##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545533_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1396664
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.300022768539893	31.0	31.0	33.0	30.0	34.0
2	31.63882938201314	31.0	31.0	34.0	30.0	34.0
3	31.725098520474504	31.0	31.0	34.0	30.0	34.0
4	35.43354736715488	37.0	35.0	37.0	33.0	37.0
5	35.28027857809752	37.0	35.0	37.0	33.0	37.0
6	35.364567283183355	37.0	35.0	37.0	33.0	37.0
7	35.853533133237484	37.0	35.0	37.0	35.0	37.0
8	35.885083312808234	37.0	35.0	37.0	35.0	37.0
9	37.58912809380065	39.0	37.0	39.0	35.0	39.0
10	36.907040633967796	39.0	37.0	39.0	32.0	39.0
11	36.51361601645063	39.0	35.0	39.0	32.0	39.0
12	35.512179736858684	37.0	35.0	39.0	31.0	39.0
13	35.047585532382875	37.0	34.0	39.0	30.0	39.0
14	35.96205529748028	38.0	35.0	40.0	30.0	41.0
15	36.30864975398521	38.0	35.0	40.0	31.0	41.0
16	36.53432822783432	38.0	35.0	40.0	32.0	41.0
17	36.50753151795994	38.0	35.0	40.0	32.0	41.0
18	36.42316476976567	37.0	35.0	40.0	31.0	41.0
19	36.38516994781852	37.0	35.0	40.0	31.0	41.0
20	36.19666218933115	37.0	35.0	40.0	31.0	41.0
21	35.98883983549372	36.0	34.0	40.0	30.0	41.0
22	35.919980754139864	36.0	34.0	40.0	30.0	41.0
23	35.929827789647334	36.0	34.0	40.0	30.0	41.0
24	35.86803912752101	36.0	34.0	40.0	30.0	41.0
25	35.76205300630646	36.0	34.0	40.0	30.0	41.0
26	35.656430608936724	36.0	34.0	40.0	30.0	41.0
27	35.588129285211046	36.0	34.0	40.0	30.0	41.0
28	35.60519137029379	36.0	34.0	40.0	30.0	41.0
29	35.64440839027855	36.0	34.0	40.0	30.0	41.0
30	35.57659036103171	36.0	34.0	40.0	30.0	41.0
31	35.3778940389385	36.0	34.0	40.0	29.0	41.0
32	35.1820609681355	36.0	34.0	40.0	29.0	41.0
33	34.98339829765785	36.0	34.0	40.0	27.0	41.0
34	34.83015886426514	35.0	34.0	40.0	27.0	41.0
35	34.67171417033732	35.0	34.0	40.0	26.0	41.0
36	34.47839423082431	35.0	33.0	40.0	25.0	41.0
37	34.427960482979444	35.0	33.0	40.0	25.0	41.0
38	34.45334955293471	35.0	33.0	40.0	25.0	41.0
39	34.45287270238225	35.0	33.0	40.0	25.0	41.0
40	34.254268027242055	35.0	33.0	40.0	24.0	41.0
41	34.261101453177	35.0	33.0	40.0	24.0	41.0
42	34.19131731039105	35.0	33.0	40.0	24.0	41.0
43	34.08842856979202	35.0	33.0	40.0	24.0	41.0
44	34.045544955694425	35.0	33.0	40.0	23.0	41.0
45	34.020413642794544	35.0	33.0	40.0	23.0	41.0
46	33.999174461430954	35.0	33.0	40.0	23.0	41.0
47	33.93871754409078	35.0	33.0	40.0	23.0	41.0
48	33.95692879604543	35.0	33.0	40.0	23.0	41.0
49	33.914819885097636	35.0	33.0	39.0	24.0	41.0
50	33.78389505278292	35.0	33.0	39.0	24.0	41.0
51	33.593005189508716	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	3.0
13	7.0
14	9.0
15	54.0
16	141.0
17	346.0
18	824.0
19	1565.0
20	2690.0
21	4275.0
22	6446.0
23	9420.0
24	13292.0
25	18834.0
26	24582.0
27	28286.0
28	31064.0
29	34899.0
30	41357.0
31	50450.0
32	62259.0
33	81474.0
34	142043.0
35	227134.0
36	90200.0
37	106610.0
38	152412.0
39	265852.0
40	134.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.43218984666319	22.129731989941746	29.10084315196783	14.337235011427229
2	30.910655676669553	25.86305654044208	29.622801189119212	13.603486593769155
3	28.527906497196177	25.280167599365345	32.114595922856175	14.0773299805823
4	25.655490511676394	27.394777842057934	31.474427636138685	15.475304010126989
5	21.641998361810717	32.62925084343836	30.570058367653207	15.158692427097714
6	20.336315677929694	41.226236231477294	28.319624476609977	10.117823613983033
7	86.41856595430254	4.610557728988504	7.000037231574667	1.9708390851342916
8	87.23730260105509	3.780007217197551	6.919774548495558	2.062915633251806
9	84.00610311427802	4.972921189348333	8.531615334826414	2.48936036154723
10	52.31745072544292	21.960041928481008	15.409146365911916	10.313360980164163
11	47.50892125808355	20.91669864763465	19.27578859339111	12.298591500890693
12	43.09139492390439	21.57132996912643	23.79512896444671	11.542146142522467
13	20.66502752272558	44.05733948895368	21.81462398973554	13.463008998585199
14	14.414920123952504	44.48514460170807	27.293393400273796	13.806541874065632
15	12.691527811986274	24.03777859241736	50.36522742764187	12.905466167954497
16	13.851434561211573	18.414951627592608	48.214602796377655	19.519011014818165
17	14.61403744923618	19.211134531999107	29.46771736079687	36.707110657967846
18	20.124024103148646	23.213242411918685	37.29386595487533	19.368867530057337
19	29.026594800181005	25.690072916606997	25.691003705973664	19.592328577238334
20	32.09046699850501	22.241856309033526	27.066638790718457	18.601037901743013
21	20.951853846021663	28.64969670586483	29.263301696041427	21.13514775207208
22	21.711163171671927	25.195250969452925	25.34718443376503	27.74640142511012
23	17.886549664056638	31.808151423678137	25.19868773019137	25.106611182073856
24	18.80867552969075	23.241452489646758	42.10239542223469	15.847476558427797
25	15.51418236598065	27.024824868400703	36.360069422566916	21.10092334305173
26	16.94566481272518	35.33706030942302	27.614587330954333	20.102687546897464
27	15.955233327414467	37.33310230663925	28.531844452208976	18.179819913737305
28	13.506756098818325	29.931178866212633	39.35821357176816	17.203851463200884
29	14.293702708740256	24.42677694850014	38.22565771008632	23.053862632673283
30	17.06165548764771	32.88672150209356	31.93180321108012	18.119819799178615
31	27.720267723661525	28.817023994317886	25.89627856091372	17.566429721106864
32	29.719674882434145	25.714703035232528	27.293107003545593	17.27251507878774
33	27.690482463928333	29.439793679797	24.82851995898799	18.041203897286678
34	19.007076863153916	27.742463470097317	27.109383502402867	26.1410761643459
35	19.953403252321245	26.77981246742237	31.413138736303075	21.853645543953306
36	30.0765968049581	26.83966938361696	26.162054724686822	16.921679086738113
37	19.05290033966652	34.27051889359216	29.88156063305133	16.79502013368999
38	18.40406855192086	34.33746412880979	25.996445816602993	21.262021502666354
39	19.98476369405956	33.7242887337255	27.932129703350267	18.358817868864666
40	24.284294576218763	25.875013603844586	27.367713351242674	22.472978468693974
41	16.244207626172077	25.63100359141497	28.86793101275611	29.25685776965684
42	21.16464661507707	25.59191043801516	25.011097873217896	28.232345073689878
43	20.87824988687329	26.471219992782803	27.256877817427817	25.39365230291609
44	17.82669274786205	31.158102449837617	29.653374039854967	21.36183076244537
45	15.684946415172153	40.99017372825533	22.566272202906354	20.758607653666164
46	21.00240286854963	35.78512799069784	25.461313529954232	17.751155610798303
47	20.044835407800303	29.856787316061702	27.265469719273927	22.832907556864072
48	22.011378542011535	24.721980376096184	32.0062663604131	21.26037472147918
49	20.913190287714155	25.37969046241616	33.04875045107485	20.658368798794843
50	19.013807186266703	36.22818372922908	25.89269860181117	18.865310482693047
51	17.193254784257345	35.146033691711104	24.87949857660826	22.78121294742329
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1312.0
1	1670.0
2	2028.0
3	5550.0
4	9072.0
5	6946.0
6	4820.0
7	5268.0
8	5716.0
9	6108.5
10	6501.0
11	6923.5
12	7346.0
13	7413.5
14	7481.0
15	7111.5
16	6742.0
17	6273.0
18	5804.0
19	5890.0
20	5976.0
21	5995.5
22	6015.0
23	6667.5
24	7320.0
25	8199.5
26	12054.0
27	15029.0
28	16360.0
29	17691.0
30	21537.5
31	25384.0
32	30820.5
33	36257.0
34	40141.0
35	44025.0
36	46590.5
37	49156.0
38	58874.0
39	68592.0
40	92228.5
41	115865.0
42	135187.0
43	154509.0
44	152241.0
45	149973.0
46	142818.0
47	135663.0
48	123209.5
49	110756.0
50	100883.5
51	91011.0
52	82870.5
53	74730.0
54	64203.5
55	53677.0
56	47748.0
57	41819.0
58	38570.5
59	35322.0
60	32211.0
61	29100.0
62	24945.0
63	20790.0
64	17331.0
65	13872.0
66	11484.0
67	9096.0
68	7974.5
69	6853.0
70	6224.5
71	5596.0
72	4384.0
73	3172.0
74	2441.0
75	1370.5
76	1031.0
77	626.5
78	222.0
79	230.0
80	238.0
81	231.0
82	224.0
83	151.0
84	78.0
85	42.5
86	7.0
87	4.5
88	2.0
89	1.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1396664.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.75543265221121
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.56033091821077	13.498231991399287
2	7.50938423573405	2.5164596364283685
3	2.6180430088437414	1.315993299458211
4	1.2815376568497274	0.8589087160247267
5	0.7823325801627257	0.6554160429273589
6	0.4804775510677356	0.4830365548688889
7	0.36892397860939985	0.4327036614162926
8	0.2999023933037057	0.40199954825897594
9	0.22821445797716533	0.344144878280755
>10	2.923335868375282	11.91215385056762
>50	1.2479199992706687	15.237294549066826
>100	1.6901336356408034	50.57720245114429
>500	0.007743040326173189	0.7761935677446551
>1k	0.001290506721028865	0.5389892747325221
>5k	4.3016890700962163E-4	0.45127197768124105
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	6261	0.448282478820962	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4887	0.34990520268296454	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02949886300498903	0.0
2	0.0	0.0	0.0	0.0964440982226219	0.0
3	0.0	0.0	0.0	0.13711243362755823	0.0
4	0.0	0.0	0.0	0.22403384063740456	0.0
5	0.0	0.0	0.0	0.36916538265466853	0.0
6	0.0	0.0	0.0	0.5138673295796269	0.0
7	0.0	0.0	0.0	0.6015763275920336	0.0
8	0.0	0.0	0.0	0.8186650475704965	0.0
9	0.0	0.0	0.0	0.8851806876958238	0.0
10	0.0	0.0	0.0	1.0104076571029252	0.0
11	0.0	0.0	0.0	1.168785047799614	0.0
12	0.0	0.0	0.0	1.2982363689477212	0.0
13	7.159918205094426E-5	0.0	0.0	1.349644581660299	0.0
14	7.159918205094426E-5	0.0	0.0	1.3689047616320031	0.0
15	7.159918205094426E-5	0.0	0.0	1.4029859722882525	0.0
16	1.431983641018885E-4	0.0	0.0	1.4819598700904442	0.0
17	1.431983641018885E-4	0.0	0.0	1.5729624304771943	0.0
18	1.431983641018885E-4	0.0	0.0	1.7086428804637337	0.0
19	1.431983641018885E-4	0.0	0.0	1.7701465778454948	0.0
20	1.431983641018885E-4	0.0	0.0	1.8292159030375237	0.0
21	1.431983641018885E-4	0.0	0.0	1.9156361157730135	0.0
22	1.431983641018885E-4	0.0	0.0	2.006495477795662	0.0
23	1.431983641018885E-4	0.0	0.0	2.125493318364331	0.0
24	1.431983641018885E-4	0.0	0.0	2.2106247458229036	0.0
25	2.1479754615283275E-4	0.0	0.0	2.282080729509746	0.0
26	2.1479754615283275E-4	0.0	0.0	2.3571166722991355	0.0
27	2.1479754615283275E-4	0.0	0.0	2.425780287885991	0.0
28	2.1479754615283275E-4	0.0	0.0	2.5002434372189732	0.0
29	2.1479754615283275E-4	0.0	0.0	2.5805777194801327	0.0
30	2.1479754615283275E-4	0.0	0.0	2.6962819976744585	0.0
31	2.1479754615283275E-4	0.0	0.0	2.7949456705406597	0.0
32	2.86396728203777E-4	0.0	0.0	2.8850174415607475	0.0
33	2.86396728203777E-4	0.0	0.0	2.978024779044924	0.0
34	2.86396728203777E-4	0.0	0.0	3.076903249457278	0.0
35	2.86396728203777E-4	0.0	0.0	3.2101493272540855	0.0
36	2.86396728203777E-4	0.0	0.0	3.3164025134176867	0.0
37	2.86396728203777E-4	0.0	0.0	3.4258060635915295	0.0
38	2.86396728203777E-4	0.0	0.0	3.5353528121294744	0.0
39	2.86396728203777E-4	0.0	0.0	3.6434675770264002	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTCGC	45	3.8562575E-10	45.000004	23
GCTATCG	30	2.165838E-6	45.000004	38
GAACGAT	30	2.165838E-6	45.000004	43
CGTCGCA	30	2.165838E-6	45.000004	45
CGACTGG	45	3.8562575E-10	45.000004	2
CCGTTAT	30	2.165838E-6	45.000004	39
TACACGG	30	2.165838E-6	45.000004	2
GACTAAC	30	2.165838E-6	45.000004	9
TCGCAAA	20	7.033809E-4	45.0	30
CGTTGTA	20	7.033809E-4	45.0	25
GATCCGA	20	7.033809E-4	45.0	44
CGTTATT	20	7.033809E-4	45.0	18
TGCGCAA	20	7.033809E-4	45.0	37
ACGTGTA	20	7.033809E-4	45.0	31
TCGCTAA	20	7.033809E-4	45.0	26
TATTACG	20	7.033809E-4	45.0	1
TTGCGAA	20	7.033809E-4	45.0	28
CTCGCTA	20	7.033809E-4	45.0	25
TCGTACT	20	7.033809E-4	45.0	18
TATAGCG	80	0.0	45.0	1
>>END_MODULE