FastQCFastQC Report
Thu 26 May 2016
SRR1545538_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545538_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1563133
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53140.3399582761031851No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC39150.25045853423860925No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG25050.16025507746301818No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGGC207.034067E-445.03
CAAACCG207.034067E-445.010
TCGATAG453.8562575E-1045.01
CCCGTAA207.034067E-445.039
CCGTAAC207.034067E-445.040
GGTATAC253.891452E-545.08
CATACGT406.8175723E-945.039
CATACGC207.034067E-445.020
CGGTCTA1100.045.031
TATACGC207.034067E-445.01
GCGTATT207.034067E-445.031
TGACGTA302.1660053E-644.99999626
CTCACGA1150.043.0434824
AGTACGG1650.042.2727282
ATGTACG650.041.538461
TCACGAC1200.041.24999625
TAATGCG556.184564E-1140.9090921
TCGAACG556.184564E-1140.9090921
ATGGGCG4300.040.8139535
GGCGATA6250.040.328
Produced by FastQC (version 0.11.5)