FastQCFastQC Report
Thu 26 May 2016
SRR1545542_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545542_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1812230
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66750.3683307306467722No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC36630.20212666162683543No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT28210.1556645679632276No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCTA3000.045.00000431
ACGTTAC207.0343644E-445.019
CGCATTA207.0343644E-445.031
TACGGGT800.045.04
ACGGGTA1700.045.05
CCGTAGT253.8916995E-545.029
ACGGCAC207.0343644E-445.05
CCGAATA207.0343644E-445.025
TATACGC207.0343644E-445.016
CGACGGT3050.043.5245928
ACGTAAG1400.043.3928571
CTATGCG750.042.0000041
GTACGGG6300.041.071433
CGTAAGG2250.041.02
TATAGCG1100.040.9090921
CGTTTTT55900.040.8542061
TACGCCC451.929402E-840.041
ATAGCGG2650.039.9056552
ACCGTTA403.4596997E-739.37526
CACCGTA403.4596997E-739.37524
Produced by FastQC (version 0.11.5)