##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545530_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2568338
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.302457464710642	31.0	31.0	33.0	30.0	34.0
2	31.657596079643724	31.0	31.0	34.0	30.0	34.0
3	31.75000214146269	31.0	31.0	34.0	30.0	34.0
4	35.46099929214924	37.0	35.0	37.0	33.0	37.0
5	35.31919163287698	37.0	35.0	37.0	33.0	37.0
6	35.39516449937664	37.0	35.0	37.0	33.0	37.0
7	35.85048541118809	37.0	35.0	37.0	35.0	37.0
8	35.87679931535491	37.0	35.0	37.0	35.0	37.0
9	37.572678518170115	39.0	37.0	39.0	35.0	39.0
10	36.89920758093366	39.0	37.0	39.0	32.0	39.0
11	36.526661210479304	39.0	35.0	39.0	32.0	39.0
12	35.92046179280141	37.0	35.0	39.0	31.0	39.0
13	35.642206750046135	37.0	35.0	39.0	30.0	39.0
14	36.62867621006269	38.0	35.0	41.0	31.0	41.0
15	36.81795269937212	38.0	35.0	41.0	31.0	41.0
16	36.938502642564956	38.0	35.0	41.0	32.0	41.0
17	36.880490807674065	38.0	35.0	41.0	32.0	41.0
18	36.799757664294965	38.0	35.0	40.0	31.0	41.0
19	36.745310780746145	38.0	35.0	40.0	31.0	41.0
20	36.617203810401904	38.0	35.0	40.0	31.0	41.0
21	36.43943164801517	38.0	35.0	40.0	31.0	41.0
22	36.37568575475658	38.0	35.0	40.0	30.0	41.0
23	36.31429001946005	38.0	35.0	40.0	30.0	41.0
24	36.23780748484039	38.0	35.0	40.0	30.0	41.0
25	36.12915005735226	38.0	35.0	40.0	30.0	41.0
26	36.035276120199136	38.0	35.0	40.0	30.0	41.0
27	35.99557145515894	38.0	34.0	40.0	30.0	41.0
28	35.95915763423662	38.0	35.0	40.0	30.0	41.0
29	35.92250825241849	38.0	35.0	40.0	30.0	41.0
30	35.802397114398495	37.0	34.0	40.0	30.0	41.0
31	35.639346923964055	37.0	34.0	40.0	29.0	41.0
32	35.47300277455693	37.0	34.0	40.0	29.0	41.0
33	35.31276568738227	37.0	34.0	40.0	27.0	41.0
34	35.12690035345815	37.0	34.0	40.0	27.0	41.0
35	34.97640108116611	37.0	34.0	40.0	26.0	41.0
36	34.830607965151	37.0	34.0	40.0	25.0	41.0
37	34.76835642349255	37.0	33.0	40.0	25.0	41.0
38	34.724692388618635	37.0	33.0	40.0	25.0	41.0
39	34.63776224157412	37.0	33.0	40.0	24.0	41.0
40	34.478055847789506	37.0	33.0	40.0	24.0	41.0
41	34.4181824977865	36.0	33.0	40.0	24.0	41.0
42	34.334902960591634	36.0	33.0	40.0	23.0	41.0
43	34.24623083098876	36.0	33.0	40.0	23.0	41.0
44	34.182194866875	36.0	33.0	40.0	23.0	41.0
45	34.132442069540694	36.0	33.0	40.0	23.0	41.0
46	34.09709469703754	35.0	33.0	40.0	23.0	41.0
47	34.03313310008262	35.0	33.0	40.0	23.0	41.0
48	33.98449035913497	35.0	33.0	40.0	23.0	41.0
49	33.921362764558246	36.0	33.0	40.0	24.0	41.0
50	33.806629423385864	35.0	33.0	40.0	23.0	41.0
51	33.66147485260896	35.0	33.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	6.0
13	10.0
14	28.0
15	99.0
16	323.0
17	795.0
18	1729.0
19	3294.0
20	5586.0
21	8609.0
22	12630.0
23	17925.0
24	24642.0
25	33288.0
26	42791.0
27	50063.0
28	55904.0
29	62748.0
30	74004.0
31	88821.0
32	108939.0
33	138720.0
34	220993.0
35	316643.0
36	192576.0
37	237205.0
38	331454.0
39	538285.0
40	225.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.34568892412136	22.314508448654344	29.235248631605344	15.104553995618957
2	32.05446479396403	25.65410004446455	28.83818251336078	13.453252648210634
3	29.051666875621514	25.347092166217998	31.843199765762918	13.758041192397574
4	25.873697309310533	28.032992542258846	31.52610754503496	14.56720260339566
5	23.403228079793237	32.542640415708526	30.133339147729	13.92079235676924
6	21.02963083519381	42.04158486928122	26.653695892051594	10.275088403473374
7	87.07841413396523	4.055813526101315	7.139325119980314	1.726447219953137
8	88.0158686278831	3.3006169748685728	6.965516220995835	1.7179981762525025
9	83.35347606117264	5.179380595544667	8.456324673777361	3.010818669505338
10	45.90026702092948	26.007207774054663	15.800023205668412	12.292501999347438
11	37.54069752501423	23.072547304910803	24.111546065977297	15.27520910409767
12	35.27514680700126	21.20269995615842	27.723259166044343	15.798894070795978
13	22.545085576742625	32.955202936685126	27.26868504067611	17.231026445896138
14	18.293620232228	34.38021008138337	30.0579985967579	17.268171089630727
15	18.044899074810246	23.977490501639583	41.27934095901708	16.698269464533094
16	20.299314186839894	20.7204425585729	39.74106990590802	19.239173348679184
17	20.26162444351172	20.922674507794532	29.650770264661425	29.16493078403232
18	22.216429457493522	23.26909464408501	34.450566864641644	20.063909033779822
19	26.774318644975857	24.974438722629184	27.73988470364882	20.511357928746136
20	29.155469412515018	24.06042351123567	27.137160295880058	19.64694678036925
21	23.809560891128815	26.34536420050632	30.041723480320737	19.803351428044127
22	23.05331307639415	22.89317060293466	28.955924025576074	25.09759229509512
23	20.87688614193303	27.81837904512568	27.73416115791613	23.570573655025157
24	20.062935641648412	24.259268055840003	36.74010196477255	18.937694337739035
25	20.105531281318893	25.48648191943584	32.64017430727576	21.767812491969515
26	19.601236285878258	30.615168252776698	28.509020230203348	21.274575231141696
27	18.88474180579036	30.284059185356444	29.927174694296465	20.90402431455673
28	17.8401752417322	27.337912689061955	34.67954762963441	20.142364439571427
29	19.578809331170586	23.92274692816911	34.48152073442047	22.016923006239832
30	20.414914236366087	28.665580620619245	31.316127394447303	19.60337774856736
31	26.454695604706234	26.51333274670234	27.366374674984367	19.665596973607055
32	26.775214165736756	25.699654796214517	28.026490282821033	19.4986407552277
33	26.12304143769239	26.77708307862906	26.656888618242615	20.442986865435937
34	20.57930069951852	26.937731715996883	29.262659354025832	23.220308230458762
35	20.890980859995842	26.523572831924767	30.912364338338644	21.673081969740743
36	27.161261485053757	25.899862089802824	27.168931815049262	19.769944610094154
37	20.74547820419275	30.477647412451166	28.858351198323582	19.918523185032498
38	21.92110228482388	30.152495504875137	26.358368719381954	21.56803349091903
39	20.883972436649692	30.32334529178013	27.216121865579996	21.57656040599018
40	24.033246402926718	25.976370711331608	27.795368055139157	22.195014830602513
41	19.743040051581996	25.291102650819326	28.252589807104826	26.713267490493852
42	21.77271838831182	25.906247542184868	26.91152799981934	25.409506069683975
43	21.4814794625941	25.894216415440646	28.087424630247266	24.536879491717993
44	20.5489698007038	28.013875120797966	29.756052357594676	21.68110272090356
45	19.03456632265691	32.929116027563346	25.616643915247916	22.419673734531827
46	21.946799837093092	30.29270290748336	27.372643320310647	20.3878539351129
47	21.62137537972027	27.659326770853372	27.860390649517313	22.858907199909044
48	22.18668259395765	26.635785476833657	29.75546832231583	21.422063606892863
49	21.70064843490226	25.531764121389006	30.825888181384226	21.94169926232451
50	20.13422688135284	30.397595643564046	28.229306267321512	21.238871207761594
51	19.80557076210374	31.31737333637551	26.837160840979653	22.0398950605411
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2346.0
1	4012.5
2	5679.0
3	14534.0
4	23389.0
5	17252.5
6	11116.0
7	11258.5
8	11401.0
9	12132.0
10	12863.0
11	13076.0
12	13289.0
13	13372.0
14	13455.0
15	12764.0
16	12073.0
17	11947.0
18	11821.0
19	11829.0
20	11837.0
21	12279.0
22	12721.0
23	13226.0
24	13731.0
25	16042.0
26	21841.0
27	25329.0
28	31579.5
29	37830.0
30	43350.0
31	48870.0
32	55425.5
33	61981.0
34	72575.5
35	83170.0
36	90451.0
37	97732.0
38	99091.5
39	100451.0
40	127740.0
41	155029.0
42	175441.5
43	195854.0
44	203915.0
45	211976.0
46	209234.0
47	206492.0
48	196248.5
49	186005.0
50	179715.5
51	173426.0
52	161024.5
53	148623.0
54	137952.0
55	127281.0
56	121046.5
57	114812.0
58	106985.0
59	99158.0
60	91583.5
61	84009.0
62	75831.5
63	67654.0
64	59148.0
65	50642.0
66	45808.0
67	40974.0
68	33748.0
69	26522.0
70	22451.5
71	18381.0
72	15617.0
73	12853.0
74	10847.5
75	7067.5
76	5293.0
77	3930.5
78	2568.0
79	1741.5
80	915.0
81	860.5
82	806.0
83	606.0
84	406.0
85	279.0
86	152.0
87	149.0
88	146.0
89	85.0
90	24.0
91	38.0
92	52.0
93	27.5
94	3.0
95	2.5
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2568338.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.796657598240415
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.03094127920723	13.442523179317286
2	6.6699044010263275	2.2406420087407213
3	2.4101121584606213	1.2144548609705763
4	1.4149512838216347	0.9506580893017078
5	0.9375158632302183	0.7873566473798385
6	0.6622657195184234	0.667431031788197
7	0.4913594127298776	0.5777237069307374
8	0.3517234747689734	0.472622301996624
9	0.320355744500996	0.4842815175011359
>10	3.580449807976221	14.734374888539723
>50	1.5726154954444804	19.148123757146816
>100	1.5491560799387496	43.52519126311272
>500	0.0077142221463923875	0.9023395499608813
>1k	7.01292922399308E-4	0.33928532488629626
>5k	0.0	0.0
>10k+	2.3376430746643599E-4	0.51299187242674
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13065	0.5086947278746021	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4675	0.18202432857357562	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.893568525637981E-5	0.0	0.0	0.0123426122262724	0.0
2	3.893568525637981E-5	0.0	0.0	0.05820884945828781	0.0
3	3.893568525637981E-5	0.0	0.0	0.09083695370313409	0.0
4	3.893568525637981E-5	0.0	0.0	0.14573626991462962	0.0
5	3.893568525637981E-5	0.0	0.0	0.2639450103529987	0.0
6	3.893568525637981E-5	0.0	0.0	0.38032377358431796	0.0
7	3.893568525637981E-5	0.0	0.0	0.4486948368945209	0.0
8	7.787137051275962E-5	0.0	0.0	0.597857447111712	0.0
9	7.787137051275962E-5	0.0	0.0	0.6589086015937154	0.0
10	7.787137051275962E-5	0.0	0.0	0.7703425327974744	0.0
11	7.787137051275962E-5	0.0	0.0	0.9298620352928626	0.0
12	1.1680705576913942E-4	0.0	0.0	1.056325141005584	0.0
13	1.5574274102551924E-4	0.0	0.0	1.1062406895042631	0.0
14	1.5574274102551924E-4	0.0	0.0	1.1277331877657848	0.0
15	1.5574274102551924E-4	0.0	0.0	1.1620744621619117	0.0
16	1.5574274102551924E-4	0.0	0.0	1.2347673865355728	0.0
17	1.5574274102551924E-4	0.0	0.0	1.3176614604464054	0.0
18	1.5574274102551924E-4	0.0	0.0	1.4229435533796564	0.0
19	1.5574274102551924E-4	0.0	0.0	1.4808409173558932	0.0
20	1.5574274102551924E-4	0.0	0.0	1.543760984730203	0.0
21	1.5574274102551924E-4	0.0	0.0	1.6213987411314243	0.0
22	1.5574274102551924E-4	0.0	0.0	1.7033194229108475	0.0
23	1.9467842628189904E-4	0.0	0.0	1.7973491028050046	0.0
24	1.9467842628189904E-4	0.0	0.0	1.875103666261995	0.0
25	1.9467842628189904E-4	0.0	0.0	1.9352982356683583	0.0
26	1.9467842628189904E-4	0.0	0.0	1.9962325830945926	0.0
27	1.9467842628189904E-4	0.0	0.0	2.066667237723384	0.0
28	1.9467842628189904E-4	0.0	0.0	2.1335587449938442	0.0
29	1.9467842628189904E-4	0.0	0.0	2.214739648753396	0.0
30	1.9467842628189904E-4	0.0	0.0	2.3079516792571693	0.0
31	2.3361411153827885E-4	0.0	0.0	2.386952184642364	0.0
32	2.3361411153827885E-4	0.0	0.0	2.4670818248999935	0.0
33	2.3361411153827885E-4	0.0	0.0	2.5527792681492856	0.0
34	2.3361411153827885E-4	0.0	0.0	2.646030234338315	0.0
35	2.7254979679465865E-4	0.0	0.0	2.7590994643228424	0.0
36	2.7254979679465865E-4	0.0	0.0	2.851649588177257	0.0
37	2.7254979679465865E-4	0.0	0.0	2.949339222485514	0.0
38	2.7254979679465865E-4	0.0	0.0	3.059760825872607	0.0
39	2.7254979679465865E-4	0.0	0.0	3.2193192640532518	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTACGA	40	6.8212103E-9	45.000004	8
TAACGCG	20	7.0349145E-4	45.000004	1
GCGATCG	20	7.0349145E-4	45.000004	9
ACTCGAC	25	3.8921557E-5	45.0	40
TAATACG	180	0.0	41.250004	1
CGTTTTT	8730	0.0	40.618553	1
ACTAACG	50	1.0822987E-9	40.5	1
CGGTCTA	290	0.0	40.34483	31
GCGATAC	95	0.0	40.263157	9
TGCACGA	45	1.9299478E-8	40.000004	36
GGGCGAT	3705	0.0	39.838055	7
CGTAAGG	425	0.0	39.70588	2
TACGTAG	185	0.0	38.91892	1
AGTACGG	360	0.0	38.750004	2
AGGGCGA	2085	0.0	38.74101	6
GTCGATC	35	6.2513027E-6	38.571426	9
CGACGGT	305	0.0	38.360657	28
ACGTAAG	65	9.094947E-12	38.076927	1
TAACGGG	615	0.0	38.04878	3
TGGGCGA	1315	0.0	37.98479	6
>>END_MODULE