##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545539_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	905258
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.471245766400298	31.0	31.0	33.0	30.0	34.0
2	31.8204522909491	31.0	31.0	34.0	30.0	34.0
3	31.878887565754734	31.0	31.0	34.0	30.0	34.0
4	35.56580665401466	37.0	35.0	37.0	33.0	37.0
5	35.44753871272057	37.0	35.0	37.0	33.0	37.0
6	35.532769663455056	37.0	35.0	37.0	33.0	37.0
7	35.965473931188676	37.0	35.0	37.0	35.0	37.0
8	36.00744980988845	37.0	35.0	37.0	35.0	37.0
9	37.76109020853723	39.0	38.0	39.0	35.0	39.0
10	37.17090045047931	39.0	37.0	39.0	33.0	39.0
11	36.70391866186214	39.0	35.0	39.0	32.0	39.0
12	35.400123500703664	35.0	35.0	39.0	31.0	39.0
13	34.84098787307044	35.0	34.0	39.0	30.0	39.0
14	35.70007997719987	36.0	35.0	40.0	30.0	41.0
15	36.077820908514475	36.0	35.0	40.0	31.0	41.0
16	36.34080781390499	36.0	35.0	40.0	32.0	41.0
17	36.289814616385605	36.0	35.0	40.0	32.0	41.0
18	36.25848653091163	36.0	35.0	40.0	31.0	41.0
19	36.185109659345734	36.0	35.0	40.0	31.0	41.0
20	35.97926447487899	36.0	35.0	40.0	31.0	41.0
21	35.741148932127636	35.0	34.0	40.0	31.0	41.0
22	35.641007315041676	35.0	34.0	40.0	30.0	41.0
23	35.67813706147861	35.0	34.0	40.0	31.0	41.0
24	35.627663052963904	35.0	34.0	40.0	31.0	41.0
25	35.52227983624558	35.0	34.0	40.0	31.0	41.0
26	35.40687848105181	35.0	34.0	40.0	30.0	41.0
27	35.336037903006655	35.0	34.0	40.0	30.0	41.0
28	35.3996396607376	35.0	34.0	40.0	30.0	41.0
29	35.45658806660643	36.0	34.0	40.0	30.0	41.0
30	35.40488015571251	36.0	34.0	40.0	30.0	41.0
31	35.11553833271841	35.0	34.0	40.0	30.0	41.0
32	34.876835112200055	35.0	34.0	40.0	29.0	41.0
33	34.64770485320207	35.0	34.0	40.0	28.0	41.0
34	34.43894558236436	35.0	34.0	40.0	27.0	41.0
35	34.275574477110396	35.0	33.0	40.0	26.0	41.0
36	34.06560229238515	35.0	33.0	40.0	24.0	41.0
37	33.9714423954276	35.0	33.0	39.0	24.0	41.0
38	34.010368314889234	35.0	33.0	39.0	25.0	41.0
39	34.00217838450475	35.0	33.0	39.0	24.0	41.0
40	33.79359254488776	35.0	33.0	39.0	23.0	41.0
41	33.840969093893676	35.0	33.0	39.0	23.0	41.0
42	33.80347149652364	35.0	33.0	39.0	23.0	41.0
43	33.677950374368415	35.0	33.0	39.0	23.0	41.0
44	33.59133197386822	35.0	33.0	39.0	23.0	41.0
45	33.54105569903829	35.0	33.0	39.0	23.0	41.0
46	33.52780422818688	35.0	33.0	39.0	23.0	41.0
47	33.48859441175886	35.0	33.0	39.0	23.0	41.0
48	33.498755051046224	35.0	33.0	38.0	23.0	41.0
49	33.49152727730658	35.0	33.0	38.0	24.0	41.0
50	33.33661784817146	35.0	33.0	38.0	24.0	40.0
51	33.13445448700812	35.0	33.0	38.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	2.0
14	3.0
15	20.0
16	56.0
17	221.0
18	504.0
19	912.0
20	1638.0
21	2631.0
22	4056.0
23	5753.0
24	8706.0
25	12377.0
26	16388.0
27	19112.0
28	20473.0
29	22883.0
30	26861.0
31	32830.0
32	41418.0
33	55177.0
34	104556.0
35	180740.0
36	53953.0
37	62703.0
38	86625.0
39	144583.0
40	76.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.859646642172727	23.761292360851822	29.06961330361068	15.309447693364763
2	30.207300018337314	25.873286952448915	30.143340351590375	13.776072677623397
3	27.975560558426437	24.583378440179484	33.47288839203851	13.968172609355564
4	24.13146307461519	28.196492049780286	32.51625503447636	15.155789841128165
5	22.064869904491317	33.36739360491705	30.634139659632943	13.933596830958688
6	20.73453092930413	41.032722163184424	27.264603019249762	10.96814388826169
7	87.14797328496407	4.436193880639553	7.075551942098275	1.3402808922981073
8	89.43825959008372	2.724416685629953	6.71852665207046	1.1187970722158764
9	86.53996982075827	3.7057943702237375	7.844835394992367	1.909400414025615
10	61.00360339262399	17.741792947424933	12.10870271237592	9.145900947575166
11	54.03520322383232	17.245691283589874	17.202499177030194	11.516606315547612
12	48.3255602270292	18.51162872904741	22.096573573500596	11.066237470422797
13	20.008108185732688	47.0101341275084	21.32773198358921	11.654025703169705
14	12.97530648721138	48.01117471483268	26.6004829562401	12.413035841715843
15	11.668938578836089	20.833176840193623	55.660375274231214	11.837509306739074
16	13.863892945436548	14.05842312357361	53.38323439284712	18.694449538142717
17	13.703165285476626	15.064986998181734	29.386208130720743	41.845639585620894
18	20.2847144129077	19.926915862660145	38.8629539865983	20.925415737833855
19	29.51821469680467	21.792461375652024	26.93707208331768	21.752251844225622
20	33.39169606896598	20.635332689686255	26.106038278590194	19.866932962757577
21	21.51762260040784	26.42694126978165	29.915780915495915	22.139655214314594
22	23.070218655896994	22.789414730386255	24.836676395016667	29.303690218700083
23	17.600175861467118	29.105956533938393	24.42287171171092	28.870995892883577
24	19.16790572411401	20.672559645979376	44.40303206378734	15.756502566119272
25	16.123248841766657	22.744897034878456	40.46824220277534	20.663611920579548
26	15.739822238522056	34.70259307291402	27.524418453081882	22.033166235482042
27	15.03670776728844	37.92366375110742	28.274370400482514	18.765258081121626
28	12.624467278941474	29.864966672484528	40.533196061233376	16.977369987340627
29	12.882404795097088	22.407645113326808	40.78240678348051	23.927543308095593
30	17.191783999699535	31.597290496190038	33.42306834073822	17.78785716337221
31	30.49760399797627	27.019148132355635	24.695280240550208	17.787967629117887
32	32.825448656626065	24.593430823036087	26.97617695728731	15.604943563050533
33	31.491464311831546	27.881222811618343	23.03984057583584	17.58747230071427
34	19.281464510669885	28.67624478325516	26.746076809042286	25.296213897032672
35	19.05523066352355	26.31791157879853	31.590331154212393	23.036526603465532
36	34.88000106047116	23.14003300716481	25.4056854509985	16.574280481365534
37	21.153748434148056	32.43362665671002	29.806309361530083	16.606315547611842
38	20.787664952974733	33.88437329468505	22.416371907235284	22.91158984510493
39	20.40191856907092	32.53359815654764	27.680617017469057	19.383866256912395
40	24.647006709689393	25.516813991149483	24.473575488976625	25.3626038101845
41	16.401953918109534	23.237905657834563	29.140200915098234	31.219939508957665
42	21.931979612441978	23.4210578641669	25.301074389842455	29.34588813354867
43	22.60327994892064	23.32307474775147	27.723256795300344	26.350388508027546
44	18.951282396841563	29.734396161094406	29.057683003077578	22.256638438986453
45	15.940648964162705	41.43293955977191	21.450128029799238	21.176283446266147
46	23.214818316988083	33.62301134041345	25.36470265935236	17.7974676832461
47	21.789478800518747	28.431563156580776	25.597674916984992	24.181283125915485
48	23.11550961162453	24.978956275448546	31.001438264008712	20.904095848918207
49	23.015206714549883	23.113189830965318	33.307963033742865	20.563640420741933
50	20.808763910399026	34.68624414255383	25.521895415450622	18.983096531596516
51	18.10710316837852	35.92335002838969	22.896787435184223	23.072759368047564
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1062.0
1	1384.5
2	1707.0
3	4625.5
4	7544.0
5	5664.5
6	3785.0
7	4124.5
8	4464.0
9	4967.5
10	5471.0
11	5575.5
12	5680.0
13	5613.5
14	5547.0
15	5069.5
16	4592.0
17	4379.0
18	4166.0
19	3839.0
20	3512.0
21	3310.5
22	3109.0
23	3390.0
24	3671.0
25	3621.5
26	4381.5
27	5191.0
28	5886.5
29	6582.0
30	8022.0
31	9462.0
32	11159.5
33	12857.0
34	15793.5
35	18730.0
36	22371.5
37	26013.0
38	27993.5
39	29974.0
40	48085.5
41	66197.0
42	83699.0
43	101201.0
44	101919.5
45	102638.0
46	97540.0
47	92442.0
48	82145.0
49	71848.0
50	67003.5
51	62159.0
52	57399.5
53	52640.0
54	47200.0
55	41760.0
56	37943.0
57	34126.0
58	32024.0
59	29922.0
60	26945.5
61	23969.0
62	22233.5
63	20498.0
64	17440.0
65	14382.0
66	11641.0
67	8900.0
68	7289.5
69	5679.0
70	4875.5
71	4072.0
72	3508.0
73	2944.0
74	2270.0
75	1126.5
76	657.0
77	580.0
78	503.0
79	377.0
80	251.0
81	179.5
82	108.0
83	84.5
84	61.0
85	33.5
86	6.0
87	3.0
88	0.0
89	0.5
90	1.0
91	1.5
92	2.0
93	1.5
94	1.0
95	2.5
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	905258.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.139242710674008
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.59485639521435	13.168791913049557
2	6.388976152555957	2.0622647359761763
3	2.008484926917083	0.9724627714873542
4	0.9667963687680396	0.6241344498938274
5	0.5977209004782364	0.4823381343030442
6	0.43781826513451927	0.42396331465033377
7	0.3569130548648022	0.40322144933498055
8	0.2642604694224866	0.3411971083876927
9	0.21902323728517265	0.31813822672406517
>10	3.6373313425627267	14.854279260947036
>50	1.8915405243658094	21.96571398138214
>100	1.629434872834657	42.8447228140401
>500	0.00547479167689091	0.5545631214294249
>1k	0.0013686979192227275	0.984208718394265
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4830	0.5335495516195383	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4081	0.450810708107523	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.021319888915646148	0.0
2	0.0	0.0	0.0	0.07699462473681536	0.0
3	0.0	0.0	0.0	0.10262267773386151	0.0
4	0.0	0.0	0.0	0.1485764279354615	0.0
5	0.0	0.0	0.0	0.24048392833866147	0.0
6	0.0	0.0	0.0	0.32852512764316916	0.0
7	0.0	0.0	0.0	0.3825428772791845	0.0
8	0.0	0.0	0.0	0.5030610058127075	0.0
9	0.0	0.0	0.0	0.5450379891699383	0.0
10	0.0	0.0	0.0	0.633079188474446	0.0
11	0.0	0.0	0.0	0.7672950694719075	0.0
12	0.0	0.0	0.0	0.8564409262331844	0.0
13	0.0	0.0	0.0	0.8975341836249997	0.0
14	0.0	0.0	0.0	0.9134412510024766	0.0
15	1.1046574567692305E-4	0.0	0.0	0.9407262901846767	0.0
16	1.1046574567692305E-4	0.0	0.0	1.0007091900872458	0.0
17	1.1046574567692305E-4	0.0	0.0	1.0772619518413535	0.0
18	1.1046574567692305E-4	0.0	0.0	1.1780067118987074	0.0
19	1.1046574567692305E-4	0.0	0.0	1.227495365961969	0.0
20	1.1046574567692305E-4	0.0	0.0	1.274443307874661	0.0
21	1.1046574567692305E-4	0.0	0.0	1.3382925088759228	0.0
22	1.1046574567692305E-4	0.0	0.0	1.400816120929061	0.0
23	1.1046574567692305E-4	0.0	0.0	1.4855433478632611	0.0
24	1.1046574567692305E-4	0.0	0.0	1.5491716173731687	0.0
25	1.1046574567692305E-4	0.0	0.0	1.596119559285861	0.0
26	1.1046574567692305E-4	0.0	0.0	1.649474514447815	0.0
27	1.1046574567692305E-4	0.0	0.0	1.6998468944764917	0.0
28	1.1046574567692305E-4	0.0	0.0	1.7547483700779225	0.0
29	1.1046574567692305E-4	0.0	0.0	1.8136266125237226	0.0
30	1.1046574567692305E-4	0.0	0.0	1.8879700593642916	0.0
31	1.1046574567692305E-4	0.0	0.0	1.9588890680888764	0.0
32	1.1046574567692305E-4	0.0	0.0	2.0271568989172146	0.0
33	1.1046574567692305E-4	0.0	0.0	2.0906747026814454	0.0
34	1.1046574567692305E-4	0.0	0.0	2.1640239578109224	0.0
35	1.1046574567692305E-4	0.0	0.0	2.2762571554186763	0.0
36	1.1046574567692305E-4	0.0	0.0	2.3532517801554915	0.0
37	1.1046574567692305E-4	0.0	0.0	2.4322347883144917	0.0
38	1.1046574567692305E-4	0.0	0.0	2.509229413051307	0.0
39	1.1046574567692305E-4	0.0	0.0	2.5856717090597376	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCATCG	20	7.032491E-4	45.0	43
CGGGTAT	20	7.032491E-4	45.0	6
CTCACGA	160	0.0	45.0	24
GGTACGA	20	7.032491E-4	45.0	1
CGTATGG	25	3.890147E-5	45.0	2
CTACTAT	25	3.890147E-5	45.0	45
CGAGTGT	20	7.032491E-4	45.0	31
TCTCTAG	25	3.890147E-5	45.0	24
CGAAACC	25	3.890147E-5	45.0	29
ACGTTAC	20	7.032491E-4	45.0	26
GCAACGA	25	3.890147E-5	45.0	32
CTATATG	25	3.890147E-5	45.0	1
CATGACG	20	7.032491E-4	45.0	1
CTATAGC	45	3.8380676E-10	45.0	25
CGCATGT	20	7.032491E-4	45.0	38
TCTTACG	25	3.890147E-5	45.0	1
ACATTCG	20	7.032491E-4	45.0	16
CAACCCG	25	3.890147E-5	45.0	23
ATTCGCA	25	3.890147E-5	45.0	18
TTACTCG	35	1.2115379E-7	45.0	1
>>END_MODULE