FastQCFastQC Report
Thu 26 May 2016
SRR1545533_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545533_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1396664
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC62610.448282478820962No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48870.34990520268296454No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGC453.8562575E-1045.00000423
GCTATCG302.165838E-645.00000438
GAACGAT302.165838E-645.00000443
CGTCGCA302.165838E-645.00000445
CGACTGG453.8562575E-1045.0000042
CCGTTAT302.165838E-645.00000439
TACACGG302.165838E-645.0000042
GACTAAC302.165838E-645.0000049
TCGCAAA207.033809E-445.030
CGTTGTA207.033809E-445.025
GATCCGA207.033809E-445.044
CGTTATT207.033809E-445.018
TGCGCAA207.033809E-445.037
ACGTGTA207.033809E-445.031
TCGCTAA207.033809E-445.026
TATTACG207.033809E-445.01
TTGCGAA207.033809E-445.028
CTCGCTA207.033809E-445.025
TCGTACT207.033809E-445.018
TATAGCG800.045.01