FastQCFastQC Report
Thu 26 May 2016
SRR1545539_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545539_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences905258
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC48300.5335495516195383No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40810.450810708107523No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCATCG207.032491E-445.043
CGGGTAT207.032491E-445.06
CTCACGA1600.045.024
GGTACGA207.032491E-445.01
CGTATGG253.890147E-545.02
CTACTAT253.890147E-545.045
CGAGTGT207.032491E-445.031
TCTCTAG253.890147E-545.024
CGAAACC253.890147E-545.029
ACGTTAC207.032491E-445.026
GCAACGA253.890147E-545.032
CTATATG253.890147E-545.01
CATGACG207.032491E-445.01
CTATAGC453.8380676E-1045.025
CGCATGT207.032491E-445.038
TCTTACG253.890147E-545.01
ACATTCG207.032491E-445.016
CAACCCG253.890147E-545.023
ATTCGCA253.890147E-545.018
TTACTCG351.2115379E-745.01