FastQCFastQC Report
Thu 26 May 2016
SRR1545534_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545534_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1975057
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC119240.6037294113536976No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85740.4341140534171925No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG650.045.00000425
CGTATTA502.1827873E-1145.031
ACGCACG207.034521E-445.014
CTATACG253.8918282E-545.017
ATTAACG502.1827873E-1145.01
GTTAGCG700.045.01
CGTTCCA253.8918282E-545.041
ATCGTTC351.2125201E-745.016
ATACTCC253.8918282E-545.037
TGCATCG207.034521E-445.024
TAGCACG253.8918282E-545.01
CCGTATT502.1827873E-1145.030
CGGATTA207.034521E-445.019
CGGTGAT207.034521E-445.043
TTACGTC207.034521E-445.032
CGTAAGA207.034521E-445.02
CCGAACG207.034521E-445.026
GTAAGAC253.8918282E-545.029
TCGACAT351.2125201E-745.034
TCGACAC207.034521E-445.023
Produced by FastQC (version 0.11.5)