##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545537_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1869685
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.329245835528443	31.0	31.0	33.0	30.0	34.0
2	31.66756592688073	31.0	31.0	34.0	30.0	34.0
3	31.763679978178143	31.0	31.0	34.0	30.0	34.0
4	35.46319674169713	37.0	35.0	37.0	33.0	37.0
5	35.32344699775631	37.0	35.0	37.0	33.0	37.0
6	35.39837512736103	37.0	35.0	37.0	33.0	37.0
7	35.8430329173096	37.0	35.0	37.0	35.0	37.0
8	35.87252398131236	37.0	35.0	37.0	35.0	37.0
9	37.5375648839243	39.0	37.0	39.0	35.0	39.0
10	36.923343236962374	39.0	37.0	39.0	32.0	39.0
11	36.5263175347719	39.0	35.0	39.0	32.0	39.0
12	35.66955075320174	37.0	35.0	39.0	31.0	39.0
13	35.29278140435421	37.0	35.0	39.0	30.0	39.0
14	36.24740584643937	38.0	35.0	40.0	31.0	41.0
15	36.49543104854561	38.0	35.0	40.0	31.0	41.0
16	36.67372953197999	38.0	35.0	40.0	32.0	41.0
17	36.62735701468429	38.0	35.0	40.0	32.0	41.0
18	36.559626889021416	38.0	35.0	40.0	31.0	41.0
19	36.4820673001067	37.0	35.0	40.0	31.0	41.0
20	36.34814153186232	37.0	35.0	40.0	31.0	41.0
21	36.14369532835745	37.0	35.0	40.0	31.0	41.0
22	36.06439587417132	37.0	35.0	40.0	30.0	41.0
23	36.02864439731827	37.0	34.0	40.0	30.0	41.0
24	35.956486253031926	37.0	34.0	40.0	30.0	41.0
25	35.837422346545004	37.0	34.0	40.0	30.0	41.0
26	35.74803456197167	37.0	34.0	40.0	30.0	41.0
27	35.67414992365024	36.0	34.0	40.0	30.0	41.0
28	35.63966978394756	36.0	34.0	40.0	30.0	41.0
29	35.641232079200506	36.0	34.0	40.0	30.0	41.0
30	35.56611514773879	36.0	34.0	40.0	30.0	41.0
31	35.39877305535425	36.0	34.0	40.0	29.0	41.0
32	35.21804261145594	36.0	34.0	40.0	29.0	41.0
33	35.0463243808449	36.0	34.0	40.0	27.0	41.0
34	34.887831907513835	36.0	34.0	40.0	27.0	41.0
35	34.73726750762829	36.0	34.0	40.0	26.0	41.0
36	34.56025801137625	36.0	33.0	40.0	25.0	41.0
37	34.44948534111361	36.0	33.0	40.0	25.0	41.0
38	34.43926383321255	35.0	33.0	40.0	25.0	41.0
39	34.38397537553117	35.0	33.0	40.0	24.0	41.0
40	34.199322880592185	35.0	33.0	40.0	23.0	41.0
41	34.172749955206356	35.0	33.0	40.0	24.0	41.0
42	34.11351591310836	35.0	33.0	40.0	24.0	41.0
43	34.04780056533587	35.0	33.0	40.0	23.0	41.0
44	33.975323115925946	35.0	33.0	40.0	23.0	41.0
45	33.91597408119549	35.0	33.0	40.0	23.0	41.0
46	33.905189911669616	35.0	33.0	40.0	23.0	41.0
47	33.847973856558724	35.0	33.0	39.0	23.0	41.0
48	33.78282919315286	35.0	33.0	39.0	23.0	41.0
49	33.71295913482752	35.0	33.0	39.0	24.0	41.0
50	33.566502913592394	35.0	33.0	39.0	24.0	41.0
51	33.400690490644145	35.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	7.0
14	15.0
15	65.0
16	204.0
17	500.0
18	1159.0
19	2288.0
20	3709.0
21	5980.0
22	8765.0
23	12720.0
24	17684.0
25	24210.0
26	31788.0
27	37605.0
28	42034.0
29	47325.0
30	55601.0
31	67892.0
32	83601.0
33	108283.0
34	186905.0
35	277215.0
36	132042.0
37	159844.0
38	217772.0
39	344279.0
40	192.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.63856211072988	23.246643151119038	27.806341709967185	14.308453028183893
2	31.94040707391887	25.70416941891281	29.165982505074382	13.189441002093934
3	29.945686038022444	25.35475227110449	30.914833247311712	13.784728443561347
4	26.96475609527808	27.607484683248785	30.62494484365013	14.802814377823001
5	23.983184333189815	32.05047909139775	29.75297978001642	14.213356795396015
6	21.923586058614152	41.699002773194415	26.17366026897579	10.203750899215644
7	86.42776724421493	4.493323741699805	7.130559425785626	1.9483495882996333
8	87.33733222441214	3.6269211123798932	6.92186116912742	2.1138854940805536
9	83.15352586130818	5.291586550675649	8.588612520290852	2.966275067725312
10	52.46049468225931	21.482442229573433	14.197204341907863	11.859858746259397
11	44.96233322725486	21.721466450230924	18.95067885766854	14.365521464845681
12	40.14109328576739	20.334227423336017	24.71844187657279	14.806237414323803
13	22.986706316839467	36.8990498399463	24.68982743082391	15.424416412390324
14	16.853748091256012	40.058084650622966	27.55432064759572	15.533846610525304
15	16.14614226460607	23.091590294621824	45.79905171191939	14.963215728852722
16	17.924784121389433	17.92387487731891	44.63784006396799	19.513500937323666
17	17.50610396938522	18.22189299266989	29.41030173531905	34.86170130262584
18	21.584010140745633	23.17582908350872	34.103070838135835	21.13708993760981
19	29.387891543227866	22.70751490224289	26.788576685377485	21.116016869151753
20	31.688065101875452	21.89053236240329	25.41492283459513	21.00647970112613
21	24.137488400452483	25.73946948282732	28.87411515843578	21.248926958284418
22	23.968582943116086	22.967398251577137	25.500070867552555	27.563947937754218
23	21.40627966743061	27.910049018952392	25.06336628897381	25.620305024643187
24	20.04765508628459	24.16936542786619	36.97569376659705	18.807285719252175
25	20.128149928998734	23.24653618122839	34.717078010467006	21.908235879305874
26	19.19633521154633	31.789151648539725	26.655934020971443	22.358579118942494
27	20.44167867849397	32.49563429133784	27.023856959862226	20.03883007030596
28	17.30425178572861	29.227008827690227	34.85389250060839	18.61484688597277
29	18.840178960627057	24.551889756830697	34.465805737330086	22.14212554521216
30	19.345076844495196	29.20871697638907	30.84327039046684	20.602935788648892
31	28.633860784035814	26.452263349173794	25.18504453958822	19.728831327202176
32	30.14154790780265	25.045983681743184	26.77670302751533	18.035765382938838
33	28.48634930482942	26.93475104095075	24.759571799527727	19.8193278546921
34	21.75601772491088	26.93544634523997	27.460668508331615	23.84786742151753
35	21.63455341407777	25.576928733984598	30.163744160112532	22.62477369182509
36	31.42481220098573	25.105833335561872	24.409566317320834	19.059788146131567
37	22.277923821392374	32.33116808446342	27.220521103822303	18.170386990321898
38	22.323760419535912	31.64629335957661	23.39789857649818	22.632047644389296
39	23.1005222804911	30.177436306115734	25.96875944343566	20.753281969957506
40	24.983513265603563	25.240561912835584	26.49200266355028	23.283922158010572
41	18.479262549573857	25.06999842219411	27.502333280739805	28.948405747492224
42	22.64841403765875	26.679093002297176	24.59457074320006	26.077922216844012
43	23.22369810957461	25.653572660635348	25.63854339099902	25.484185838791024
44	21.049428112222113	29.000232659512164	27.12483653663585	22.825502691629872
45	18.76235836517916	34.64230605690263	24.040680649414206	22.554654928504
46	22.69376927129436	31.565477607190516	25.984751442087838	19.756001679427282
47	23.93702682537433	27.677389506788575	25.82317342226097	22.562410245576125
48	24.559752043793473	24.238949341734035	29.855082540641874	21.346216073830618
49	22.96263809144321	24.970035059381658	30.75095537483587	21.31637147433926
50	21.654877693301277	31.70972650473208	26.129642158973304	20.505753642993337
51	20.803343878781718	32.615066174248604	24.121122007183025	22.46046793978665
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1466.0
1	2908.0
2	4350.0
3	9504.0
4	14658.0
5	11009.0
6	7360.0
7	7561.5
8	7763.0
9	7887.0
10	8011.0
11	8213.0
12	8415.0
13	8153.0
14	7891.0
15	7840.0
16	7789.0
17	7260.5
18	6732.0
19	6717.5
20	6703.0
21	7147.5
22	7592.0
23	7100.0
24	6608.0
25	8631.5
26	11829.5
27	13004.0
28	16776.5
29	20549.0
30	24912.0
31	29275.0
32	29327.0
33	29379.0
34	35676.5
35	41974.0
36	42894.0
37	43814.0
38	55442.0
39	67070.0
40	87655.5
41	108241.0
42	138100.5
43	167960.0
44	171134.5
45	174309.0
46	171075.0
47	167841.0
48	159621.5
49	151402.0
50	142595.0
51	133788.0
52	122230.0
53	110672.0
54	101132.5
55	91593.0
56	88387.5
57	85182.0
58	79775.5
59	74369.0
60	69926.0
61	65483.0
62	58767.5
63	52052.0
64	45863.0
65	39674.0
66	34342.5
67	29011.0
68	25326.5
69	21642.0
70	19089.0
71	16536.0
72	14457.0
73	12378.0
74	10043.5
75	6217.5
76	4726.0
77	3259.5
78	1793.0
79	1416.5
80	1040.0
81	813.0
82	586.0
83	509.0
84	432.0
85	302.5
86	173.0
87	96.5
88	20.0
89	13.5
90	7.0
91	5.0
92	3.0
93	2.0
94	1.0
95	1.5
96	2.0
97	2.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1869685.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.581543662695378
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.7726973242624	12.585633101116725
2	7.04671657977543	2.1959744413282056
3	2.454219886338961	1.1472160295053742
4	1.2153737757438736	0.7574959821299243
5	0.7358682925858885	0.5732981965460058
6	0.4719549266869434	0.4412271778198082
7	0.3661696655640864	0.3993842042358964
8	0.2605857011448573	0.3248261984210146
9	0.23289052463127913	0.32659144903532783
>10	3.0656289784413624	12.108316125001144
>50	1.5548763061879431	17.680321619029584
>100	1.8064105484386934	48.92160978659629
>500	0.014531553923467706	1.5267449685456158
>1k	0.0017299468956509176	0.6223426524325772
>5k	3.4598937913018346E-4	0.3890180682565383
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7216	0.38594736546530567	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3235	0.17302379812642238	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	3042	0.16270120367869453	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2601	0.13911434279036308	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.348494532501464E-5	0.0	0.0	0.02583322859198207	0.0
2	5.348494532501464E-5	0.0	0.0	0.08300863514442272	0.0
3	5.348494532501464E-5	0.0	0.0	0.11574142168333168	0.0
4	5.348494532501464E-5	0.0	0.0	0.1892297365599018	0.0
5	1.0696989065002929E-4	0.0	0.0	0.3119776860808104	0.0
6	1.0696989065002929E-4	0.0	0.0	0.4371859430866697	0.0
7	1.0696989065002929E-4	0.0	0.0	0.5107812278538898	0.0
8	1.0696989065002929E-4	0.0	0.0	0.6973367171475409	0.0
9	2.1393978130005857E-4	0.0	0.0	0.7621604708814587	0.0
10	2.1393978130005857E-4	0.0	0.0	0.8844270558944421	0.0
11	2.1393978130005857E-4	0.0	0.0	1.0172836600817785	0.0
12	2.1393978130005857E-4	0.0	0.0	1.1321693226399099	0.0
13	2.1393978130005857E-4	0.0	0.0	1.1753316735171968	0.0
14	2.1393978130005857E-4	0.0	0.0	1.1897191238096256	0.0
15	2.1393978130005857E-4	0.0	0.0	1.2168359910894082	0.0
16	2.674247266250732E-4	0.0	0.0	1.282622473839176	0.0
17	2.674247266250732E-4	0.0	0.0	1.359480340271222	0.0
18	2.674247266250732E-4	0.0	0.0	1.476024036134429	0.0
19	2.674247266250732E-4	0.0	0.0	1.5276370083730682	0.0
20	2.674247266250732E-4	0.0	0.0	1.5833683214017333	0.0
21	2.674247266250732E-4	0.0	0.0	1.6578728502394788	0.0
22	3.2090967195008783E-4	0.0	0.0	1.7351051112888	0.0
23	3.2090967195008783E-4	0.0	0.0	1.8284363408809505	0.0
24	3.2090967195008783E-4	0.0	0.0	1.9011223815776455	0.0
25	3.2090967195008783E-4	0.0	0.0	1.9606511257243868	0.0
26	3.2090967195008783E-4	0.0	0.0	2.0215704784495783	0.0
27	3.2090967195008783E-4	0.0	0.0	2.080029523689819	0.0
28	3.2090967195008783E-4	0.0	0.0	2.143997518298537	0.0
29	3.2090967195008783E-4	0.0	0.0	2.2123512784239057	0.0
30	3.2090967195008783E-4	0.0	0.0	2.297285371600029	0.0
31	3.2090967195008783E-4	0.0	0.0	2.382059009940177	0.0
32	3.2090967195008783E-4	0.0	0.0	2.4564030839419475	0.0
33	3.743946172751025E-4	0.0	0.0	2.532244736412818	0.0
34	3.743946172751025E-4	0.0	0.0	2.615574281229191	0.0
35	3.743946172751025E-4	0.0	0.0	2.72040477406622	0.0
36	3.743946172751025E-4	0.0	0.0	2.8113826660640697	0.0
37	3.743946172751025E-4	0.0	0.0	2.9059440493986957	0.0
38	3.743946172751025E-4	0.0	0.0	2.9966545166699206	0.0
39	3.743946172751025E-4	0.0	0.0	3.089236957027521	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGC	35	1.2124656E-7	45.000004	15
CTATACG	20	7.0344214E-4	45.000004	1
TTACGTC	20	7.0344214E-4	45.000004	26
CGTGTAA	20	7.0344214E-4	45.000004	41
TGCGTAA	30	2.1662345E-6	45.000004	1
ACGCATT	25	3.8917475E-5	45.0	41
CCGTAAG	25	3.8917475E-5	45.0	1
TATTACG	25	3.8917475E-5	45.0	1
ACGTATG	45	3.8562575E-10	45.0	1
TACGTCC	25	3.8917475E-5	45.0	35
TAATGCG	65	0.0	44.999996	1
GTTTACG	95	0.0	42.63158	1
CTATGCG	90	0.0	42.5	1
ATGACGT	70	0.0	41.785717	31
AATGCGG	205	0.0	41.707317	2
TCGCTAA	65	0.0	41.53846	11
TATCGCG	65	0.0	41.53846	1
TATTGCG	115	0.0	41.08696	1
TGGGCGA	705	0.0	40.531914	6
CGCATGG	100	0.0	40.5	2
>>END_MODULE