##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545545_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1570211
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.387701398092357	31.0	31.0	33.0	30.0	34.0
2	31.741968436089163	31.0	31.0	34.0	30.0	34.0
3	31.842922384316502	31.0	31.0	34.0	30.0	34.0
4	35.54946564506299	37.0	35.0	37.0	33.0	37.0
5	35.41487672675838	37.0	35.0	37.0	33.0	37.0
6	35.49371772328687	37.0	35.0	37.0	33.0	37.0
7	35.89166487815969	37.0	35.0	37.0	35.0	37.0
8	35.914796801194235	37.0	35.0	37.0	35.0	37.0
9	37.58812923868194	39.0	37.0	39.0	35.0	39.0
10	36.99250291839759	39.0	37.0	39.0	33.0	39.0
11	36.62857348471002	39.0	35.0	39.0	32.0	39.0
12	35.92705120522019	37.0	35.0	39.0	32.0	39.0
13	35.63110053362255	37.0	35.0	39.0	31.0	39.0
14	36.64405739101306	38.0	35.0	41.0	31.0	41.0
15	36.8429058260323	38.0	35.0	41.0	31.0	41.0
16	36.9875042271389	38.0	35.0	41.0	32.0	41.0
17	36.920671807801625	38.0	35.0	41.0	32.0	41.0
18	36.832036586165806	38.0	35.0	40.0	32.0	41.0
19	36.78564154753724	38.0	35.0	40.0	31.0	41.0
20	36.65156784661424	38.0	35.0	40.0	31.0	41.0
21	36.47535522296048	38.0	35.0	40.0	31.0	41.0
22	36.39366174354912	38.0	35.0	40.0	31.0	41.0
23	36.339358850498435	38.0	35.0	40.0	31.0	41.0
24	36.25380092229643	38.0	35.0	40.0	31.0	41.0
25	36.13177337313265	37.0	35.0	40.0	30.0	41.0
26	36.06179296922515	37.0	35.0	40.0	30.0	41.0
27	36.014181533564596	37.0	35.0	40.0	30.0	41.0
28	35.95960861310996	37.0	35.0	40.0	30.0	41.0
29	35.93141749739366	37.0	35.0	40.0	30.0	41.0
30	35.8519084377832	37.0	35.0	40.0	30.0	41.0
31	35.688563511528066	37.0	34.0	40.0	30.0	41.0
32	35.50183765111822	37.0	34.0	40.0	29.0	41.0
33	35.3497052306983	37.0	34.0	40.0	28.0	41.0
34	35.17227621001254	37.0	34.0	40.0	27.0	41.0
35	35.02922537162203	37.0	34.0	40.0	27.0	41.0
36	34.853634320483046	37.0	34.0	40.0	26.0	41.0
37	34.7717217622345	37.0	34.0	40.0	25.0	41.0
38	34.738958012649256	36.0	33.0	40.0	26.0	41.0
39	34.65836438542336	36.0	33.0	40.0	25.0	41.0
40	34.48216959376797	36.0	33.0	40.0	24.0	41.0
41	34.42353097768389	36.0	33.0	40.0	24.0	41.0
42	34.361238712504246	36.0	33.0	40.0	24.0	41.0
43	34.2919352876779	36.0	33.0	40.0	24.0	41.0
44	34.23320432731652	35.0	33.0	40.0	24.0	41.0
45	34.16184958581999	35.0	33.0	40.0	23.0	41.0
46	34.13578684648114	35.0	33.0	40.0	23.0	41.0
47	34.094787897932186	35.0	33.0	40.0	23.0	41.0
48	34.04271527839252	35.0	33.0	40.0	23.0	41.0
49	33.97747691233853	35.0	33.0	39.0	24.0	41.0
50	33.8547220723839	35.0	33.0	39.0	24.0	41.0
51	33.692192323197325	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	3.0
13	7.0
14	19.0
15	43.0
16	143.0
17	427.0
18	889.0
19	1672.0
20	2936.0
21	4481.0
22	6663.0
23	9464.0
24	13512.0
25	19223.0
26	25063.0
27	29971.0
28	32984.0
29	37244.0
30	44005.0
31	53378.0
32	65952.0
33	85786.0
34	143788.0
35	216975.0
36	114828.0
37	141323.0
38	198300.0
39	320952.0
40	179.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.375450815208914	23.522316427537447	28.06374429933302	15.038488457920623
2	32.0823125044978	25.295581294488446	29.3481576679822	13.273948533031549
3	29.108253604133456	25.406267055828803	31.50512892853253	13.980350411505205
4	26.522168039836686	27.64978719420511	30.265996098613495	15.562048667344708
5	23.542313740000548	32.12530035772262	29.754281430966923	14.578104471309908
6	22.159760694581813	41.37150994356809	26.184442727760793	10.284286634089304
7	86.354636415106	4.474494192181815	7.2332317121711664	1.937637680541023
8	87.34768766745361	3.3983967759746934	7.316341561739155	1.9375739948325417
9	82.77556328417009	5.3056563735701765	9.039358404698476	2.8794219375612577
10	48.61824302593728	23.76636006243747	15.23292092591378	12.382475985711475
11	40.78948625375825	22.417560442513775	21.844643809016752	14.948309494711221
12	38.1897719478465	20.99157374391085	26.07324748075259	14.745406827490063
13	22.129255240219308	35.28576732681149	25.98294114612622	16.602036286842978
14	16.95332665482537	37.48005841253182	28.707797869203567	16.858817063439243
15	16.12369293044056	23.716430466988196	43.96402776442147	16.195848838149775
16	17.493254091329128	18.991141954807347	42.75106976068822	20.76453419317531
17	17.810727348107992	19.464708883073676	30.103279113443985	32.621284655374346
18	21.627348171678836	22.82750534800737	33.92537690794422	21.619769572369574
19	27.514709806516446	24.53167122125625	26.22832218090435	21.725296791322947
20	29.34669289668713	22.268663256084693	27.170552237883953	21.214091609344223
21	22.80343215020147	26.178774699705965	29.02527112598243	21.992522024110137
22	23.16720491704618	23.437487063840464	27.039295992704165	26.356012026409186
23	21.182312440812094	28.111126466443043	26.415303421005206	24.291257671739658
24	20.554243983770334	23.2864882490315	37.228499864031015	18.930767903167155
25	19.71881485991373	24.63719844020963	33.79437540559835	21.849611294278283
26	19.46763842566381	31.042388570708013	27.03426482173415	22.45570818189403
27	19.85102639072074	31.919213405077407	27.965222508312575	20.264537695889278
28	17.65737216208522	27.93815608220806	35.05554349065189	19.348928265054823
29	18.484012658171416	24.841183764474966	34.99434152480144	21.680462052552173
30	20.63518851924996	28.522599828940187	30.78579885123719	20.056412800572662
31	27.34619742187515	26.75672250417301	25.089175913300828	20.80790416065102
32	27.628006681904534	25.617894665111884	27.522352091534195	19.231746561449384
33	26.784298415945372	26.762581589353278	25.941927549864317	20.511192444837032
34	20.223969899586745	27.72697427288435	28.348037301993173	23.70101852553574
35	21.93775231481629	24.796540082829633	30.52137578962318	22.7443318127309
36	28.629082333520785	25.955619977187776	25.783159078620642	19.632138610670797
37	21.64632651280624	31.64466431581488	27.88701645829764	18.821992713081233
38	21.76185238799117	30.678170003903933	25.15961230688105	22.40036530122385
39	22.342984477882272	29.62283412866169	26.5909486049964	21.44323278845964
40	24.201078708530254	26.04624474035655	26.802257785737076	22.950418765376117
41	19.01171243864678	25.089685398968676	28.90012870881684	26.998473453567705
42	22.465006295332284	26.670364683472474	25.033705661213684	25.830923359981554
43	22.16549240834512	26.587636948155374	27.063878676177914	24.18299196732159
44	20.83497058675554	28.82580748701926	28.3275941895707	22.0116277366545
45	19.27454335754876	33.64146601953495	24.573640103145372	22.51035051977091
46	21.97558162565413	31.054106741068555	27.004650967290385	19.965660665986928
47	22.538244860085683	28.21493417126743	27.270029314531612	21.976791654115274
48	23.068046268940925	25.01580997713046	30.117990512103148	21.79815324182546
49	21.13486658799359	25.757748480936637	30.685302803253826	22.422082127815944
50	20.47087939136842	31.562891866125	27.07413207524339	20.89209666726319
51	20.16397796219744	31.64937705824249	25.41760311193846	22.769041867621613
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1293.0
1	2748.0
2	4203.0
3	8812.5
4	13422.0
5	10121.5
6	6821.0
7	7079.0
8	7337.0
9	7621.0
10	7905.0
11	7916.0
12	7927.0
13	7707.0
14	7487.0
15	7525.0
16	7563.0
17	7334.5
18	7106.0
19	7410.5
20	7715.0
21	7123.0
22	6531.0
23	6575.5
24	6620.0
25	8571.0
26	12461.5
27	14401.0
28	18045.5
29	21690.0
30	22887.5
31	24085.0
32	27892.0
33	31699.0
34	38659.0
35	45619.0
36	46329.0
37	47039.0
38	53873.5
39	60708.0
40	78492.5
41	96277.0
42	114065.5
43	131854.0
44	135596.5
45	139339.0
46	133612.5
47	127886.0
48	122114.0
49	116342.0
50	110016.0
51	103690.0
52	97283.5
53	90877.0
54	80788.0
55	70699.0
56	67224.0
57	63749.0
58	62405.5
59	61062.0
60	59103.0
61	57144.0
62	52050.0
63	46956.0
64	42035.0
65	37114.0
66	30711.0
67	24308.0
68	20975.5
69	17643.0
70	15601.5
71	13560.0
72	11901.0
73	10242.0
74	8042.0
75	4625.0
76	3408.0
77	2840.0
78	2272.0
79	1794.5
80	1317.0
81	927.0
82	537.0
83	425.0
84	313.0
85	190.5
86	68.0
87	39.0
88	10.0
89	7.5
90	5.0
91	3.0
92	1.0
93	2.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1570211.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.232932382030775
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.23017087581354	13.023709388258562
2	6.44672727150461	2.0929857576745614
3	2.205411571240569	1.0740089073148922
4	1.1252883808166414	0.7306692078432575
5	0.71299911693293	0.5787033226809954
6	0.4837848065342052	0.4711947631154156
7	0.3990730543376969	0.4534688134588029
8	0.31790652423432864	0.4128444089361828
9	0.26409949938948385	0.3858398384145903
>10	4.195195877013585	17.410061531654005
>50	2.0733309540000664	24.28789275375263
>100	1.5381121598114462	37.53623060233397
>500	0.006319926696708561	0.66004447777023
>1k	0.0011849862556328552	0.44761710698600893
>5k	3.9499541854428504E-4	0.43472911980592177
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6780	0.43178910350265026	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3862	0.24595420615445948	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1963	0.1250150457486287	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.010953941858769299	0.0
2	0.0	0.0	0.0	0.03509082537315049	0.0
3	0.0	0.0	0.0	0.05018433828319888	0.0
4	0.0	0.0	0.0	0.08018030697785201	0.0
5	0.0	0.0	0.0	0.13202047368156253	0.0
6	0.0	0.0	0.0	0.1908660683182069	0.0
7	0.0	0.0	0.0	0.22831326490516243	0.0
8	0.0	0.0	0.0	0.3367700264486747	0.0
9	0.0	0.0	0.0	0.38447062210110616	0.0
10	0.0	0.0	0.0	0.47407641393417826	0.0
11	0.0	0.0	0.0	0.5938055458788659	0.0
12	0.0	0.0	0.0	0.6925183940247521	0.0
13	0.0	0.0	0.0	0.7306024476965197	0.0
14	0.0	0.0	0.0	0.7426390465994698	0.0
15	0.0	0.0	0.0	0.7636553303982713	0.0
16	0.0	0.0	0.0	0.81434915434932	0.0
17	0.0	0.0	0.0	0.8730036918605206	0.0
18	0.0	0.0	0.0	0.9536934845062224	6.368570848121686E-5
19	0.0	0.0	0.0	0.992923880930652	6.368570848121686E-5
20	0.0	0.0	0.0	1.0363575341148419	6.368570848121686E-5
21	0.0	0.0	0.0	1.0949483859175615	6.368570848121686E-5
22	0.0	0.0	0.0	1.1553861232662361	6.368570848121686E-5
23	0.0	0.0	0.0	1.2263320025143118	6.368570848121686E-5
24	0.0	0.0	0.0	1.2812290832251207	6.368570848121686E-5
25	6.368570848121686E-5	0.0	0.0	1.3269554219146344	6.368570848121686E-5
26	6.368570848121686E-5	0.0	0.0	1.367650589634132	6.368570848121686E-5
27	1.2737141696243372E-4	0.0	0.0	1.411402671360728	6.368570848121686E-5
28	1.2737141696243372E-4	0.0	0.0	1.45808429567746	6.368570848121686E-5
29	1.2737141696243372E-4	0.0	0.0	1.509223919587877	6.368570848121686E-5
30	1.2737141696243372E-4	0.0	0.0	1.5755207421168238	6.368570848121686E-5
31	1.2737141696243372E-4	0.0	0.0	1.6397796219743717	6.368570848121686E-5
32	1.9105712544365057E-4	0.0	0.0	1.702446359119889	6.368570848121686E-5
33	2.5474283392486744E-4	0.0	0.0	1.7635846392618573	6.368570848121686E-5
34	3.184285424060843E-4	0.0	0.0	1.8292446047059918	6.368570848121686E-5
35	3.184285424060843E-4	0.0	0.0	1.9156024254065218	6.368570848121686E-5
36	3.184285424060843E-4	0.0	0.0	1.9859114475697852	6.368570848121686E-5
37	3.184285424060843E-4	0.0	0.0	2.054500955604056	6.368570848121686E-5
38	3.184285424060843E-4	0.0	0.0	2.124427863516432	6.368570848121686E-5
39	3.184285424060843E-4	0.0	0.0	2.202060742155035	6.368570848121686E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACGTAT	20	7.034077E-4	45.0	43
CGACCGT	20	7.034077E-4	45.0	16
CCTACGG	20	7.034077E-4	45.0	26
CACGATT	25	3.8914608E-5	45.0	26
CGACACT	20	7.034077E-4	45.0	41
GCGATCG	25	3.8914608E-5	45.0	9
CGTACGT	20	7.034077E-4	45.0	20
TTACGTC	20	7.034077E-4	45.0	33
CGTCGAT	20	7.034077E-4	45.0	41
CCGGTAT	25	3.8914608E-5	45.0	42
TATCGTG	20	7.034077E-4	45.0	25
AATCGTC	20	7.034077E-4	45.0	42
TAGTACG	50	2.1827873E-11	45.0	1
TACCGGT	25	3.8914608E-5	45.0	40
CGTAAGG	170	0.0	43.676468	2
CGGTCTA	185	0.0	42.567566	31
ACTACGG	80	0.0	42.1875	2
TGCGAAG	155	0.0	42.09677	1
GGCGATA	435	0.0	41.37931	8
TTACGGG	425	0.0	41.294117	3
>>END_MODULE