FastQCFastQC Report
Thu 26 May 2016
SRR1545541_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545541_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2305166
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98660.4279952072865902No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC54750.23751001012508427No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT34010.14753818163203866No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG25710.11153209790531354No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTACGC302.1664564E-645.00000410
GATATCG453.8562575E-1045.00000413
GACGTAT207.0347666E-445.09
TAACGCG207.0347666E-445.01
CCGTAGA207.0347666E-445.022
CCGCGAA351.2126475E-745.018
ATCGGCT406.8193913E-945.045
CGTAAGG2750.044.181822
CGGTCTA2250.044.031
GCTACGA4650.042.0967710
TATTACG750.042.0000041
TACGGGA5750.041.478264
CATACGA8350.040.149718
CGGTAGT5700.039.8684212
TAGGGCG8100.039.722225
TATTTCG403.4602454E-739.3758
CTATGCG403.4602454E-739.3751
TCGCATA403.4602454E-739.37528
TATGCCA8400.039.37510
ACGGTCC403.4602454E-739.37541
Produced by FastQC (version 0.11.5)