FastQCFastQC Report
Thu 26 May 2016
SRR1545531_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545531_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1543841
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57870.37484430067604113No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC45430.2942660546001823No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT16680.10804221419174642No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG15900.10298988043457842No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTCCG302.165987E-645.00000430
GTCGTAC302.165987E-645.00000415
TGTTACG600.045.0000041
TGCGTAT302.165987E-645.00000415
CGTGCAA207.0340396E-445.016
CGTTGTC253.89143E-545.019
ATAGCCG502.1827873E-1145.02
CCAATCG207.0340396E-445.024
CGACACT253.89143E-545.010
CTAACGG800.045.02
CGGATAT207.0340396E-445.011
TCTCGTC207.0340396E-445.026
CGTAACG207.0340396E-445.045
ACGTATG406.8175723E-945.01
GAACCGT207.0340396E-445.038
CGGTCAT207.0340396E-445.023
GCTACGA2050.045.010
CACGTCA253.89143E-545.011
AGAATCG207.0340396E-445.010
AATACGG351.2122837E-745.02