FastQCFastQC Report
Thu 26 May 2016
SRR1545530_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545530_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2568338
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130650.5086947278746021No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC46750.18202432857357562No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACGA406.8212103E-945.0000048
TAACGCG207.0349145E-445.0000041
GCGATCG207.0349145E-445.0000049
ACTCGAC253.8921557E-545.040
TAATACG1800.041.2500041
CGTTTTT87300.040.6185531
ACTAACG501.0822987E-940.51
CGGTCTA2900.040.3448331
GCGATAC950.040.2631579
TGCACGA451.9299478E-840.00000436
GGGCGAT37050.039.8380557
CGTAAGG4250.039.705882
TACGTAG1850.038.918921
AGTACGG3600.038.7500042
AGGGCGA20850.038.741016
GTCGATC356.2513027E-638.5714269
CGACGGT3050.038.36065728
ACGTAAG659.094947E-1238.0769271
TAACGGG6150.038.048783
TGGGCGA13150.037.984796
Produced by FastQC (version 0.11.5)