FastQCFastQC Report
Thu 26 May 2016
SRR1545537_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545537_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1869685
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72160.38594736546530567No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC32350.17302379812642238No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG30420.16270120367869453No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG26010.13911434279036308No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGC351.2124656E-745.00000415
CTATACG207.0344214E-445.0000041
TTACGTC207.0344214E-445.00000426
CGTGTAA207.0344214E-445.00000441
TGCGTAA302.1662345E-645.0000041
ACGCATT253.8917475E-545.041
CCGTAAG253.8917475E-545.01
TATTACG253.8917475E-545.01
ACGTATG453.8562575E-1045.01
TACGTCC253.8917475E-545.035
TAATGCG650.044.9999961
GTTTACG950.042.631581
CTATGCG900.042.51
ATGACGT700.041.78571731
AATGCGG2050.041.7073172
TCGCTAA650.041.5384611
TATCGCG650.041.538461
TATTGCG1150.041.086961
TGGGCGA7050.040.5319146
CGCATGG1000.040.52
Produced by FastQC (version 0.11.5)