FastQCFastQC Report
Thu 26 May 2016
SRR1545545_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545545_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1570211
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67800.43178910350265026No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC38620.24595420615445948No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT19630.1250150457486287No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTAT207.034077E-445.043
CGACCGT207.034077E-445.016
CCTACGG207.034077E-445.026
CACGATT253.8914608E-545.026
CGACACT207.034077E-445.041
GCGATCG253.8914608E-545.09
CGTACGT207.034077E-445.020
TTACGTC207.034077E-445.033
CGTCGAT207.034077E-445.041
CCGGTAT253.8914608E-545.042
TATCGTG207.034077E-445.025
AATCGTC207.034077E-445.042
TAGTACG502.1827873E-1145.01
TACCGGT253.8914608E-545.040
CGTAAGG1700.043.6764682
CGGTCTA1850.042.56756631
ACTACGG800.042.18752
TGCGAAG1550.042.096771
GGCGATA4350.041.379318
TTACGGG4250.041.2941173