	25.24095910794884	25.72103536056548	27.647076104793257
43	22.041771996655555	25.83957385829753	26.45301757885818	25.665636566188738
44	19.184956895727627	29.18145483389455	29.331330023395264	22.30225824698256
45	17.14616001951082	37.973588578148906	23.20985650872881	21.670394893611462
46	21.88140461125015	33.214235251819694	25.72103536056548	19.183324776364675
47	21.22165533276349	28.636466862614252	25.9810086590927	24.160869145529553
48	22.326600141481435	25.437839335102552	31.089822057020655	21.14573846639536
49	22.378128481368893	24.182972704902095	31.86419274676829	21.57470606696073
50	19.60287271660672	34.012108460393826	26.68393915272487	19.70107967027458
51	18.295638370846433	33.536881933884246	25.002483153030774	23.16499654223855
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1476.0
1	2134.0
2	2792.0
3	7312.0
4	11832.0
5	9243.0
6	6654.0
7	7112.0
8	7570.0
9	8135.5
10	8701.0
11	8950.0
12	9199.0
13	9059.0
14	8919.0
15	8891.0
16	8863.0
17	8383.0
18	7903.0
19	7631.0
20	7359.0
21	7963.0
22	8567.0
23	8756.5
24	8946.0
25	8874.0
26	12620.5
27	16439.0
28	20968.5
29	25498.0
30	29109.5
31	32721.0
32	39252.5
33	45784.0
34	53563.5
35	61343.0
36	65672.5
37	70002.0
38	82461.0
39	94920.0
40	123371.5
41	151823.0
42	184666.5
43	217510.0
44	223701.5
45	229893.0
46	213406.5
47	196920.0
48	192619.0
49	188318.0
50	170066.0
51	151814.0
52	139591.5
53	127369.0
54	114962.0
55	102555.0
56	91185.5
57	79816.0
58	70859.0
59	61902.0
60	56203.0
61	50504.0
62	46198.0
63	41892.0
64	34426.5
65	26961.0
66	24183.0
67	21405.0
68	17783.5
69	14162.0
70	12971.5
71	11781.0
72	9262.0
73	6743.0
74	5530.0
75	3325.5
76	2334.0
77	1536.5
78	739.0
79	868.0
80	997.0
81	597.5
82	198.0
83	147.5
84	97.0
85	78.5
86	60.0
87	46.5
88	33.0
89	21.0
90	9.0
91	7.5
92	6.0
93	4.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2144451.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.439256402135268
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.47519356701473	12.42477147492607
2	7.69036957074195	2.3746717525972785
3	2.725836543938795	1.2625466793654394
4	1.2904943381682112	0.7969709188993148
5	0.7547673486860355	0.5826523310161767
6	0.5077173115257647	0.4703266651469441
7	0.34969899979671054	0.37793647650021645
8	0.25327305746332457	0.31282781391432063
9	0.2210133142785902	0.3071053104689572
>10	2.6758623703001883	10.039623619778787
>50	1.205938367331829	13.604448769514239
>100	1.830664357278509	54.86518711134563
>500	0.017649357167903126	1.7257730269236902
>1k	9.128977845467135E-4	0.26657327620300597
>5k	6.085985230311423E-4	0.5885847733999399
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	6374	0.29723225198430736	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6154	0.2869732159886143	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	3636	0.1695538858197273	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.021077655772969398	0.0
2	0.0	0.0	0.0	0.08188576003835014	0.0
3	0.0	0.0	0.0	0.12184936843975451	0.0
4	0.0	0.0	0.0	0.2004242577704037	0.0
5	0.0	0.0	0.0	0.33416478156880247	0.0
6	0.0	0.0	0.0	0.4763456940727487	0.0
7	0.0	0.0	0.0	0.557392078438724	0.0
8	0.0	0.0	0.0	0.7502153231759551	0.0
9	0.0	0.0	0.0	0.8129819706768772	0.0
10	4.6631981798604867E-5	0.0	0.0	0.9479349260020397	0.0
11	4.6631981798604867E-5	0.0	0.0	1.098602859193332	0.0
12	4.6631981798604867E-5	0.0	0.0	1.2268408091394953	0.0
13	4.6631981798604867E-5	0.0	0.0	1.2744986945376695	0.0
14	4.6631981798604867E-5	0.0	0.0	1.2916126318577574	0.0
15	4.6631981798604867E-5	0.0	0.0	1.3225762677720312	0.0
16	4.6631981798604867E-5	0.0	0.0	1.4024102206112428	0.0
17	4.6631981798604867E-5	0.0	0.0	1.4994513747341394	0.0
18	4.6631981798604867E-5	0.0	0.0	1.6425649268740576	0.0
19	4.6631981798604867E-5	0.0	0.0	1.7071502216651255	0.0
20	9.326396359720973E-5	0.0	0.0	1.773414267800943	0.0
21	9.326396359720973E-5	0.0	0.0	1.8767507394666514	0.0
22	9.326396359720973E-5	0.0	0.0	1.977335924206242	0.0
23	9.326396359720973E-5	0.0	0.0	2.104221546680246	0.0
24	9.326396359720973E-5	0.0	0.0	2.1960865508234977	0.0
25	9.326396359720973E-5	0.0	0.0	2.272050049173425	0.0
26	9.326396359720973E-5	0.0	0.0	2.34740733175997	0.0
27	9.326396359720973E-5	0.0	0.0	2.4218319747105435	0.0
28	9.326396359720973E-5	0.0	0.0	2.502785095112922	0.0
29	9.326396359720973E-5	0.0	0.0	2.5902666929671043	0.0
30	1.398959453958146E-4	0.0	0.0	2.705028000173471	0.0
31	1.398959453958146E-4	0.0	0.0	2.8114421826378875	0.0
32	1.398959453958146E-4	0.0	0.0	2.905079202089486	0.0
33	1.398959453958146E-4	0.0	0.0	3.004078899447924	0.0
34	1.398959453958146E-4	0.0	0.0	3.1031252287881608	0.0
35	1.398959453958146E-4	0.0	0.0	3.2456791971464956	0.0
36	1.398959453958146E-4	0.0	0.0	3.3491089327758012	0.0
37	1.398959453958146E-4	0.0	0.0	3.457761450366551	0.0
38	1.398959453958146E-4	0.0	0.0	3.5700046305557924	0.0
39	1.398959453958146E-4	0.0	0.0	3.6799628436369027	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACTA	25	3.891939E-5	45.000004	45
GTCGACA	50	2.1827873E-11	45.000004	23
GCGCAAA	25	3.891939E-5	45.000004	1
TAGCACG	65	0.0	45.000004	1
TCGCTAG	25	3.891939E-5	45.000004	17
TATTACG	50	2.1827873E-11	45.000004	1
TGTACGA	25	3.891939E-5	45.000004	22
ACTATCG	20	7.034656E-4	45.0	27
GACCGTT	40	6.8193913E-9	45.0	9
TATATCG	20	7.034656E-4	45.0	26
TCGATAG	35	1.2125929E-7	45.0	1
CCCGTTA	20	7.034656E-4	45.0	22
CCGTACA	20	7.034656E-4	45.0	25
AACGATC	35	1.2125929E-7	45.0	23
ACGTAGA	35	1.2125929E-7	45.0	43
CCGTTCG	20	7.034656E-4	45.0	36
CCGTTAT	20	7.034656E-4	45.0	23
CTTACGA	20	7.034656E-4	45.0	27
TTTACGT	30	2.1663855E-6	44.999996	13
CGCTCGT	30	2.1663855E-6	44.999996	18
>>END_MODULE