##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545546_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1746365
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.269834771081648	31.0	31.0	33.0	30.0	34.0
2	31.607501868166164	31.0	31.0	34.0	30.0	34.0
3	31.714562534178135	31.0	31.0	34.0	30.0	34.0
4	35.41963449794287	37.0	35.0	37.0	33.0	37.0
5	35.26226590661173	37.0	35.0	37.0	33.0	37.0
6	35.350640330057004	37.0	35.0	37.0	33.0	37.0
7	35.84025790713854	37.0	35.0	37.0	35.0	37.0
8	35.880315397983814	37.0	35.0	37.0	35.0	37.0
9	37.582999544768704	39.0	37.0	39.0	35.0	39.0
10	36.88180993091364	39.0	37.0	39.0	32.0	39.0
11	36.43802870533938	39.0	35.0	39.0	32.0	39.0
12	35.45547866568558	37.0	35.0	39.0	31.0	39.0
13	34.96798263822282	37.0	34.0	39.0	29.0	39.0
14	35.92805284118727	38.0	35.0	40.0	30.0	41.0
15	36.2669138467617	38.0	35.0	40.0	31.0	41.0
16	36.507150567034955	38.0	35.0	40.0	32.0	41.0
17	36.47319832910073	38.0	35.0	40.0	32.0	41.0
18	36.44786055606932	37.0	35.0	40.0	31.0	41.0
19	36.37737471834353	37.0	35.0	40.0	31.0	41.0
20	36.198352005451326	37.0	35.0	40.0	31.0	41.0
21	36.00514268208536	36.0	34.0	40.0	31.0	41.0
22	35.93195924105213	36.0	34.0	40.0	30.0	41.0
23	35.9339467980634	36.0	34.0	40.0	30.0	41.0
24	35.88036693360208	36.0	34.0	40.0	30.0	41.0
25	35.77126774757854	36.0	34.0	40.0	30.0	41.0
26	35.676933516189344	36.0	34.0	40.0	30.0	41.0
27	35.611444342963814	36.0	34.0	40.0	30.0	41.0
28	35.67120905423551	36.0	34.0	40.0	30.0	41.0
29	35.6805450177941	36.0	34.0	40.0	30.0	41.0
30	35.62544771568372	36.0	34.0	40.0	30.0	41.0
31	35.43000690004667	36.0	34.0	40.0	29.0	41.0
32	35.24861812965789	36.0	34.0	40.0	29.0	41.0
33	35.082955166875195	36.0	34.0	40.0	28.0	41.0
34	34.922953678068446	36.0	34.0	40.0	27.0	41.0
35	34.82551872031333	36.0	34.0	40.0	27.0	41.0
36	34.61516120627704	35.0	33.0	40.0	25.0	41.0
37	34.5590497977227	35.0	33.0	40.0	25.0	41.0
38	34.60351587440197	35.0	33.0	40.0	26.0	41.0
39	34.57926722077	35.0	33.0	40.0	26.0	41.0
40	34.39784466592035	35.0	33.0	40.0	24.0	41.0
41	34.391510365817	35.0	33.0	40.0	24.0	41.0
42	34.31953743919513	35.0	33.0	40.0	24.0	41.0
43	34.25002505203666	35.0	33.0	40.0	24.0	41.0
44	34.20708729274808	35.0	33.0	40.0	24.0	41.0
45	34.152271146066255	35.0	33.0	40.0	24.0	41.0
46	34.14445891895451	35.0	33.0	40.0	24.0	41.0
47	34.12148147723987	35.0	33.0	40.0	24.0	41.0
48	34.14189129992871	35.0	33.0	40.0	24.0	41.0
49	34.115981481534504	35.0	33.0	40.0	24.0	41.0
50	33.98055561122675	35.0	33.0	39.0	24.0	41.0
51	33.81055048629582	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	1.0
14	15.0
15	53.0
16	169.0
17	446.0
18	960.0
19	1873.0
20	3254.0
21	5034.0
22	7725.0
23	11307.0
24	15763.0
25	22586.0
26	29094.0
27	33898.0
28	37699.0
29	43072.0
30	51741.0
31	63190.0
32	79129.0
33	102556.0
34	176514.0
35	287249.0
36	112320.0
37	132586.0
38	189321.0
39	338597.0
40	211.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.558428507213556	23.86912243431356	29.41635912309282	14.156089935380061
2	28.595797556639074	26.85435175349941	31.093557188789283	13.456293501072228
3	27.55523616197072	25.555711434894768	32.97369106687319	13.91536133626132
4	25.31692973691067	28.293455262788708	31.58245842077687	14.807156579523753
5	21.98005571572953	33.08288931580741	30.852714066074387	14.084340902388675
6	19.90420101181597	42.217634915953994	27.356022366458326	10.522141705771702
7	88.33852029787587	4.139169074048095	5.943030237092475	1.5792803909835573
8	89.57657763411429	3.278524248939941	5.510932708797989	1.6339654081477812
9	85.58943863396254	4.775691221480045	7.275168707572586	2.359701436984823
10	51.93593550031065	26.256195010779532	13.068115771903354	8.739753717006469
11	45.04476441064726	19.755778431198518	22.368863324677257	12.830593833476964
12	44.55237020897693	20.289172080292495	23.718752952561463	11.43970475816911
13	19.832795549613053	45.81934475324459	21.580654674137424	12.767205023004927
14	13.482404880995668	45.65139589948264	25.50308784246134	15.363111377060351
15	11.531609944083854	23.613162196906146	49.38784274765012	15.467385111359883
16	12.9417962453439	18.015306078626175	48.67630764473635	20.366590031293573
17	13.071436956191862	19.14908968056506	29.391450240929018	38.38802312231407
18	18.681318052068153	23.209409258660134	38.80042259206981	19.3088500972019
19	27.362893782227655	26.94534075064491	24.39936668451326	21.29239878261417
20	30.628591388398185	21.828426474419725	27.756797691204298	19.78618444597779
21	18.40972534378552	29.604979485960836	29.32416762818769	22.661127542065948
22	21.132752889573485	25.500911894134386	23.51232417049128	29.85401104580085
23	15.681143403584016	33.13287886552926	24.785082156364794	26.400895574521936
24	16.257426139438206	22.81063809684688	45.63965723087671	15.29227853283821
25	14.157521480332003	29.226421738869025	36.8622252507351	19.753831530063877
26	14.952086190458466	35.95817598268403	27.67170665926081	21.418031167596695
27	13.926756434078785	37.671277195775225	27.778728959868072	20.62323741027792
28	12.028585089600398	29.64941464126915	39.205549813469695	19.116450455660758
29	12.547720550973022	22.312689500763014	40.323128326552585	24.816461621711383
30	15.556140898380349	35.703704552026636	31.437815118832546	17.302339430760465
31	27.150796082147778	30.853343945853247	24.347258448262536	17.648601523736446
32	28.138046742805773	25.61692429703985	26.475794006407593	19.769234953746782
33	27.26726657943786	31.19502509498301	22.41123705525477	19.12647127032436
34	16.01852991785795	27.376579351968232	28.286011229038603	28.318879501135214
35	17.86711254520103	25.993134310410483	33.10510689346156	23.034646250926922
36	29.073360952607274	26.984164249741603	24.750266983133535	19.192207814517584
37	15.820346834710957	35.93361067130869	30.01125194332227	18.23479055065808
38	16.896525067783656	34.1089634755621	27.905678366206377	21.088833090447874
39	17.298903722875803	35.2809979586169	27.623148654490898	19.7969496640164
40	24.489897587274136	27.359057241756446	25.450120679239447	22.700924491729964
41	14.855027442716729	24.684186868151848	29.81358421635798	30.647201472773446
42	19.03370715743845	25.73614336063767	24.835701585865497	30.394447896058384
43	19.203946483123516	26.19171822614402	27.3638099709969	27.240525319735564
44	17.544385051234993	30.01818062088968	29.487478276305357	22.949956051569977
45	14.315850352016904	42.31280402435917	21.052586372264674	22.318759251359253
46	19.05804342162148	37.75424954118984	25.159746101187324	18.027960936001353
47	17.41915349883902	30.47993976058842	28.333710306837347	23.767196433735215
48	18.995456276322535	25.037778471281776	34.11904155202377	21.847723700371915
49	18.887002430763328	25.392458048575183	33.73097834645106	21.989561174210433
50	17.04437503042033	35.926796517337436	28.27576136718269	18.753067085059538
51	15.401763090762813	35.132289069009055	25.331245186430102	24.134702653798033
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1409.0
1	1886.0
2	2363.0
3	6736.5
4	11110.0
5	8196.5
6	5283.0
7	5701.0
8	6119.0
9	6754.0
10	7389.0
11	7674.5
12	7960.0
13	7876.5
14	7793.0
15	7470.5
16	7148.0
17	7006.5
18	6865.0
19	6555.5
20	6246.0
21	6035.0
22	5824.0
23	5504.0
24	5184.0
25	7222.5
26	11023.0
27	12785.0
28	18477.5
29	24170.0
30	27869.5
31	31569.0
32	38737.0
33	45905.0
34	58932.5
35	71960.0
36	76308.0
37	80656.0
38	88260.0
39	95864.0
40	123337.5
41	150811.0
42	177827.5
43	204844.0
44	198277.5
45	191711.0
46	181405.5
47	171100.0
48	155795.5
49	140491.0
50	131147.5
51	121804.0
52	108034.0
53	94264.0
54	80175.0
55	66086.0
56	55679.0
57	45272.0
58	38145.0
59	31018.0
60	28201.0
61	25384.0
62	21628.5
63	17873.0
64	14418.5
65	10964.0
66	9367.0
67	7770.0
68	6179.0
69	4588.0
70	4168.5
71	3749.0
72	3066.5
73	2384.0
74	2029.0
75	1196.0
76	718.0
77	621.0
78	524.0
79	392.5
80	261.0
81	179.5
82	98.0
83	87.0
84	76.0
85	43.5
86	11.0
87	7.5
88	4.0
89	4.0
90	4.0
91	5.5
92	7.0
93	6.5
94	6.0
95	6.0
96	6.0
97	3.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1746365.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.173129962026334
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.39404486852813	14.455398206271344
2	8.832996078048657	3.3871236351699157
3	3.8892347279766613	2.2370640867696796
4	2.277556147532396	1.746715200498026
5	1.5321402417756922	1.4687961987807898
6	1.1236376919180424	1.292619089842556
7	0.8207236208726614	1.1015088452127455
8	0.6171809998651906	0.9466633216406926
9	0.50533137059648	0.8719905638101685
>10	3.096818448639064	11.632985105249565
>50	0.6401918800297257	8.965680743459783
>100	1.2553888146188437	49.038111491474716
>500	0.012948361484379549	1.52836164358014
>1k	9.03374057049736E-4	0.20784609840165097
>5k	9.03374057049736E-4	1.1191357698382227
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6990	0.40025996856327284	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	6437	0.3685941942262929	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	5957	0.3411085311489866	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04002599685632729	0.0
2	0.0	0.0	0.0	0.1374855771846092	0.0
3	0.0	0.0	0.0	0.19451832807001973	0.0
4	0.0	0.0	0.0	0.3079539500619859	0.0
5	0.0	0.0	0.0	0.5264649715265709	0.0
6	0.0	0.0	0.0	0.7310613760582696	0.0
7	0.0	0.0	0.0	0.8616755374735522	0.0
8	0.0	0.0	0.0	1.1793639932087507	0.0
9	0.0	0.0	0.0	1.2817480881717167	0.0
10	0.0	0.0	0.0	1.4577135936645547	0.0
11	0.0	0.0	0.0	1.688707687110083	0.0
12	5.726179807772144E-5	0.0	0.0	1.8820807792185483	0.0
13	5.726179807772144E-5	0.0	0.0	1.9631634852966018	0.0
14	5.726179807772144E-5	0.0	0.0	1.9862972517200013	0.0
15	5.726179807772144E-5	0.0	0.0	2.0303888362398466	0.0
16	5.726179807772144E-5	0.0	0.0	2.131513171645103	0.0
17	5.726179807772144E-5	0.0	0.0	2.2706020791758883	0.0
18	5.726179807772144E-5	0.0	0.0	2.462028270149711	0.0
19	5.726179807772144E-5	0.0	0.0	2.558228090920283	0.0
20	5.726179807772144E-5	0.0	0.0	2.650705894815803	0.0
21	5.726179807772144E-5	0.0	0.0	2.781892674211863	0.0
22	5.726179807772144E-5	0.0	0.0	2.9404505930890736	0.0
23	5.726179807772144E-5	0.0	0.0	3.127410363812834	0.0
24	5.726179807772144E-5	0.0	0.0	3.2615174949108576	0.0
25	5.726179807772144E-5	0.0	0.0	3.371231100027772	0.0
26	5.726179807772144E-5	0.0	0.0	3.4745313837599814	0.0
27	5.726179807772144E-5	3.4357078846632865E-4	0.0	3.582527134934564	0.0
28	1.7178539423316433E-4	3.4357078846632865E-4	0.0	3.6985967996381053	0.0
29	1.7178539423316433E-4	3.4357078846632865E-4	0.0	3.8229121632648386	0.0
30	1.7178539423316433E-4	3.4357078846632865E-4	0.0	3.9964726732384124	0.0
31	2.2904719231088577E-4	3.4357078846632865E-4	0.0	4.140428833605804	0.0
32	2.2904719231088577E-4	3.4357078846632865E-4	0.0	4.267320978146035	0.0
33	2.2904719231088577E-4	3.4357078846632865E-4	0.0	4.395930976628597	0.0
34	3.4357078846632865E-4	3.4357078846632865E-4	0.0	4.538684639236356	0.0
35	3.4357078846632865E-4	3.4357078846632865E-4	0.0	4.75673756631632	0.0
36	3.4357078846632865E-4	3.4357078846632865E-4	0.0	4.9076796660491935	0.0
37	3.4357078846632865E-4	3.4357078846632865E-4	0.0	5.057247482628202	0.0
38	3.4357078846632865E-4	3.4357078846632865E-4	0.0	5.206242681226433	0.0
39	3.4357078846632865E-4	3.4357078846632865E-4	0.0	5.361078583228592	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGAATA	20	7.0342934E-4	45.000004	19
TACGAAC	30	2.1661508E-6	45.000004	26
CGAGAAT	20	7.0342934E-4	45.000004	35
TCGACGA	30	2.1661508E-6	45.000004	28
ACGGTAC	30	2.1661508E-6	45.000004	5
CTTAACG	50	2.1827873E-11	45.0	1
ACCGGGT	25	3.891641E-5	45.0	4
TCACCGT	25	3.891641E-5	45.0	39
GGCCGTA	45	3.8562575E-10	45.0	8
TAACGCG	45	3.8562575E-10	45.0	1
TCGTGAC	35	1.212411E-7	45.0	12
CTACGTC	25	3.891641E-5	45.0	45
TATAGCG	145	0.0	45.0	1
ACGTAAG	75	0.0	45.0	1
CCAACGT	25	3.891641E-5	45.0	22
GCTAACG	25	3.891641E-5	45.0	1
CGTAAGG	230	0.0	44.02174	2
CGACGGT	580	0.0	43.836205	28
CGGTCTA	555	0.0	43.783783	31
ACGGGTA	150	0.0	43.5	5
>>END_MODULE