FastQCFastQC Report
Thu 26 May 2016
SRR1545535_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545535_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1215663
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46250.3804508321796419No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC32250.26528733703337193No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTTC207.033448E-445.032
TTCTACG253.89094E-545.034
CAAACGA207.033448E-445.029
GTCGACG207.033448E-445.01
GACGTAG551.8189894E-1245.01
TAGAACG551.8189894E-1245.01
CTAAGCG253.89094E-545.01
TGACGTA253.89094E-545.039
CGTAGGA207.033448E-445.016
TCGCTAA207.033448E-445.015
TCGTAGC351.2120108E-745.044
ACCCGTG253.89094E-545.020
AATACGG1000.045.02
AGTACGT207.033448E-445.02
GTTTACG302.165607E-644.9999961
TCGTATA302.165607E-644.99999629
TTACGAG302.165607E-644.9999961
ACGTAAG600.044.9999961
ACGGGTA900.042.55
GTTACGG1450.041.896552
Produced by FastQC (version 0.11.5)