##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545538_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1563133
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.31985314109548	31.0	31.0	33.0	30.0	34.0
2	31.665718144265394	31.0	31.0	34.0	30.0	34.0
3	31.780290608668615	31.0	31.0	34.0	30.0	34.0
4	35.49951731554513	37.0	35.0	37.0	33.0	37.0
5	35.36754837880078	37.0	35.0	37.0	33.0	37.0
6	35.432549245649604	37.0	35.0	37.0	33.0	37.0
7	35.82651828091404	37.0	35.0	37.0	35.0	37.0
8	35.83971037653226	37.0	35.0	37.0	35.0	37.0
9	37.52253391106195	39.0	37.0	39.0	35.0	39.0
10	36.92727298316906	39.0	37.0	39.0	32.0	39.0
11	36.56449643120579	39.0	35.0	39.0	32.0	39.0
12	35.798352411471065	37.0	35.0	39.0	31.0	39.0
13	35.46585799161044	37.0	35.0	39.0	30.0	39.0
14	36.454026624733785	38.0	35.0	41.0	31.0	41.0
15	36.66618771403329	38.0	35.0	41.0	31.0	41.0
16	36.84214778908768	38.0	35.0	41.0	32.0	41.0
17	36.78891559451435	38.0	35.0	41.0	32.0	41.0
18	36.7223531203039	38.0	35.0	40.0	31.0	41.0
19	36.666254886820255	38.0	35.0	40.0	31.0	41.0
20	36.519090186183774	38.0	35.0	40.0	31.0	41.0
21	36.338898225550864	38.0	35.0	40.0	31.0	41.0
22	36.271192534480434	38.0	35.0	40.0	31.0	41.0
23	36.20528195617391	37.0	35.0	40.0	31.0	41.0
24	36.14761955636533	37.0	35.0	40.0	31.0	41.0
25	36.03388771141035	37.0	35.0	40.0	30.0	41.0
26	35.943923517704505	37.0	34.0	40.0	30.0	41.0
27	35.88759305830022	37.0	34.0	40.0	30.0	41.0
28	35.864784378552564	37.0	35.0	40.0	30.0	41.0
29	35.867248020481945	37.0	35.0	40.0	30.0	41.0
30	35.765053261622654	37.0	34.0	40.0	30.0	41.0
31	35.61040295355546	37.0	34.0	40.0	29.0	41.0
32	35.44428273217954	37.0	34.0	40.0	29.0	41.0
33	35.299910500258136	37.0	34.0	40.0	29.0	41.0
34	35.142941771429555	37.0	34.0	40.0	27.0	41.0
35	34.99682176756552	36.0	34.0	40.0	27.0	41.0
36	34.83202005203652	36.0	34.0	40.0	26.0	41.0
37	34.770668906612556	36.0	33.0	40.0	26.0	41.0
38	34.74968988563353	36.0	33.0	40.0	26.0	41.0
39	34.70375841339157	36.0	34.0	40.0	26.0	41.0
40	34.51725860819265	36.0	33.0	40.0	24.0	41.0
41	34.48619663202044	36.0	33.0	40.0	24.0	41.0
42	34.40920958101454	36.0	33.0	40.0	24.0	41.0
43	34.34138169944592	35.0	33.0	40.0	24.0	41.0
44	34.27987253803739	35.0	33.0	40.0	24.0	41.0
45	34.241408760482955	35.0	33.0	40.0	24.0	41.0
46	34.21297739859628	35.0	33.0	40.0	24.0	41.0
47	34.174362002465564	35.0	33.0	40.0	24.0	41.0
48	34.108374015518834	35.0	33.0	40.0	24.0	41.0
49	34.051794696932376	35.0	33.0	39.0	24.0	41.0
50	33.93226807955561	35.0	33.0	39.0	24.0	41.0
51	33.78621844718268	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	3.0
13	8.0
14	28.0
15	46.0
16	157.0
17	406.0
18	888.0
19	1684.0
20	3029.0
21	4432.0
22	6689.0
23	9664.0
24	13421.0
25	18757.0
26	24754.0
27	29222.0
28	32823.0
29	37812.0
30	43989.0
31	54664.0
32	67969.0
33	87809.0
34	148711.0
35	222549.0
36	111237.0
37	137839.0
38	190344.0
39	314022.0
40	176.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.28221846765439	23.052229080954724	27.96671812315395	13.698834328236945
2	32.20180240580936	25.63531062296043	29.02331407500193	13.13957289622828
3	29.722422852054176	24.971899384121503	31.07003690664838	14.235640857175941
4	26.612002945366775	27.63174982551069	30.053168860231345	15.703078368891193
5	23.7929849859225	32.444967894606535	29.20749545943947	14.554551660031487
6	22.273472570792123	41.23327957377907	26.01493283041174	10.478315025017066
7	86.05736044213768	4.718792322854165	7.202841984655177	2.0210052503529767
8	87.08382460097765	3.7966059190100903	7.040027943879376	2.079541536132882
9	82.56181655687648	5.509639934669667	8.467545627915218	3.460997880538636
10	49.21801279865501	23.536704810147313	14.633367730065197	12.61191466113248
11	41.740977895035165	22.977315429973007	20.42033531375769	14.861371361234138
12	38.96226360776722	21.160131607483176	25.434879821486717	14.442724963262881
13	22.2796140827428	36.767760644807574	23.968657817345036	16.983967455104587
14	17.291234974886972	38.93961678244909	27.236581915934217	16.532566326729714
15	16.038430511031372	24.598930481283425	43.63441882424593	15.728220183439284
16	17.77334366301524	19.4085212198834	43.739912086815394	19.078223030285972
17	18.07088712220905	20.066174791268562	29.11031882763655	32.752619258885844
18	21.801599735914987	23.391995434809452	34.17079672683003	20.635608102445538
19	28.53269683385867	24.138125162734074	27.000453576247192	20.32872442716007
20	30.765840142841334	22.394255639155467	26.0331014699325	20.8068027480707
21	23.07673115467462	27.232999367296323	28.104454323464477	21.58581515456458
22	23.06911823881909	24.229991945662974	25.820579566805897	26.880310248712043
23	20.46479730131729	29.35892211347339	25.617397879770948	24.55888270543837
24	20.019729607141553	23.82446023466973	38.025874957537205	18.129935200651513
25	19.523162776296065	24.777993939095396	34.515489085061866	21.18335419954668
26	18.256411962385798	32.572852086162854	27.4732220482838	21.697513903167547
27	19.259909425493543	32.97038703680365	27.68094589519894	20.088757642503868
28	17.370306941251958	28.617654415843052	35.71647454183361	18.295564101071374
29	18.184441119213783	24.822200030323714	34.60262178586211	22.390737064600387
30	19.723529603686956	30.03877469159694	29.903213610102274	20.33448209461383
31	27.859561534431172	27.426904812322434	24.801984220152733	19.911549433093665
32	28.474096573995944	25.54952137789939	27.164099280099645	18.812282768005026
33	27.7867590281825	27.301771506327356	24.78471121779145	20.126758247698692
34	20.813775923098035	27.172160014534914	27.80460779728916	24.209456265077893
35	21.455052129281384	26.167191147522317	29.766884839613777	22.610871883582522
36	29.339282070047783	26.338449767230298	25.180262971864835	19.14200519085708
37	21.95680086083526	33.056752048610065	27.345273882644662	17.64117320791001
38	21.773451139474375	32.84973191660595	23.84480399300635	21.532012950913327
39	22.700115729115822	30.938634140536987	26.315291149249614	20.045958981097577
40	24.494716700370343	27.016575045117726	26.366406441422452	22.122301813089482
41	19.10515611915301	25.648169413607157	27.869285595019743	27.377388872220088
42	21.9562890681727	26.16629551036284	24.925710096325776	26.95170532513868
43	22.461044581619095	26.156827346105544	26.260401386190424	25.121726686084934
44	19.96835841863744	29.41969749215198	28.36655614077625	22.24538794843433
45	18.68586998035356	35.54662335194766	23.70009461766849	22.067412050030292
46	20.879029487573995	33.30138894131209	26.11249330671159	19.707088264402326
47	22.560844150817623	27.561186412160705	27.40739271706246	22.470576719959208
48	23.10455988070113	24.510966117406515	30.880673621502456	21.503800380389894
49	21.628485867805235	25.7221874274294	31.22811686529553	21.421209839469835
50	19.99957777105339	31.965993936536428	27.52811181134299	20.50631648106719
51	19.332520009493752	31.877197909582872	25.943665702150742	22.846616378772634
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1079.0
1	2604.5
2	4130.0
3	7554.5
4	10979.0
5	8511.0
6	6043.0
7	6283.5
8	6524.0
9	6816.0
10	7108.0
11	6994.0
12	6880.0
13	6661.0
14	6442.0
15	6474.0
16	6506.0
17	6387.0
18	6268.0
19	6016.5
20	5765.0
21	5560.5
22	5356.0
23	6422.5
24	7489.0
25	8056.0
26	11469.0
27	14315.0
28	18213.0
29	22111.0
30	24727.5
31	27344.0
32	31968.5
33	36593.0
34	41626.5
35	46660.0
36	45624.0
37	44588.0
38	52398.0
39	60208.0
40	76583.5
41	92959.0
42	114570.5
43	136182.0
44	141118.5
45	146055.0
46	142734.5
47	139414.0
48	129976.5
49	120539.0
50	113843.5
51	107148.0
52	98883.0
53	90618.0
54	81717.5
55	72817.0
56	70494.0
57	68171.0
58	63492.0
59	58813.0
60	55822.5
61	52832.0
62	46998.5
63	41165.0
64	35411.0
65	29657.0
66	25230.5
67	20804.0
68	18130.5
69	15457.0
70	13842.0
71	12227.0
72	9940.0
73	7653.0
74	6194.5
75	3563.0
76	2390.0
77	1865.0
78	1340.0
79	967.0
80	594.0
81	431.0
82	268.0
83	208.0
84	148.0
85	121.5
86	95.0
87	53.5
88	12.0
89	12.0
90	12.0
91	9.5
92	7.0
93	6.5
94	6.0
95	4.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1563133.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.74055652836495
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.28879555280473	12.637903249932592
2	6.60660381868435	2.0798324173702554
3	2.23725978360403	1.056471422773703
4	1.16434065855281	0.7330947981689672
5	0.7096903365994734	0.5585460430439181
6	0.4730769916884487	0.4467897077964517
7	0.3727744788855675	0.41073744300610426
8	0.2873110541470171	0.36179487112201963
9	0.256223476900761	0.3629790109845576
>10	3.9721681392293458	16.124875090929073
>50	1.9864999965216519	22.63390485886608
>100	1.6358448988068472	40.85632652366051
>500	0.006955818729293747	0.7261660958410008
>1k	0.0020458290380275727	0.6683304280746842
>5k	4.0916580760551454E-4	0.34224803843007345
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5314	0.3399582761031851	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3915	0.25045853423860925	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2505	0.16025507746301818	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.018296587686396488	0.0
2	0.0	0.0	0.0	0.0622467825834398	0.0
3	0.0	0.0	0.0	0.08790038979408663	0.0
4	0.0	0.0	0.0	0.14125477486560645	0.0
5	0.0	0.0	0.0	0.23644821010112382	0.0
6	0.0	0.0	0.0	0.3322813861648369	0.0
7	0.0	0.0	0.0	0.3881307604663199	0.0
8	0.0	0.0	0.0	0.5263787534394067	0.0
9	0.0	0.0	0.0	0.5762145639558502	0.0
10	6.397408281956813E-5	0.0	0.0	0.6660981503173434	0.0
11	6.397408281956813E-5	0.0	0.0	0.7747261429449701	0.0
12	6.397408281956813E-5	0.0	0.0	0.8691518891866526	0.0
13	6.397408281956813E-5	0.0	0.0	0.9026743085841064	0.0
14	1.2794816563913627E-4	0.0	0.0	0.916364762307494	0.0
15	1.2794816563913627E-4	0.0	0.0	0.9372203133066732	0.0
16	1.2794816563913627E-4	0.0	0.0	0.99102251695793	0.0
17	1.2794816563913627E-4	0.0	0.0	1.0523096882990763	0.0
18	1.2794816563913627E-4	0.0	0.0	1.1411696893354564	0.0
19	1.2794816563913627E-4	0.0	0.0	1.18358450624483	0.0
20	1.919222484587044E-4	0.0	0.0	1.2275347011418734	0.0
21	1.919222484587044E-4	0.0	0.0	1.286390857335876	0.0
22	1.919222484587044E-4	0.0	0.0	1.3476140545942028	0.0
23	1.919222484587044E-4	0.0	0.0	1.4232314204869323	0.0
24	1.919222484587044E-4	0.0	0.0	1.4859899957329288	0.0
25	1.919222484587044E-4	0.0	0.0	1.5347382468414397	0.0
26	1.919222484587044E-4	0.0	0.0	1.5848939277719811	0.0
27	1.919222484587044E-4	0.0	0.0	1.6311471896505287	0.0
28	1.919222484587044E-4	0.0	0.0	1.6834779893969356	0.0
29	1.919222484587044E-4	0.0	0.0	1.739519285946877	0.0
30	1.919222484587044E-4	0.0	0.0	1.8131534552722002	0.0
31	1.919222484587044E-4	0.0	0.0	1.882053542468875	0.0
32	1.919222484587044E-4	0.0	0.0	1.951977214990663	0.0
33	1.919222484587044E-4	0.0	0.0	2.017038857218164	0.0
34	1.919222484587044E-4	0.0	0.0	2.0860668925804777	0.0
35	1.919222484587044E-4	0.0	0.0	2.176718167935806	0.0
36	1.919222484587044E-4	0.0	0.0	2.25169579300034	0.0
37	1.919222484587044E-4	0.0	0.0	2.331343526110702	0.0
38	1.919222484587044E-4	0.0	0.0	2.406001280761138	0.0
39	1.919222484587044E-4	0.0	0.0	2.486736573279433	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGGC	20	7.034067E-4	45.0	3
CAAACCG	20	7.034067E-4	45.0	10
TCGATAG	45	3.8562575E-10	45.0	1
CCCGTAA	20	7.034067E-4	45.0	39
CCGTAAC	20	7.034067E-4	45.0	40
GGTATAC	25	3.891452E-5	45.0	8
CATACGT	40	6.8175723E-9	45.0	39
CATACGC	20	7.034067E-4	45.0	20
CGGTCTA	110	0.0	45.0	31
TATACGC	20	7.034067E-4	45.0	1
GCGTATT	20	7.034067E-4	45.0	31
TGACGTA	30	2.1660053E-6	44.999996	26
CTCACGA	115	0.0	43.04348	24
AGTACGG	165	0.0	42.272728	2
ATGTACG	65	0.0	41.53846	1
TCACGAC	120	0.0	41.249996	25
TAATGCG	55	6.184564E-11	40.909092	1
TCGAACG	55	6.184564E-11	40.909092	1
ATGGGCG	430	0.0	40.813953	5
GGCGATA	625	0.0	40.32	8
>>END_MODULE