##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545542_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1812230
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.28744530219674	31.0	31.0	33.0	30.0	34.0
2	31.635853065008305	31.0	31.0	34.0	30.0	34.0
3	31.737176296606943	31.0	31.0	34.0	30.0	34.0
4	35.45974793486478	37.0	35.0	37.0	33.0	37.0
5	35.31496002163081	37.0	35.0	37.0	33.0	37.0
6	35.39911545444011	37.0	35.0	37.0	33.0	37.0
7	35.82954205592005	37.0	35.0	37.0	35.0	37.0
8	35.84710053359673	37.0	35.0	37.0	35.0	37.0
9	37.55312018893849	39.0	37.0	39.0	35.0	39.0
10	36.88634775939036	39.0	37.0	39.0	32.0	39.0
11	36.532647621990584	39.0	35.0	39.0	32.0	39.0
12	35.82260860928248	37.0	35.0	39.0	31.0	39.0
13	35.4954707735773	37.0	35.0	39.0	30.0	39.0
14	36.48530981166849	38.0	35.0	41.0	31.0	41.0
15	36.69565728411956	38.0	35.0	41.0	31.0	41.0
16	36.84562445164245	38.0	35.0	41.0	32.0	41.0
17	36.78879446869327	38.0	35.0	41.0	32.0	41.0
18	36.72739442565237	38.0	35.0	40.0	31.0	41.0
19	36.670497674136286	38.0	35.0	40.0	31.0	41.0
20	36.51666731044073	38.0	35.0	40.0	31.0	41.0
21	36.33594632027943	38.0	35.0	40.0	31.0	41.0
22	36.25676652522031	38.0	35.0	40.0	30.0	41.0
23	36.1998708773169	38.0	35.0	40.0	30.0	41.0
24	36.14353752007196	38.0	35.0	40.0	30.0	41.0
25	36.03271714958918	37.0	34.0	40.0	30.0	41.0
26	35.91770360274358	37.0	34.0	40.0	30.0	41.0
27	35.86942109997075	37.0	34.0	40.0	30.0	41.0
28	35.85018237199473	37.0	34.0	40.0	30.0	41.0
29	35.8306434613708	37.0	34.0	40.0	30.0	41.0
30	35.75390651297021	37.0	34.0	40.0	30.0	41.0
31	35.59086539787996	37.0	34.0	40.0	29.0	41.0
32	35.42471816491284	37.0	34.0	40.0	29.0	41.0
33	35.25236862870607	37.0	34.0	40.0	27.0	41.0
34	35.09054590201023	37.0	34.0	40.0	27.0	41.0
35	34.951315230406735	37.0	34.0	40.0	26.0	41.0
36	34.770825998907426	37.0	33.0	40.0	25.0	41.0
37	34.70155278303527	36.0	33.0	40.0	25.0	41.0
38	34.700768114422566	36.0	33.0	40.0	26.0	41.0
39	34.640333180666914	36.0	33.0	40.0	25.0	41.0
40	34.483725023865624	36.0	33.0	40.0	24.0	41.0
41	34.42522748216286	36.0	33.0	40.0	24.0	41.0
42	34.334873056951935	36.0	33.0	40.0	24.0	41.0
43	34.268027237160844	36.0	33.0	40.0	24.0	41.0
44	34.20296595906701	35.0	33.0	40.0	24.0	41.0
45	34.159959276692256	35.0	33.0	40.0	24.0	41.0
46	34.10852982237354	35.0	33.0	40.0	24.0	41.0
47	34.03495306887095	35.0	33.0	40.0	23.0	41.0
48	33.99940625638026	35.0	33.0	40.0	23.0	41.0
49	33.92456034830016	35.0	33.0	39.0	24.0	41.0
50	33.81561280852872	35.0	33.0	39.0	24.0	41.0
51	33.659586255607735	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	3.0
13	6.0
14	25.0
15	55.0
16	148.0
17	480.0
18	1057.0
19	2090.0
20	3555.0
21	5619.0
22	8312.0
23	11960.0
24	16717.0
25	22760.0
26	29552.0
27	35654.0
28	39385.0
29	45097.0
30	52935.0
31	63398.0
32	78044.0
33	101078.0
34	165218.0
35	245256.0
36	132121.0
37	163071.0
38	227314.0
39	361120.0
40	200.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.224171324831836	23.291248903284902	26.93510205658222	14.549477715301038
2	31.495891801813237	25.701870071679643	29.350744662653195	13.451493463853925
3	28.98296573834447	25.430436533994026	31.728809257103123	13.857788470558372
4	26.020703773803543	28.070112513312328	30.33224259613846	15.576941116745665
5	22.80383836488746	33.06782251700943	29.89471535070052	14.233623767402593
6	20.89624385425691	42.53251518846945	26.272051560784227	10.299189396489409
7	87.12404054672972	4.443530898395899	6.7267951639692525	1.7056333909051278
8	87.90782626929253	3.6111310374510963	6.743570076645901	1.7374726166104744
9	84.13336055577935	4.9515238132025186	8.080762375636647	2.834353255381491
10	48.087163329158	25.532410345265227	14.581758386076823	11.798667939499953
11	39.78932034013343	22.233270611346242	21.58925743421089	16.388151614309443
12	37.9005424256303	20.597937347908378	26.36525165128047	15.136268575180855
13	23.11301545609553	35.391423825894066	24.92398867693394	16.57157204107646
14	17.27473885765052	37.2303736280715	27.687600359777733	17.807287154500255
15	16.707316400236174	23.9149004265463	42.13780811486401	17.239975058353522
16	18.017801272465416	19.40912577321863	41.47255039371382	21.10052256060213
17	17.764356621400154	19.863152028164198	29.879264773235185	32.49322657720047
18	21.58870562787284	23.221610943423297	33.54083090998383	21.64885251872003
19	27.74079449076552	25.292043504411694	25.46404153998113	21.50312046484166
20	30.542591172202204	22.269469107122163	27.062569320671216	20.125370400004414
21	23.36171457265358	26.465514862903717	27.984748072816362	22.188022491626338
22	23.195344961732232	23.759677303653508	25.706063799848806	27.33891393476545
23	20.661063992981024	28.437008547480175	25.725652924849495	25.17627453468931
24	20.4300778598743	23.675913101537883	37.20968089039471	18.68432814819311
25	19.631669269353228	26.062806597396577	32.73635244974424	21.569171683505957
26	19.778173852104867	30.63170789579689	26.476054363960422	23.11406388813782
27	18.95570650524492	31.669821159565835	27.920904079504254	21.453568255684985
28	17.71563212175055	27.987176020703775	34.07696594803088	20.220225909514795
29	17.925649614011466	23.999823421971826	34.505278027623426	23.569248936393283
30	19.6093211126623	29.387494964767164	31.035188690177296	19.96799523239324
31	26.973066332639895	27.94959800908273	25.22549566004315	19.85183999823422
32	28.597970456288664	25.236255883635078	25.99410670830965	20.17166695176661
33	26.91385751256739	27.242182283705713	24.810647655098965	21.033312548627933
34	21.244378472931142	26.159483067822514	27.651788128438444	24.9443503308079
35	21.534738968011787	25.550012967448943	29.700313977806346	23.214934086732917
36	28.248235599234096	27.602401461183184	25.068120492431976	19.08124244715075
37	21.21386358243711	32.20220391451416	27.512015583010985	19.071916920037744
38	21.55879772435066	30.831075525733485	25.883800621333936	21.726326128581913
39	21.210718286310236	31.325328462722723	26.418114698465427	21.045838552501614
40	24.31589809240549	26.852882912213126	26.36392731606915	22.46729167931223
41	18.920059815807043	25.946541001969948	27.87455234710826	27.258846835114745
42	21.37002477610458	26.545857865723445	24.866269733974164	27.21784762419781
43	21.92199665605359	25.870446907953188	26.39940846360561	25.808147972387612
44	19.875015864432218	28.925688240455127	28.225721900641748	22.9735739944709
45	18.58649288445727	35.013657206866675	23.838640790628123	22.56120911804793
46	21.429343957444697	32.33314755853286	26.031353636127864	20.206154847894584
47	21.375653200752666	27.999150218239404	27.63319225484624	22.99200432616169
48	22.23106338599405	25.115520656870267	30.0150643130287	22.638351644106987
49	20.758788895449253	25.904493359010722	31.18086556342186	22.155852182118164
50	20.05402184049486	31.283446361664915	28.49185809748211	20.170673700358122
51	19.224381011240297	30.986850454964326	26.481737969242314	23.307030564553063
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1294.0
1	2479.0
2	3664.0
3	8463.5
4	13263.0
5	10174.5
6	7086.0
7	7280.5
8	7475.0
9	7537.5
10	7600.0
11	7888.5
12	8177.0
13	7917.5
14	7658.0
15	7419.0
16	7180.0
17	6702.0
18	6224.0
19	6553.0
20	6882.0
21	7011.5
22	7141.0
23	6969.5
24	6798.0
25	8748.5
26	13622.5
27	16546.0
28	19883.5
29	23221.0
30	25144.5
31	27068.0
32	34088.0
33	41108.0
34	49985.5
35	58863.0
36	61148.0
37	63433.0
38	72127.0
39	80821.0
40	94393.0
41	107965.0
42	127774.5
43	147584.0
44	149146.0
45	150708.0
46	151987.0
47	153266.0
48	145674.5
49	138083.0
50	129587.5
51	121092.0
52	114445.5
53	107799.0
54	97024.0
55	86249.0
56	81413.5
57	76578.0
58	71829.0
59	67080.0
60	66091.5
61	65103.0
62	56693.5
63	48284.0
64	43793.0
65	39302.0
66	34250.0
67	29198.0
68	25551.5
69	21905.0
70	18566.0
71	15227.0
72	12434.0
73	9641.0
74	8043.0
75	5189.5
76	3934.0
77	3119.5
78	2305.0
79	1776.5
80	1248.0
81	914.0
82	580.0
83	445.5
84	311.0
85	199.5
86	88.0
87	50.5
88	13.0
89	13.5
90	14.0
91	14.0
92	14.0
93	9.0
94	4.0
95	6.0
96	8.0
97	4.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1812230.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.736766736083258
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.92483946022774	13.376833944647345
2	6.543642314344904	2.1903883003910933
3	2.354982959781686	1.1824440139595107
4	1.2580310075666616	0.8422148608161203
5	0.7790683231267206	0.6519542397821732
6	0.5364995585553679	0.5387560779331702
7	0.4235871505197488	0.49626355314558573
8	0.3411601441305009	0.456793420156859
9	0.2854372939919596	0.42995676665900223
>10	4.247334028657117	17.879862639996112
>50	1.8525745376026608	22.374629105630344
>100	1.4442111548224708	37.97892123016812
>500	0.007304056415193497	0.792989446566132
>1k	9.960076929809315E-4	0.4370860476973719
>5k	3.3200256432697715E-4	0.37090635245104975
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6675	0.3683307306467722	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3663	0.20212666162683543	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	2821	0.1556645679632276	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.014126242254018529	0.0
2	0.0	0.0	0.0	0.05170425387506001	0.0
3	0.0	0.0	0.0	0.07686662289003052	0.0
4	0.0	0.0	0.0	0.12757762535660486	0.0
5	0.0	0.0	0.0	0.2205018126838205	0.0
6	0.0	0.0	0.0	0.3185577989548788	0.0
7	0.0	0.0	0.0	0.38560226902766204	0.0
8	0.0	0.0	0.0	0.5461227327657086	0.0
9	0.0	0.0	0.0	0.6034002306550493	0.0
10	0.0	0.0	0.0	0.7139270401659833	0.0
11	0.0	0.0	0.0	0.8284268553108601	0.0
12	5.518063380475988E-5	0.0	0.0	0.9327182532018563	0.0
13	5.518063380475988E-5	0.0	0.0	0.9692478327806073	0.0
14	5.518063380475988E-5	0.0	0.0	0.9824360042599449	0.0
15	5.518063380475988E-5	0.0	0.0	1.0072672894720869	0.0
16	5.518063380475988E-5	0.0	0.0	1.0616202137697754	0.0
17	5.518063380475988E-5	0.0	0.0	1.1295475739834349	0.0
18	5.518063380475988E-5	0.0	0.0	1.2250100704656695	0.0
19	5.518063380475988E-5	0.0	0.0	1.2694856613123058	0.0
20	5.518063380475988E-5	0.0	0.0	1.3179894384266897	0.0
21	5.518063380475988E-5	0.0	0.0	1.3884551077953682	0.0
22	5.518063380475988E-5	0.0	0.0	1.464052576107889	0.0
23	5.518063380475988E-5	0.0	0.0	1.5568112215336904	0.0
24	5.518063380475988E-5	0.0	0.0	1.6254007493530072	0.0
25	5.518063380475988E-5	0.0	0.0	1.6827334278761525	0.0
26	5.518063380475988E-5	0.0	0.0	1.7408386352725647	0.0
27	5.518063380475988E-5	0.002814212324042754	0.0	1.7999370940774626	0.0
28	1.6554190141427965E-4	0.002814212324042754	0.0	1.8630085585163032	0.0
29	1.6554190141427965E-4	0.002814212324042754	0.0	1.934467479293467	0.0
30	1.6554190141427965E-4	0.002814212324042754	0.0	2.0302058789447255	0.0
31	2.2072253521903952E-4	0.002814212324042754	0.0	2.1112110493701133	0.0
32	2.2072253521903952E-4	0.002814212324042754	0.0	2.185208279302296	0.0
33	2.2072253521903952E-4	0.002814212324042754	0.0	2.266047907826269	0.0
34	2.2072253521903952E-4	0.002814212324042754	0.0	2.34396296275859	0.0
35	2.2072253521903952E-4	0.002814212324042754	0.0	2.459566390579562	0.0
36	2.2072253521903952E-4	0.002814212324042754	0.0	2.549400462413711	0.0
37	2.2072253521903952E-4	0.002814212324042754	0.0	2.6354822511491367	0.0
38	2.2072253521903952E-4	0.002814212324042754	0.0	2.7316069152370286	0.0
39	2.2072253521903952E-4	0.002814212324042754	0.0	2.8276212180573106	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTCTA	300	0.0	45.000004	31
ACGTTAC	20	7.0343644E-4	45.0	19
CGCATTA	20	7.0343644E-4	45.0	31
TACGGGT	80	0.0	45.0	4
ACGGGTA	170	0.0	45.0	5
CCGTAGT	25	3.8916995E-5	45.0	29
ACGGCAC	20	7.0343644E-4	45.0	5
CCGAATA	20	7.0343644E-4	45.0	25
TATACGC	20	7.0343644E-4	45.0	16
CGACGGT	305	0.0	43.52459	28
ACGTAAG	140	0.0	43.392857	1
CTATGCG	75	0.0	42.000004	1
GTACGGG	630	0.0	41.07143	3
CGTAAGG	225	0.0	41.0	2
TATAGCG	110	0.0	40.909092	1
CGTTTTT	5590	0.0	40.854206	1
TACGCCC	45	1.929402E-8	40.0	41
ATAGCGG	265	0.0	39.905655	2
ACCGTTA	40	3.4596997E-7	39.375	26
CACCGTA	40	3.4596997E-7	39.375	24
>>END_MODULE