FastQCFastQC Report
Thu 26 May 2016
SRR1545546_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545546_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1746365
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69900.40025996856327284No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC64370.3685941942262929No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT59570.3411085311489866No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAATA207.0342934E-445.00000419
TACGAAC302.1661508E-645.00000426
CGAGAAT207.0342934E-445.00000435
TCGACGA302.1661508E-645.00000428
ACGGTAC302.1661508E-645.0000045
CTTAACG502.1827873E-1145.01
ACCGGGT253.891641E-545.04
TCACCGT253.891641E-545.039
GGCCGTA453.8562575E-1045.08
TAACGCG453.8562575E-1045.01
TCGTGAC351.212411E-745.012
CTACGTC253.891641E-545.045
TATAGCG1450.045.01
ACGTAAG750.045.01
CCAACGT253.891641E-545.022
GCTAACG253.891641E-545.01
CGTAAGG2300.044.021742
CGACGGT5800.043.83620528
CGGTCTA5550.043.78378331
ACGGGTA1500.043.55
Produced by FastQC (version 0.11.5)