##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545535_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1215663
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.303195869249947	31.0	31.0	33.0	30.0	34.0
2	31.64546506721024	31.0	31.0	34.0	30.0	34.0
3	31.748902450761435	31.0	31.0	34.0	30.0	34.0
4	35.458021672124595	37.0	35.0	37.0	33.0	37.0
5	35.316442961577344	37.0	35.0	37.0	33.0	37.0
6	35.3967917095445	37.0	35.0	37.0	33.0	37.0
7	35.82901840394912	37.0	35.0	37.0	35.0	37.0
8	35.85275113250959	37.0	35.0	37.0	35.0	37.0
9	37.5221093345771	39.0	37.0	39.0	35.0	39.0
10	36.89423878163603	39.0	37.0	39.0	32.0	39.0
11	36.52519242586144	39.0	35.0	39.0	32.0	39.0
12	35.773824653707486	37.0	35.0	39.0	31.0	39.0
13	35.44773674941164	37.0	35.0	39.0	30.0	39.0
14	36.42712906455161	38.0	35.0	41.0	31.0	41.0
15	36.6384285776568	38.0	35.0	41.0	31.0	41.0
16	36.80114225735257	38.0	35.0	41.0	32.0	41.0
17	36.74386651563797	38.0	35.0	41.0	32.0	41.0
18	36.69023158556278	38.0	35.0	40.0	31.0	41.0
19	36.63579544659992	38.0	35.0	40.0	31.0	41.0
20	36.48979610303184	38.0	35.0	40.0	31.0	41.0
21	36.314625846143215	38.0	35.0	40.0	31.0	41.0
22	36.23335003204013	38.0	35.0	40.0	30.0	41.0
23	36.165199565998144	38.0	35.0	40.0	30.0	41.0
24	36.105015123434704	37.0	35.0	40.0	30.0	41.0
25	35.986799795667054	37.0	34.0	40.0	30.0	41.0
26	35.92008558292882	37.0	34.0	40.0	30.0	41.0
27	35.86324088172462	37.0	34.0	40.0	30.0	41.0
28	35.835773565535845	37.0	34.0	40.0	30.0	41.0
29	35.83471488397689	37.0	34.0	40.0	30.0	41.0
30	35.738731868947234	37.0	34.0	40.0	30.0	41.0
31	35.58090770221681	37.0	34.0	40.0	29.0	41.0
32	35.40148215418253	37.0	34.0	40.0	29.0	41.0
33	35.2391550947919	37.0	34.0	40.0	27.0	41.0
34	35.05902787203362	37.0	34.0	40.0	27.0	41.0
35	34.921907633941316	36.0	34.0	40.0	26.0	41.0
36	34.75506287515537	36.0	33.0	40.0	25.0	41.0
37	34.68742488666678	36.0	33.0	40.0	25.0	41.0
38	34.66571492263892	36.0	33.0	40.0	26.0	41.0
39	34.60691984538478	36.0	33.0	40.0	25.0	41.0
40	34.45892241517592	36.0	33.0	40.0	24.0	41.0
41	34.40835988263195	36.0	33.0	40.0	24.0	41.0
42	34.31205770020146	36.0	33.0	40.0	24.0	41.0
43	34.24318417192923	35.0	33.0	40.0	24.0	41.0
44	34.17735013733247	35.0	33.0	40.0	24.0	41.0
45	34.15134128455008	35.0	33.0	40.0	23.0	41.0
46	34.108825389931255	35.0	33.0	40.0	23.0	41.0
47	34.053691689226376	35.0	33.0	40.0	23.0	41.0
48	33.997796264260735	35.0	33.0	40.0	23.0	41.0
49	33.92875657151694	35.0	33.0	39.0	24.0	41.0
50	33.784979060808794	35.0	33.0	39.0	24.0	41.0
51	33.64486786222827	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	1.0
13	5.0
14	11.0
15	54.0
16	114.0
17	357.0
18	730.0
19	1346.0
20	2228.0
21	3708.0
22	5453.0
23	7867.0
24	11147.0
25	15382.0
26	19757.0
27	23628.0
28	26536.0
29	30304.0
30	35317.0
31	43082.0
32	53148.0
33	68297.0
34	113837.0
35	169033.0
36	87382.0
37	107215.0
38	147353.0
39	242234.0
40	135.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.20794825539644	22.917535534107724	27.383082318043733	14.491433892452102
2	32.20300362847269	25.181567589044	28.800744943294315	13.814683839188985
3	30.237245025965255	25.654725034816394	30.526552177700562	13.581477761517787
4	27.01168004619701	28.017057358823948	29.469844850094145	15.50141774488489
5	23.036318453387164	32.215342574381225	29.86633631195488	14.882002660276738
6	21.150680739645775	42.23793929732171	26.165557395429488	10.445822567603027
7	86.01462740907635	4.686742954256237	7.128291310996551	2.170338325670848
8	86.87753102627948	3.8849582491200274	7.3066302091944895	1.9308805154059965
9	82.72703866120791	5.257707111263565	8.751520775083225	3.263733452445291
10	48.74640422551316	23.89305259763602	15.111753833093546	12.248789343757274
11	42.20371928733539	22.340319644506742	21.011086131600617	14.444874936557254
12	38.4996499852344	21.349749066970038	25.73945246338829	14.411148484407274
13	23.11948294881065	35.94738015387488	25.109179106380637	15.823957790933838
14	17.017545158485532	38.25599693336064	28.245821415968074	16.480636492185745
15	16.17463063365423	24.20382951525217	43.96621432090966	15.655325530183942
16	18.289196923818526	19.47365347139791	42.65499566902999	19.582153935753578
17	18.10699182256925	19.497097468624116	30.18451659711614	32.211394111690495
18	21.594636013434645	23.239088464483988	33.60775149033902	21.558524031742348
19	29.04151890778941	23.87947975713664	26.382887362698384	20.696113972375567
20	30.075933873121087	22.580435531886714	26.680502738012095	20.6631278569801
21	24.03190686892667	26.236547464223225	28.52624452664924	21.20530114020086
22	23.246820870586667	24.452829443686287	25.714116494456114	26.586233191270935
23	20.71947570996238	28.759368344681054	26.470164840091375	24.050991105265194
24	20.075629512455343	24.16985628418402	37.568717646255585	18.185796557105053
25	19.331591074171047	25.143563635645734	34.52519324845784	20.99965204172538
26	18.603840044486013	31.737249550245423	27.837566825674546	21.821343579594014
27	18.814260202046125	32.41844162403561	28.338857068118383	20.42844110579988
28	16.794292497180553	28.689612170478167	35.4631999164242	19.052895415917074
29	17.971756975411772	24.824807533008737	34.7326520589999	22.470783432579587
30	19.75333624532457	30.38991891667345	29.676316544963534	20.18042829303845
31	27.4362220450898	26.778885266722767	25.827141238978236	19.957751449209198
32	28.398495306676274	25.614500071154588	27.620648156602613	18.36635646556653
33	27.64121306645016	27.759749206811428	25.38154077240156	19.217496954336852
34	19.86973363506169	27.941378490584974	28.38327727338909	23.805610600964247
35	21.522083011492494	25.093549774896495	31.409280367996722	21.975086845614285
36	29.486214518332794	26.07663472524869	25.922644680310253	18.514506076108265
37	21.812953096376216	31.895928394629102	27.820374561041998	18.47074394795268
38	20.88489984477606	31.81449135163281	25.620175986272507	21.680432817318614
39	21.642017565723396	31.107716529992274	26.555632605417784	20.694633298866545
40	23.796726559910105	26.193361153543375	26.628514645917495	23.38139764062902
41	18.630738946566606	25.651105610683224	27.6677006703338	28.050454772416366
42	21.799627034795005	26.472714888912467	25.07701558737907	26.650642488913455
43	21.58460033742904	26.20701625368215	27.030517503617368	25.177865905271446
44	20.21374344699148	28.77878161957714	28.82764384537491	22.179831088056474
45	18.47847635405536	35.288727221277604	24.159573829260246	22.073222595406786
46	21.222246625915243	32.672788429030085	26.437096465056513	19.66786847999816
47	22.427021304424006	28.34025548198802	26.586726749107275	22.6459964644807
48	22.063269179040574	25.60471117406716	30.25229853997366	22.079721106918612
49	21.75175192466991	25.403586355758133	31.798039423754776	21.04662229581718
50	20.16348280732407	31.607526098927085	27.37724188364703	20.851749210101815
51	19.511410645878012	31.674320926111925	25.89772000957502	22.916548418435042
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	691.0
1	1738.0
2	2785.0
3	6033.5
4	9282.0
5	7038.0
6	4794.0
7	4856.0
8	4918.0
9	5069.5
10	5221.0
11	5579.0
12	5937.0
13	5875.5
14	5814.0
15	5678.5
16	5543.0
17	5205.0
18	4867.0
19	4879.5
20	4892.0
21	4996.0
22	5100.0
23	6014.0
24	6928.0
25	8062.5
26	10402.5
27	11608.0
28	13675.5
29	15743.0
30	16878.0
31	18013.0
32	23262.5
33	28512.0
34	32853.5
35	37195.0
36	38853.5
37	40512.0
38	43732.5
39	46953.0
40	61972.5
41	76992.0
42	89339.5
43	101687.0
44	106944.5
45	112202.0
46	108335.0
47	104468.0
48	95850.0
49	87232.0
50	85608.0
51	83984.0
52	77958.5
53	71933.0
54	64035.5
55	56138.0
56	52610.0
57	49082.0
58	46931.0
59	44780.0
60	43922.5
61	43065.0
62	37795.5
63	32526.0
64	28568.5
65	24611.0
66	20584.0
67	16557.0
68	14195.0
69	11833.0
70	10534.5
71	9236.0
72	7843.0
73	6450.0
74	5251.5
75	3072.5
76	2092.0
77	1691.0
78	1290.0
79	915.0
80	540.0
81	390.5
82	241.0
83	164.0
84	87.0
85	69.0
86	51.0
87	31.5
88	12.0
89	9.5
90	7.0
91	6.5
92	6.0
93	4.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1215663.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.66739310459396
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.64529613932037	14.071247558251725
2	6.32939363818651	2.2364777103911444
3	2.2220488384616965	1.1777343098012758
4	1.168484807678317	0.8257632173599477
5	0.7525078112524143	0.6647425657836998
6	0.53248618673801	0.5644585670319989
7	0.43248755869452665	0.5348649398611649
8	0.34906390548777094	0.4933639389501826
9	0.2965261325266486	0.47149593742199036
>10	5.049468564091517	22.631639299122295
>50	2.047491986924968	25.65083849493493
>100	1.1691258841375958	29.338287671026382
>500	0.003277485458135031	0.4016099277710101
>1k	0.002341061041525022	0.9374758622922468
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4625	0.3804508321796419	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3225	0.26528733703337193	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.019906832732426667	0.0
2	0.0	0.0	0.0	0.07288204049971085	0.0
3	0.0	0.0	0.0	0.10743108904359185	0.0
4	0.0	0.0	0.0	0.16624673120757974	0.0
5	0.0	0.0	0.0	0.2733487816936108	0.0
6	0.0	0.0	0.0	0.3760088116525715	0.0
7	0.0	0.0	0.0	0.4373745026376553	0.0
8	0.0	0.0	0.0	0.5811643522917124	0.0
9	0.0	0.0	0.0	0.6320830690742418	0.0
10	0.0	0.0	0.0	0.7297252610304007	0.0
11	0.0	0.0	0.0	0.8580502984791015	0.0
12	0.0	0.0	0.0	0.9665507628347659	0.0
13	0.0	0.0	0.0	1.008832217481325	0.0
14	0.0	0.0	0.0	1.0264357803108262	0.0
15	0.0	0.0	0.0	1.05366372094898	0.0
16	0.0	0.0	0.0	1.1170036432794286	0.0
17	0.0	0.0	0.0	1.1898856837791394	0.0
18	0.0	0.0	0.0	1.2922166751805393	0.0
19	0.0	0.0	0.0	1.3391046696329492	0.0
20	0.0	0.0	0.0	1.3867330008398708	0.0
21	0.0	0.0	0.0	1.4573940310760465	0.0
22	0.0	0.0	0.0	1.5347180921028278	0.0
23	0.0	0.0	0.0	1.6187051839202147	0.0
24	0.0	0.0	0.0	1.6878032810079766	0.0
25	0.0	0.0	0.0	1.7473592599264762	0.0
26	0.0	0.0	0.0	1.8014861026452231	0.0
27	0.0	0.0	0.0	1.855941983921531	0.0
28	0.0	0.0	0.0	1.9142640682491776	0.0
29	8.225963939019284E-5	0.0	0.0	1.9760410574312124	0.0
30	8.225963939019284E-5	0.0	0.0	2.055174830524578	0.0
31	8.225963939019284E-5	0.0	0.0	2.1380925470298924	0.0
32	8.225963939019284E-5	0.0	0.0	2.214347232744601	0.0
33	8.225963939019284E-5	0.0	0.0	2.2878873503594335	0.0
34	8.225963939019284E-5	0.0	0.0	2.3655404499437753	0.0
35	1.6451927878038567E-4	0.0	0.0	2.4682827395421265	0.0
36	2.4677891817057854E-4	0.0	0.0	2.5541618030654876	0.0
37	2.4677891817057854E-4	0.0	0.0	2.6453877431492114	0.0
38	2.4677891817057854E-4	0.0	0.0	2.7328297398209864	0.0
39	2.4677891817057854E-4	0.0	0.0	2.8210943328866636	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTTC	20	7.033448E-4	45.0	32
TTCTACG	25	3.89094E-5	45.0	34
CAAACGA	20	7.033448E-4	45.0	29
GTCGACG	20	7.033448E-4	45.0	1
GACGTAG	55	1.8189894E-12	45.0	1
TAGAACG	55	1.8189894E-12	45.0	1
CTAAGCG	25	3.89094E-5	45.0	1
TGACGTA	25	3.89094E-5	45.0	39
CGTAGGA	20	7.033448E-4	45.0	16
TCGCTAA	20	7.033448E-4	45.0	15
TCGTAGC	35	1.2120108E-7	45.0	44
ACCCGTG	25	3.89094E-5	45.0	20
AATACGG	100	0.0	45.0	2
AGTACGT	20	7.033448E-4	45.0	2
GTTTACG	30	2.165607E-6	44.999996	1
TCGTATA	30	2.165607E-6	44.999996	29
TTACGAG	30	2.165607E-6	44.999996	1
ACGTAAG	60	0.0	44.999996	1
ACGGGTA	90	0.0	42.5	5
GTTACGG	145	0.0	41.89655	2
>>END_MODULE