FastQCFastQC Report
Thu 26 May 2016
SRR1545543_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545543_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1348369
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC57250.42458703811790394No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45670.3387055027221777No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGTA351.2121382E-745.00000441
CTCCGAT253.8911654E-545.00000420
CCGATCA351.2121382E-745.00000411
TGCGGAT253.8911654E-545.00000444
ACGGGTA650.045.0000045
TTATCCG502.1827873E-1145.0000041
ACCTCGT253.8911654E-545.00000428
TACGCGC351.2121382E-745.00000434
TGCGATA253.8911654E-545.0000041
TGTTACG453.8562575E-1045.0000041
ATGCGCA253.8911654E-545.00000412
TCGAGTA253.8911654E-545.00000412
CGGTACC351.2121382E-745.00000423
TATACGT253.8911654E-545.00000421
GCTAACG453.8562575E-1045.0000041
TACGTCT253.8911654E-545.00000431
ACAGTCG207.033722E-445.018
CACGATT207.033722E-445.018
TCGATTG207.033722E-445.01
GCGCATC207.033722E-445.019