##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545531_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1543841
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.278311691424182	31.0	31.0	33.0	30.0	34.0
2	31.626407123531504	31.0	31.0	34.0	30.0	34.0
3	31.729776576732966	31.0	31.0	34.0	30.0	34.0
4	35.443578062766825	37.0	35.0	37.0	33.0	37.0
5	35.2926907628441	37.0	35.0	37.0	33.0	37.0
6	35.37886673562886	37.0	35.0	37.0	33.0	37.0
7	35.80450771808755	37.0	35.0	37.0	35.0	37.0
8	35.8309851856506	37.0	35.0	37.0	35.0	37.0
9	37.51094899021337	39.0	37.0	39.0	35.0	39.0
10	36.891008206155945	39.0	37.0	39.0	32.0	39.0
11	36.50455390159997	39.0	35.0	39.0	32.0	39.0
12	35.56615869121237	37.0	35.0	39.0	31.0	39.0
13	35.11468797628771	37.0	35.0	39.0	30.0	39.0
14	36.06062930055621	38.0	35.0	40.0	30.0	41.0
15	36.352978059269056	38.0	35.0	40.0	31.0	41.0
16	36.56089584354866	38.0	35.0	40.0	31.0	41.0
17	36.49476338560771	38.0	35.0	40.0	31.0	41.0
18	36.45525089695118	38.0	35.0	40.0	31.0	41.0
19	36.38817663217909	37.0	35.0	40.0	31.0	41.0
20	36.23226355563818	37.0	35.0	40.0	31.0	41.0
21	36.02658369611897	37.0	34.0	40.0	30.0	41.0
22	35.942203892758386	37.0	34.0	40.0	30.0	41.0
23	35.922550314443	36.0	34.0	40.0	30.0	41.0
24	35.866397511142665	36.0	34.0	40.0	30.0	41.0
25	35.772309454147155	36.0	34.0	40.0	30.0	41.0
26	35.66658418839764	36.0	34.0	40.0	30.0	41.0
27	35.585250683198595	36.0	34.0	40.0	30.0	41.0
28	35.582374091632495	36.0	34.0	40.0	30.0	41.0
29	35.60151595922119	36.0	34.0	40.0	30.0	41.0
30	35.50921176468302	36.0	34.0	40.0	29.0	41.0
31	35.330382468142766	36.0	34.0	40.0	29.0	41.0
32	35.14692121792335	36.0	34.0	40.0	29.0	41.0
33	34.97385352507156	36.0	34.0	40.0	27.0	41.0
34	34.82096407596378	36.0	34.0	40.0	27.0	41.0
35	34.690597023916325	36.0	34.0	40.0	26.0	41.0
36	34.49554649734007	35.0	33.0	40.0	25.0	41.0
37	34.41585240967172	35.0	33.0	40.0	24.0	41.0
38	34.41347975601114	35.0	33.0	40.0	25.0	41.0
39	34.36867138520094	35.0	33.0	40.0	24.0	41.0
40	34.18006776604586	35.0	33.0	40.0	23.0	41.0
41	34.19033695827485	35.0	33.0	40.0	24.0	41.0
42	34.10585027862325	35.0	33.0	40.0	24.0	41.0
43	34.007132211153866	35.0	33.0	40.0	23.0	41.0
44	33.94515043971497	35.0	33.0	40.0	23.0	41.0
45	33.89758206965614	35.0	33.0	40.0	23.0	41.0
46	33.86744748973502	35.0	33.0	39.0	23.0	41.0
47	33.82778990841673	35.0	33.0	39.0	23.0	41.0
48	33.78747163729944	35.0	33.0	39.0	23.0	41.0
49	33.752447952865616	35.0	33.0	39.0	24.0	41.0
50	33.62547244178643	35.0	33.0	39.0	24.0	41.0
51	33.46885786813539	35.0	32.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	0.0
11	1.0
12	3.0
13	7.0
14	15.0
15	51.0
16	157.0
17	455.0
18	975.0
19	1956.0
20	3226.0
21	4950.0
22	7312.0
23	10511.0
24	14731.0
25	20239.0
26	26680.0
27	31290.0
28	35014.0
29	39551.0
30	46856.0
31	57039.0
32	70116.0
33	90559.0
34	157495.0
35	237834.0
36	104437.0
37	127954.0
38	174112.0
39	280145.0
40	168.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.593329235329286	23.047192035967434	28.54037430020319	13.819104428500085
2	32.068652147468555	25.683409107544108	29.14820891529633	13.099729829691013
3	29.637313687096018	25.487534014189283	30.592658181768716	14.282494116945982
4	26.922202480695873	27.62493028751018	30.253568858451096	15.19929837334285
5	23.33323185483479	33.553843951546824	28.691685218879403	14.421238974738978
6	20.885894337564554	41.521179966071635	26.78481786660673	10.80810782975708
7	86.45838528708592	4.717649032510472	6.681970487893507	2.1419951925101097
8	87.70689468669377	3.83951456140885	6.480330552174738	1.9732601997226398
9	83.54973083368041	5.517278009846869	7.854371013595313	3.0786201428774076
10	52.8325779662543	22.097741930678094	13.710867893779216	11.358812209288391
11	46.07559975411976	20.627836674890744	19.128329925167165	14.168233645822333
12	42.18834711605664	20.077456162907968	24.102417282608766	13.631779438426625
13	21.94895717888047	39.40580668605122	23.37034707589706	15.274889059171247
14	15.860247266395957	41.829890513336544	27.118530988618645	15.191331231648855
15	15.626673990391499	22.937271389994176	46.839408980588026	14.596645639026299
16	16.959129858579995	17.619754884084564	45.82499104506228	19.596124212273157
17	17.09282238261583	18.406040518421264	29.409958668023457	35.09117843093946
18	21.725488570390343	22.357548478114005	35.008851300101504	20.908111651394155
19	30.070583693528025	22.35353252051215	26.35912636081047	21.216757425149353
20	31.908467257962446	22.489880758445977	25.438500467340873	20.163151516250704
21	22.697998045135474	27.478347835042598	28.45156981839451	21.372084301427414
22	23.115787182747447	23.290869979486228	25.139570720041764	28.453772117724558
23	19.48257624975629	29.569884463490737	25.44504259182131	25.502496694931665
24	19.436004096276754	23.761838168567877	39.9535962576457	16.848561477509666
25	17.720801559227926	24.540480528759115	35.86645256862591	21.872265343387046
26	18.220334866090486	33.252323263859424	26.936970840909137	21.590371029140954
27	18.966266603879546	33.53901081782386	27.531656433531694	19.96306614476491
28	15.479767670375383	28.18496205243934	37.373602592494954	18.96166768469033
29	17.07248350056774	23.977987370461076	36.47415763669963	22.47537149227155
30	18.747008273520393	31.267533379408892	30.69344576287325	19.292012584197465
31	29.559391154918156	26.309704172903814	24.77677429217128	19.354130380006747
32	30.30797860660521	24.97744262524444	26.66777213456567	18.046806633584676
33	28.53162987639271	26.90141018407984	23.971898660548593	20.595061278978857
34	18.997228341519627	27.127469732958254	28.094667779907383	25.780634145614734
35	21.65436725673175	25.09299856656223	30.968797952638905	22.283836224067116
36	31.87342478921081	24.743221614142907	25.023302270117192	18.360051326529092
37	20.607756886881486	33.75503047269764	27.530749604395787	18.106463036025083
38	21.720436236633176	33.37798387269155	22.987989048094978	21.91359084258029
39	21.828284130295803	31.24946157020056	26.311647378195037	20.610606921308605
40	24.242133743047372	26.524104490034922	25.527823137227212	23.705938629690493
41	18.391531252246832	24.47713203626539	27.30553211114357	29.825804600344206
42	22.02273420643706	25.2519527593839	23.863662125827727	28.86165090835131
43	21.64983311105224	25.569148636420458	26.233012337410393	26.548005915116907
44	19.909045037669035	29.93974120391932	27.872106000553167	22.279107757858483
45	18.52120781868081	36.90567875836955	22.9543715965569	21.618741826392743
46	21.594710854291343	33.285552074339265	25.516293452499315	19.603443618870077
47	22.152345999361334	27.473878462872797	26.82646723334851	23.547308304417356
48	22.59798774614743	24.614322329825416	31.249914984768505	21.537774939258643
49	21.763575394098226	24.574486621355437	32.2914730208616	21.370464963684732
50	20.636581098701225	33.195775989884964	26.252573937341992	19.91506897407181
51	19.65603970875239	33.482916958417356	24.71744175727941	22.14360157555085
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1162.0
1	2229.0
2	3296.0
3	7057.0
4	10818.0
5	8387.5
6	5957.0
7	5961.5
8	5966.0
9	6204.5
10	6443.0
11	6503.0
12	6563.0
13	6756.0
14	6949.0
15	6628.5
16	6308.0
17	6212.5
18	6117.0
19	5542.0
20	4967.0
21	5180.0
22	5393.0
23	5721.0
24	6049.0
25	6812.5
26	9902.5
27	12229.0
28	13956.0
29	15683.0
30	18796.5
31	21910.0
32	26805.0
33	31700.0
34	36367.5
35	41035.0
36	42237.5
37	43440.0
38	50393.0
39	57346.0
40	80411.5
41	103477.0
42	127271.5
43	151066.0
44	155749.0
45	160432.0
46	151888.0
47	143344.0
48	131399.0
49	119454.0
50	110282.0
51	101110.0
52	95953.0
53	90796.0
54	78690.5
55	66585.0
56	64170.0
57	61755.0
58	56888.0
59	52021.0
60	49508.0
61	46995.0
62	42627.0
63	38259.0
64	34090.5
65	29922.0
66	25978.5
67	22035.0
68	19102.5
69	16170.0
70	14080.0
71	11990.0
72	10432.5
73	8875.0
74	7363.5
75	4414.0
76	2976.0
77	2389.5
78	1803.0
79	1396.5
80	990.0
81	734.5
82	479.0
83	436.0
84	393.0
85	228.0
86	63.0
87	69.5
88	76.0
89	43.0
90	10.0
91	7.0
92	4.0
93	2.5
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1543841.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.776998457367212
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.70593208814188	12.732973660549977
2	6.843526701820762	2.1594062043515496
3	2.385661134151058	1.1291571609990647
4	1.219845155562744	0.7698198055016112
5	0.7050570724738975	0.556184217238826
6	0.47920247191085574	0.4536225996062475
7	0.35903867184762794	0.3965186800312639
8	0.2986626231219753	0.37695997954149196
9	0.2544054203630633	0.3612378532152517
>10	3.3205631583428636	13.210123143405832
>50	1.6818212867992306	19.381817609974664
>100	1.7326478266542065	46.286669599427434
>500	0.010743821486527395	1.0444783425623636
>1k	0.00247934341996786	0.7637515625190743
>5k	4.132239033279767E-4	0.37727958107536347
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5787	0.37484430067604113	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4543	0.2942660546001823	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1668	0.10804221419174642	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1590	0.10298988043457842	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.01593428338799138	0.0
2	0.0	0.0	0.0	0.06023936402777229	0.0
3	0.0	0.0	0.0	0.08919312286692736	0.0
4	0.0	0.0	0.0	0.1647838086953255	0.0
5	0.0	0.0	0.0	0.27528741625594866	0.0
6	0.0	0.0	0.0	0.40146621316573405	0.0
7	0.0	0.0	0.0	0.47459550562525543	0.0
8	6.477350970728204E-5	0.0	0.0	0.6507146785193553	0.0
9	6.477350970728204E-5	0.0	0.0	0.7125086067801023	0.0
10	6.477350970728204E-5	0.0	0.0	0.8081790806177579	0.0
11	6.477350970728204E-5	0.0	0.0	0.9192008762560393	0.0
12	6.477350970728204E-5	0.0	0.0	1.022708944768276	0.0
13	6.477350970728204E-5	0.0	0.0	1.061054862514987	0.0
14	6.477350970728204E-5	0.0	0.0	1.0758879962379546	0.0
15	6.477350970728204E-5	0.0	0.0	1.0981700835772596	0.0
16	6.477350970728204E-5	0.0	0.0	1.1529684727896201	0.0
17	6.477350970728204E-5	0.0	0.0	1.218130623555146	0.0
18	1.943205291218461E-4	0.0	0.0	1.3172988669169947	0.0
19	1.943205291218461E-4	0.0	0.0	1.36102098596941	0.0
20	1.943205291218461E-4	0.0	0.0	1.4059090281965565	0.0
21	1.943205291218461E-4	0.0	0.0	1.4694518412194002	0.0
22	1.943205291218461E-4	0.0	0.0	1.5396015522323867	0.0
23	1.943205291218461E-4	0.0	0.0	1.613184259259859	0.0
24	1.943205291218461E-4	0.0	0.0	1.6746543199720696	0.0
25	1.943205291218461E-4	0.0	0.0	1.7238174138398967	0.0
26	1.943205291218461E-4	0.0	0.0	1.7725270931397727	0.0
27	1.943205291218461E-4	0.0	0.0	1.8154719300757007	0.0
28	1.943205291218461E-4	0.0	0.0	1.8703350927977687	0.0
29	2.5909403882912814E-4	0.0	0.0	1.9275301018692987	0.0
30	2.5909403882912814E-4	0.0	0.0	2.002667373129746	0.0
31	2.5909403882912814E-4	0.0	0.0	2.0745011953951216	0.0
32	3.238675485364102E-4	0.0	0.0	2.137007632262649	0.0
33	3.238675485364102E-4	0.0	0.0	2.204825496926173	0.0
34	3.238675485364102E-4	0.0	0.0	2.2789263920313036	0.0
35	4.5341456795097425E-4	0.0	0.0	2.3804264817426146	0.0
36	4.5341456795097425E-4	0.0	0.0	2.4628183860902775	0.0
37	4.5341456795097425E-4	0.0	0.0	2.542230708991405	0.0
38	4.5341456795097425E-4	0.0	0.0	2.62339191665463	0.0
39	4.5341456795097425E-4	0.0	0.0	2.710512287210924	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTCCG	30	2.165987E-6	45.000004	30
GTCGTAC	30	2.165987E-6	45.000004	15
TGTTACG	60	0.0	45.000004	1
TGCGTAT	30	2.165987E-6	45.000004	15
CGTGCAA	20	7.0340396E-4	45.0	16
CGTTGTC	25	3.89143E-5	45.0	19
ATAGCCG	50	2.1827873E-11	45.0	2
CCAATCG	20	7.0340396E-4	45.0	24
CGACACT	25	3.89143E-5	45.0	10
CTAACGG	80	0.0	45.0	2
CGGATAT	20	7.0340396E-4	45.0	11
TCTCGTC	20	7.0340396E-4	45.0	26
CGTAACG	20	7.0340396E-4	45.0	45
ACGTATG	40	6.8175723E-9	45.0	1
GAACCGT	20	7.0340396E-4	45.0	38
CGGTCAT	20	7.0340396E-4	45.0	23
GCTACGA	205	0.0	45.0	10
CACGTCA	25	3.89143E-5	45.0	11
AGAATCG	20	7.0340396E-4	45.0	10
AATACGG	35	1.2122837E-7	45.0	2
>>END_MODULE