##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545543_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1348369
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.319984366297355	31.0	31.0	33.0	30.0	34.0
2	31.656886208448874	31.0	31.0	34.0	30.0	34.0
3	31.755215375019745	31.0	31.0	34.0	30.0	34.0
4	35.459337911209765	37.0	35.0	37.0	33.0	37.0
5	35.31357143333909	37.0	35.0	37.0	33.0	37.0
6	35.394303784794815	37.0	35.0	37.0	33.0	37.0
7	35.85737435375628	37.0	35.0	37.0	35.0	37.0
8	35.88926992536909	37.0	35.0	37.0	35.0	37.0
9	37.60567841592324	39.0	37.0	39.0	35.0	39.0
10	36.967752892568726	39.0	37.0	39.0	32.0	39.0
11	36.531111290752015	39.0	35.0	39.0	32.0	39.0
12	35.43364835590258	37.0	35.0	39.0	31.0	39.0
13	34.955036047254126	37.0	34.0	39.0	29.0	39.0
14	35.8635633124167	38.0	35.0	40.0	30.0	41.0
15	36.21785060321025	38.0	35.0	40.0	31.0	41.0
16	36.44822448454392	37.0	35.0	40.0	32.0	41.0
17	36.413703518843874	37.0	35.0	40.0	31.0	41.0
18	36.370624065074175	37.0	35.0	40.0	31.0	41.0
19	36.318945333213684	37.0	35.0	40.0	31.0	41.0
20	36.15321770227586	36.0	35.0	40.0	31.0	41.0
21	35.94172515090454	36.0	34.0	40.0	31.0	41.0
22	35.84263432339367	36.0	34.0	40.0	30.0	41.0
23	35.844422409592624	36.0	34.0	40.0	30.0	41.0
24	35.78784294210264	36.0	34.0	40.0	30.0	41.0
25	35.66196419526109	35.0	34.0	40.0	30.0	41.0
26	35.53823693662491	35.0	34.0	40.0	30.0	41.0
27	35.47427818349428	35.0	34.0	40.0	30.0	41.0
28	35.51201191958581	36.0	34.0	40.0	30.0	41.0
29	35.56781711831108	36.0	34.0	40.0	30.0	41.0
30	35.497221457924354	36.0	34.0	40.0	30.0	41.0
31	35.28951051233008	36.0	34.0	40.0	29.0	41.0
32	35.09574604577827	35.0	34.0	40.0	29.0	41.0
33	34.91857792636882	35.0	34.0	40.0	28.0	41.0
34	34.780818158827444	35.0	34.0	40.0	27.0	41.0
35	34.60826450326283	35.0	33.0	40.0	27.0	41.0
36	34.43122839519449	35.0	33.0	40.0	25.0	41.0
37	34.34629096337872	35.0	33.0	40.0	25.0	41.0
38	34.376187082319454	35.0	33.0	40.0	26.0	41.0
39	34.34715200364292	35.0	33.0	40.0	25.0	41.0
40	34.15751919541312	35.0	33.0	40.0	24.0	41.0
41	34.178611344520675	35.0	33.0	40.0	24.0	41.0
42	34.10653389391183	35.0	33.0	40.0	24.0	41.0
43	34.01159919873566	35.0	33.0	39.0	24.0	41.0
44	33.95012418707342	35.0	33.0	39.0	24.0	41.0
45	33.927814270425976	35.0	33.0	39.0	24.0	41.0
46	33.92238400615855	35.0	33.0	39.0	24.0	41.0
47	33.87537313598874	35.0	33.0	39.0	24.0	41.0
48	33.847671520184754	35.0	33.0	39.0	24.0	41.0
49	33.824330728457866	35.0	33.0	39.0	24.0	41.0
50	33.66175505369821	35.0	33.0	39.0	24.0	41.0
51	33.49495946584355	35.0	33.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	4.0
13	3.0
14	8.0
15	30.0
16	111.0
17	316.0
18	684.0
19	1479.0
20	2505.0
21	3847.0
22	6069.0
23	8636.0
24	12265.0
25	16964.0
26	22583.0
27	26575.0
28	30130.0
29	34674.0
30	41134.0
31	49956.0
32	62840.0
33	81569.0
34	143591.0
35	228154.0
36	89400.0
37	105548.0
38	146988.0
39	232189.0
40	116.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.51456463327175	23.18868202991911	28.910483702903285	14.386269633905851
2	30.558548883873776	25.377919545762325	30.35608205172323	13.707449518640669
3	28.336234369078493	25.486865984014763	31.638668643375812	14.538231003530932
4	25.511414160367078	28.341129171614003	31.02689249011213	15.12056417790679
5	22.841744359296307	33.094501579315455	30.085236311425135	13.978517749963103
6	20.406209279507316	42.28115597436607	26.36674382160966	10.945890924516952
7	87.88647617974011	4.320701529032482	6.064289523120155	1.7285327681072467
8	89.41083635117687	3.044715504435359	6.012819932822543	1.5316282115652318
9	86.04291555204844	4.345843014783045	7.180230337541133	2.4310110956273836
10	56.69731356920843	20.939446101178536	12.013180368281978	10.350059961331059
11	49.40695017461837	18.35128217869144	19.224707776580445	13.01705987010974
12	42.9658350199389	19.60309084531015	24.57665520343467	12.85441893131628
13	20.5513475910526	42.46745512541448	23.304525689926127	13.676671593606796
14	14.387233761677997	44.39556234235584	27.76272667200151	13.454477223964656
15	13.576328141628888	21.4842524561155	50.79173431011837	14.147685092137241
16	15.3943764651961	16.19467667975161	49.22747408164976	19.18347277340253
17	15.38540266054767	16.704329452842657	28.42382166899417	39.48644621761551
18	20.783999038838775	22.134000410866758	36.26077134671592	20.821229203578547
19	29.664431620721032	22.726568172362313	25.40936494386922	22.19963526304743
20	32.24940650519257	22.065547339044432	24.70236263218748	20.98268352357552
21	21.711860774016607	27.80937562343839	28.687844351212465	21.790919251332536
22	22.73472617658816	23.779395699545155	25.08801374104566	28.397864382821027
23	18.780022382597046	30.675727490026837	24.34912104920834	26.195129078167774
24	19.89321914105115	22.15595285860176	40.430030651846785	17.5207973485003
25	17.115492865825306	23.600809570673903	37.529563494859346	21.75413406864145
26	17.23089154378364	34.29484065563655	26.865049552459304	21.609218248120506
27	17.41859980465288	35.115610044431456	27.64183988210942	19.82395026880624
28	14.497960128125165	27.963932721680784	38.41544859011146	19.122658560082588
29	14.922843820942191	24.41260515482038	37.749013808534606	22.915537215702823
30	17.866029254603156	31.159645467969078	31.276527419423022	19.697797858004744
31	30.607645236578414	25.779219189999175	24.110388180090165	19.50274739333224
32	32.2598635833366	24.452727702876587	26.22627782157555	17.061130892211256
33	30.24891554166552	25.492428259623296	24.309665974225155	19.948990224486028
34	20.473030750484476	27.021905724619895	27.243803439562907	25.261260085332726
35	20.312911376633547	24.706738288999524	32.19215214826209	22.78819818610484
36	33.41985762057716	22.615322660191683	25.494949824565825	18.469869894665333
37	21.123223687284415	32.80630153911874	28.44822151799693	17.62225325559991
38	21.416096038992293	33.517976162311655	22.557845812236856	22.508081986459196
39	20.86743317296675	31.104541857607227	26.634103869193076	21.393921100232948
40	25.11916248445344	25.30256925218542	24.415497538136815	25.162770725224327
41	17.242461077049384	23.32981550302625	27.70265409542937	31.725069324495003
42	21.744937772968676	24.096297081881886	25.20237412755707	28.956391017592363
43	22.34959421345344	24.268801789421147	26.715758075126317	26.6658459219991
44	19.42116735107378	29.054138740952958	30.106002140363653	21.41869176760961
45	17.192993905970845	37.73240114538379	22.29159821977515	22.783006728870212
46	22.60738714699018	32.80830395833782	25.165292290166864	19.419016604505146
47	21.547365743353637	27.456801513532277	26.346645465744167	24.64918727736992
48	23.351619623411693	23.611192485143164	31.516817725711583	21.520370165733564
49	21.744641118269552	23.236888418526384	32.8019258823067	22.216544580897367
50	20.11541351069329	33.82204722891137	26.383578975784815	19.67896028461052
51	18.427967418414397	33.5946614020346	24.369367732423395	23.608003447127604
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1022.0
1	1559.5
2	2097.0
3	5265.0
4	8433.0
5	6286.5
6	4140.0
7	4588.0
8	5036.0
9	5329.0
10	5622.0
11	5770.0
12	5918.0
13	5744.5
14	5571.0
15	5430.5
16	5290.0
17	5258.0
18	5226.0
19	5165.5
20	5105.0
21	4402.5
22	3700.0
23	4125.5
24	4551.0
25	4783.5
26	7117.0
27	9218.0
28	9616.5
29	10015.0
30	12893.5
31	15772.0
32	18226.5
33	20681.0
34	25261.0
35	29841.0
36	33748.5
37	37656.0
38	44328.5
39	51001.0
40	73555.0
41	96109.0
42	116633.5
43	137158.0
44	140317.5
45	143477.0
46	136542.5
47	129608.0
48	120219.0
49	110830.0
50	105383.5
51	99937.0
52	92741.5
53	85546.0
54	77705.5
55	69865.0
56	63307.0
57	56749.0
58	51252.5
59	45756.0
60	42660.5
61	39565.0
62	33385.0
63	27205.0
64	24859.0
65	22513.0
66	19521.0
67	16529.0
68	13703.0
69	10877.0
70	8507.5
71	6138.0
72	5258.5
73	4379.0
74	3759.5
75	2092.5
76	1045.0
77	800.0
78	555.0
79	403.0
80	251.0
81	196.5
82	142.0
83	93.5
84	45.0
85	32.5
86	20.0
87	13.0
88	6.0
89	4.5
90	3.0
91	3.5
92	4.0
93	4.0
94	4.0
95	3.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1348369.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.664918417844277
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.49540061579307	12.609538836580903
2	7.534107671426756	2.3604236404830967
3	2.494678307402359	1.1723679649267138
4	1.2109765646638553	0.7587939636552247
5	0.6707460038011928	0.5253590714320375
6	0.4544758222899097	0.42715960074324766
7	0.3342763363829385	0.3665488076918222
8	0.22973324853140614	0.28790020768886565
9	0.20879122176445555	0.29436277097720054
>10	2.9639335400320155	11.742303794877424
>50	1.5633697027916955	17.960148497992034
>100	1.8261569835223819	49.532899720112574
>500	0.011923197855330256	1.1132592036027167
>1k	9.538558284264203E-4	0.42121705858077413
>5k	4.7692791421321016E-4	0.4277168606553622
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5725	0.42458703811790394	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4567	0.3387055027221777	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03648852799196659	0.0
2	0.0	0.0	0.0	0.11280294934101867	0.0
3	0.0	0.0	0.0	0.1569303358353685	0.0
4	0.0	0.0	0.0	0.246742545994457	0.0
5	0.0	0.0	0.0	0.400409680139487	0.0
6	0.0	0.0	0.0	0.5488111933751073	0.0
7	0.0	0.0	0.0	0.6352860381690769	0.0
8	0.0	0.0	0.0	0.8668250308335478	0.0
9	0.0	0.0	0.0	0.9437327615808432	0.0
10	0.0	0.0	0.0	1.1112685029098117	0.0
11	0.0	0.0	0.0	1.2575934332515804	0.0
12	0.0	0.0	0.0	1.4075523836575894	0.0
13	0.0	0.0	0.0	1.4582803372073965	0.0
14	0.0	0.0	0.0	1.475337982406893	0.0
15	7.41636747804199E-5	0.0	0.0	1.5071541988876933	0.0
16	7.41636747804199E-5	0.0	0.0	1.5793896181238223	0.0
17	7.41636747804199E-5	0.0	0.0	1.6678668821368632	0.0
18	7.41636747804199E-5	0.0	0.0	1.8044763710823966	0.0
19	1.483273495608398E-4	0.0	0.0	1.861804891687661	0.0
20	1.483273495608398E-4	0.0	0.0	1.926772270795309	0.0
21	1.483273495608398E-4	0.0	0.0	2.011541351069329	0.0
22	1.483273495608398E-4	0.0	0.0	2.1008344155049543	0.0
23	2.224910243412597E-4	0.0	0.0	2.2111899635782195	0.0
24	2.224910243412597E-4	0.0	0.0	2.2912125686662925	0.0
25	2.224910243412597E-4	0.0	0.0	2.351878454636676	0.0
26	2.224910243412597E-4	0.0	0.0	2.42151814525549	0.0
27	2.224910243412597E-4	0.0	0.0	2.480774921405046	0.0
28	2.224910243412597E-4	0.0	0.0	2.5483380291300084	0.0
29	2.224910243412597E-4	0.0	0.0	2.621982558186965	0.0
30	2.966546991216796E-4	0.0	0.0	2.7290749045698917	0.0
31	3.708183739020995E-4	0.0	0.0	2.8264518095565827	0.0
32	3.708183739020995E-4	0.0	0.0	2.900467156987442	0.0
33	3.708183739020995E-4	0.0	0.0	2.984420436838877	0.0
34	3.708183739020995E-4	0.0	0.0	3.077495848688304	0.0
35	3.708183739020995E-4	0.0	0.0	3.2063181517818937	0.0
36	3.708183739020995E-4	0.0	0.0	3.3069582584589234	0.0
37	3.708183739020995E-4	0.0	0.0	3.4019619258526412	0.0
38	3.708183739020995E-4	0.0	0.0	3.4957789744498724	0.0
39	3.708183739020995E-4	0.0	0.0	3.603983775954505	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGTA	35	1.2121382E-7	45.000004	41
CTCCGAT	25	3.8911654E-5	45.000004	20
CCGATCA	35	1.2121382E-7	45.000004	11
TGCGGAT	25	3.8911654E-5	45.000004	44
ACGGGTA	65	0.0	45.000004	5
TTATCCG	50	2.1827873E-11	45.000004	1
ACCTCGT	25	3.8911654E-5	45.000004	28
TACGCGC	35	1.2121382E-7	45.000004	34
TGCGATA	25	3.8911654E-5	45.000004	1
TGTTACG	45	3.8562575E-10	45.000004	1
ATGCGCA	25	3.8911654E-5	45.000004	12
TCGAGTA	25	3.8911654E-5	45.000004	12
CGGTACC	35	1.2121382E-7	45.000004	23
TATACGT	25	3.8911654E-5	45.000004	21
GCTAACG	45	3.8562575E-10	45.000004	1
TACGTCT	25	3.8911654E-5	45.000004	31
ACAGTCG	20	7.033722E-4	45.0	18
CACGATT	20	7.033722E-4	45.0	18
TCGATTG	20	7.033722E-4	45.0	1
GCGCATC	20	7.033722E-4	45.0	19
>>END_MODULE