FastQCFastQC Report
Thu 26 May 2016
SRR1545532_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545532_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1714680
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67840.39564233559614626No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC43640.25450812979681336No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGAT253.8916107E-545.00000426
GTATTCG302.166129E-645.0000049
ATTACGA453.8562575E-1045.00000412
TACGCGC302.166129E-645.00000427
GCGATCG253.8916107E-545.0000049
ACGTATG406.8175723E-945.0000041
TGTAACG700.045.0000041
TAATGCG351.2123928E-745.0000041
AAATGCG551.8189894E-1245.01
CGGGTAT2500.043.1999976
TATAGCG900.042.5000041
CGGTCTA2900.041.8965531
AACGACG603.6379788E-1241.25000413
GATCGAT556.184564E-1140.90909210
CTAAGCG1100.040.9090921
TCACGAC3050.040.57377225
CATAGCG501.0822987E-940.5000041
AGTACGG2000.040.5000042
CGGGATA1450.040.3448266
CGTTTTT51750.040.1304361
Produced by FastQC (version 0.11.5)