##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545534_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1975057
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.332863811019124	31.0	31.0	33.0	30.0	34.0
2	31.656620036788812	31.0	31.0	34.0	30.0	34.0
3	31.71713221441204	31.0	31.0	34.0	30.0	34.0
4	35.40741608976349	37.0	35.0	37.0	33.0	37.0
5	35.275738371095116	37.0	35.0	37.0	33.0	37.0
6	35.358683319013075	37.0	35.0	37.0	33.0	37.0
7	35.87540815277736	37.0	35.0	37.0	35.0	37.0
8	35.91893702308339	37.0	35.0	37.0	35.0	37.0
9	37.614725549693	39.0	37.0	39.0	35.0	39.0
10	36.95073053587821	39.0	37.0	39.0	32.0	39.0
11	36.51181813993217	39.0	35.0	39.0	32.0	39.0
12	35.394057487961106	37.0	35.0	39.0	31.0	39.0
13	34.84897600423684	37.0	34.0	39.0	29.0	39.0
14	35.7407426722368	37.0	34.0	40.0	29.0	41.0
15	36.113290401239055	37.0	35.0	40.0	31.0	41.0
16	36.37568637259583	37.0	35.0	40.0	32.0	41.0
17	36.31553266563952	37.0	35.0	40.0	31.0	41.0
18	36.27260428433205	37.0	35.0	40.0	31.0	41.0
19	36.219317214642416	36.0	35.0	40.0	31.0	41.0
20	36.02367526608093	36.0	35.0	40.0	31.0	41.0
21	35.78794029741926	36.0	34.0	40.0	30.0	41.0
22	35.72017769613738	35.0	34.0	40.0	30.0	41.0
23	35.71465684281517	35.0	34.0	40.0	30.0	41.0
24	35.64997465895921	35.0	34.0	40.0	30.0	41.0
25	35.560172187435604	35.0	34.0	40.0	30.0	41.0
26	35.463171442646974	35.0	34.0	40.0	30.0	41.0
27	35.37531980089688	35.0	34.0	40.0	30.0	41.0
28	35.41758491020766	36.0	34.0	40.0	30.0	41.0
29	35.464283815606336	36.0	34.0	40.0	30.0	41.0
30	35.3759876297241	36.0	34.0	40.0	30.0	41.0
31	35.155541333743784	35.0	34.0	40.0	29.0	41.0
32	34.92777828690514	35.0	34.0	40.0	29.0	41.0
33	34.72939818952061	35.0	34.0	40.0	27.0	41.0
34	34.580111358811415	35.0	34.0	40.0	27.0	41.0
35	34.42618415569778	35.0	33.0	40.0	26.0	41.0
36	34.18829532514758	35.0	33.0	40.0	24.0	41.0
37	34.10997302862651	35.0	33.0	40.0	24.0	41.0
38	34.135285715804656	35.0	33.0	40.0	24.0	41.0
39	34.11340077780034	35.0	33.0	40.0	24.0	41.0
40	33.92683907350522	35.0	33.0	40.0	23.0	41.0
41	33.95133507539276	35.0	33.0	40.0	23.0	41.0
42	33.87969005451488	35.0	33.0	39.0	23.0	41.0
43	33.755897677889806	35.0	33.0	39.0	23.0	41.0
44	33.72932629286142	35.0	33.0	39.0	23.0	41.0
45	33.63762767353043	35.0	33.0	39.0	23.0	41.0
46	33.62963600544187	35.0	33.0	39.0	23.0	41.0
47	33.5749773297682	35.0	33.0	39.0	23.0	41.0
48	33.57643905973347	35.0	33.0	39.0	23.0	41.0
49	33.54631942267995	35.0	33.0	39.0	24.0	41.0
50	33.393360799207315	35.0	33.0	39.0	23.0	41.0
51	33.23888880169028	35.0	32.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	0.0
13	9.0
14	22.0
15	62.0
16	191.0
17	512.0
18	1144.0
19	2304.0
20	3998.0
21	6309.0
22	9524.0
23	14163.0
24	19668.0
25	27636.0
26	35661.0
27	41386.0
28	45388.0
29	51804.0
30	60539.0
31	74337.0
32	93058.0
33	121168.0
34	213061.0
35	350423.0
36	126867.0
37	144823.0
38	200639.0
39	330144.0
40	214.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.3365857289182	23.971915747241727	29.846581642960178	13.844916880879893
2	30.89227298250126	26.55634748769276	29.712712088815664	12.838667440990312
3	28.709399273033636	24.05728037216141	32.93560641540979	14.297713939395168
4	25.291472600537606	28.425255574902398	30.755669330049717	15.527602494510283
5	22.209485599656112	32.63419739278411	30.242063899927953	14.914253107631831
6	20.841778237286317	41.73818780926323	26.434781375929912	10.985252577520548
7	86.69795352741718	4.626296861305775	6.95934345185987	1.7164061594171713
8	88.0671798332909	3.630173711442252	6.564975086794964	1.7376713684718972
9	84.26222635599883	5.239038670782667	7.993389557870987	2.5053454153475063
10	54.711281750349485	22.726483336936603	12.816895917434282	9.74533899527963
11	49.57426545157937	20.383057299105797	18.71571301486489	11.326964234449942
12	45.76369188332286	20.284325971351713	22.088628328195085	11.863353817130342
13	20.558444642357156	45.21565706711249	22.174600530516333	12.051297760014016
14	14.002178164984604	47.475693106578696	26.12299290602752	12.399135822409175
15	12.775985705729	22.89463038281933	51.92923546003989	12.400148451411782
16	13.931901712203748	18.106566038347246	50.38831790677434	17.573214342674667
17	14.252196265728026	18.285193794406947	28.816788578760004	38.64582136110502
18	19.541917018091123	23.263126076867653	36.95837639116238	20.23658051387884
19	29.887188065964676	24.24760399320121	25.74644681140848	20.118761129425632
20	32.56290831100064	22.15525931656656	25.733485160175125	19.54834721225767
21	20.542850155717023	29.379253358257508	28.95708832707107	21.1208081589544
22	21.308954627638595	25.373748706999343	24.548709227126103	28.76858743823596
23	17.531038344716126	32.951504690750696	24.162897577133215	25.35455938739996
24	18.028694868046845	23.544890096842774	42.444901590181956	15.981513444928424
25	14.997592474546304	25.897379164246903	38.79493098173876	20.310097379468036
26	15.76627915042452	36.74921787067411	27.394500513149744	20.09000246575162
27	16.038828246475926	38.34486802153052	27.184734415259914	18.431569316733643
28	12.49265211079984	31.172163638821566	39.681285147719784	16.65389910265881
29	14.04688573544966	24.15666990876719	39.6876647104362	22.108779645346942
30	16.980471955999242	33.5301715342899	32.04267016091181	17.446686348799048
31	29.871694842224805	28.425306206352523	23.817135404193397	17.88586354722927
32	30.896931075913255	26.422629827898636	26.33103753461293	16.349401561575185
33	29.358899515305126	28.82357319307747	23.925993021973543	17.891534269643863
34	19.132257955086864	28.363485205743427	27.660619415034603	24.8436374241351
35	19.831832701537223	25.83733026439237	31.889256867017004	22.441580167053406
36	33.02851512639889	24.569063070078485	26.098132864013547	16.30428893950909
37	19.714671525935707	35.34029650789826	28.64985668768041	16.295175278485633
38	19.749759120876003	34.89230943714536	23.44291835628035	21.915013085698288
39	19.863224200618006	33.10031052268365	28.432343977920638	18.604121298777706
40	23.926347442124456	26.41736415708509	26.141270859524564	23.515017541265898
41	16.17295095787109	25.21203185528316	27.765780936955238	30.84923624989051
42	21.269917779588134	25.52245327603203	24.830321352750833	28.377307591629002
43	21.271234197291523	25.8179890504426	27.316933131550126	25.593843620715756
44	18.80381173809161	30.996421875419294	29.75007809901183	20.449688287477272
45	16.061106084533254	40.83254306078255	21.946607110579595	21.1597437441046
46	21.210577720035424	36.01065690762342	25.226411187120167	17.552354185220985
47	21.20374247426783	29.40958159688556	26.243191968636854	23.143483960209757
48	22.93493301712305	24.510279956477206	31.670174582303197	20.88461244409655
49	21.210729614385812	23.796629666890627	34.18584881347728	20.80679190524628
50	18.9236057490999	36.4878583251015	25.656424093076808	18.93211183272179
51	18.123122522539855	35.85678793067744	23.472892174757487	22.547197372025213
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2383.0
1	3070.0
2	3757.0
3	9000.5
4	14244.0
5	10633.0
6	7022.0
7	7760.0
8	8498.0
9	9508.0
10	10518.0
11	11470.5
12	12423.0
13	12545.5
14	12668.0
15	12153.0
16	11638.0
17	10888.5
18	10139.0
19	10116.5
20	10094.0
21	9537.0
22	8980.0
23	9359.0
24	9738.0
25	11214.5
26	13094.0
27	13497.0
28	17512.0
29	21527.0
30	27554.0
31	33581.0
32	36194.0
33	38807.0
34	46967.0
35	55127.0
36	56304.5
37	57482.0
38	69788.5
39	82095.0
40	113860.5
41	145626.0
42	177550.5
43	209475.0
44	222215.5
45	234956.0
46	221510.0
47	208064.0
48	188916.0
49	169768.0
50	156127.0
51	142486.0
52	124440.5
53	106395.0
54	93795.0
55	81195.0
56	71043.5
57	60892.0
58	56554.5
59	52217.0
60	44367.0
61	36517.0
62	33597.0
63	30677.0
64	24552.5
65	18428.0
66	14969.5
67	11511.0
68	10258.5
69	9006.0
70	8104.5
71	7203.0
72	6131.0
73	5059.0
74	4366.0
75	2879.0
76	2085.0
77	1590.5
78	1096.0
79	1022.0
80	948.0
81	790.0
82	632.0
83	379.5
84	127.0
85	86.0
86	45.0
87	36.0
88	27.0
89	22.5
90	18.0
91	11.0
92	4.0
93	4.0
94	4.0
95	8.5
96	13.0
97	7.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1975057.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.22026249804573
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.66091525977012	13.717818716078181
2	8.547851871939143	2.9439250605840726
3	2.9963193431333823	1.5479221685018638
4	1.5974339078803734	1.1003292486791614
5	0.9550418394408285	0.8223035585893755
6	0.6101814446484504	0.6304490788969846
7	0.45332574224390887	0.5464471794993071
8	0.3220042823489357	0.44359986140348046
9	0.2688222543955914	0.41662708074076604
>10	2.3203884048939627	8.964114499996159
>50	0.7748697823613343	9.721743374672913
>100	1.4679552252562416	55.10994538798498
>500	0.022520104384214975	2.555513844988808
>1k	0.001777902977701182	0.4333178301913853
>5k	2.96317162950197E-4	0.43750201084090173
>10k+	2.96317162950197E-4	0.6084410983516343
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	11924	0.6037294113536976	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8574	0.4341140534171925	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05478322904098464	0.0
2	0.0	0.0	0.0	0.18267827207012252	0.0
3	0.0	0.0	0.0	0.24566379603221578	0.0
4	0.0	0.0	0.0	0.36550843849063597	0.0
5	0.0	0.0	0.0	0.6065647725609944	0.0
6	5.0631450130300035E-5	0.0	0.0	0.8503552049383891	0.0
7	1.0126290026060007E-4	0.0	0.0	0.9948573636102654	0.0
8	1.0126290026060007E-4	0.0	0.0	1.3437080550080327	0.0
9	1.0126290026060007E-4	0.0	0.0	1.489729157183818	0.0
10	1.0126290026060007E-4	0.0	0.0	1.78708766379907	0.0
11	1.0126290026060007E-4	0.0	0.0	2.197506198555282	0.0
12	1.518943503909001E-4	0.0	0.0	2.497041857526137	0.0
13	1.518943503909001E-4	0.0	0.0	2.611418303370485	0.0
14	1.518943503909001E-4	0.0	0.0	2.6506070457713373	0.0
15	1.518943503909001E-4	0.0	0.0	2.713845726984082	0.0
16	1.518943503909001E-4	0.0	0.0	2.8705500651373606	0.0
17	1.518943503909001E-4	0.0	0.0	3.0556586468137374	0.0
18	1.518943503909001E-4	0.0	0.0	3.304259066953511	0.0
19	1.518943503909001E-4	0.0	0.0	3.4275972794709215	0.0
20	1.518943503909001E-4	0.0	0.0	3.5459229784254327	0.0
21	1.518943503909001E-4	0.0	0.0	3.6977666973662027	0.0
22	1.518943503909001E-4	0.0	0.0	3.859837969233293	0.0
23	1.518943503909001E-4	0.0	0.0	4.054870315135209	0.0
24	1.518943503909001E-4	0.0	0.0	4.1990180536561725	0.0
25	1.518943503909001E-4	0.0	0.0	4.310356612492702	0.0
26	1.518943503909001E-4	0.0	0.0	4.422404011631056	0.0
27	1.518943503909001E-4	0.0	0.0	4.531767943912505	0.0
28	1.518943503909001E-4	0.0	0.0	4.653739107276397	0.0
29	1.518943503909001E-4	0.0	0.0	4.784317617162442	0.0
30	1.518943503909001E-4	0.0	0.0	4.959299908812758	0.0
31	1.518943503909001E-4	0.0	0.0	5.11144741645431	0.0
32	1.518943503909001E-4	0.0	0.0	5.240557614286575	0.0
33	1.518943503909001E-4	0.0	0.0	5.378275158640991	0.0
34	2.0252580052120014E-4	0.0	0.0	5.521663425410001	0.0
35	2.0252580052120014E-4	0.0	0.0	5.704898643431557	0.0
36	2.0252580052120014E-4	0.0	0.0	5.864894025843305	0.0
37	2.0252580052120014E-4	0.0	0.0	6.024534988104141	0.0
38	2.0252580052120014E-4	0.0	0.0	6.1836190044135435	0.0
39	2.0252580052120014E-4	0.0	0.0	6.34158912882008	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCTAG	65	0.0	45.000004	25
CGTATTA	50	2.1827873E-11	45.0	31
ACGCACG	20	7.034521E-4	45.0	14
CTATACG	25	3.8918282E-5	45.0	17
ATTAACG	50	2.1827873E-11	45.0	1
GTTAGCG	70	0.0	45.0	1
CGTTCCA	25	3.8918282E-5	45.0	41
ATCGTTC	35	1.2125201E-7	45.0	16
ATACTCC	25	3.8918282E-5	45.0	37
TGCATCG	20	7.034521E-4	45.0	24
TAGCACG	25	3.8918282E-5	45.0	1
CCGTATT	50	2.1827873E-11	45.0	30
CGGATTA	20	7.034521E-4	45.0	19
CGGTGAT	20	7.034521E-4	45.0	43
TTACGTC	20	7.034521E-4	45.0	32
CGTAAGA	20	7.034521E-4	45.0	2
CCGAACG	20	7.034521E-4	45.0	26
GTAAGAC	25	3.8918282E-5	45.0	29
TCGACAT	35	1.2125201E-7	45.0	34
TCGACAC	20	7.034521E-4	45.0	23
>>END_MODULE