FastQCFastQC Report
Thu 26 May 2016
SRR1545529_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545529_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences531280
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39390.7414169552778196No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC18420.34670983285649754No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG9490.17862520704713147No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6590.1240400542087035No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG5860.11029965366661648No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT5430.10220599307333234No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCTTA253.887964E-545.040
CTCGTTA207.0298585E-445.017
CGTATTC207.0298585E-445.022
ACGTTAG207.0298585E-445.01
CAACCGG207.0298585E-445.041
TACGGGT551.8189894E-1245.04
TACGCGG207.0298585E-445.02
TCGTAAG207.0298585E-445.01
CTATTAC207.0298585E-445.013
ACGTATT207.0298585E-445.021
CGGTCTA453.8380676E-1045.031
ATAACGG502.1827873E-1145.02
CGCACAG253.887964E-545.043
GATTCGT253.887964E-545.09
GCGTATG453.8380676E-1045.01
ACGACTA207.0298585E-445.012
GTCGGAT207.0298585E-445.019
TACGGGC302.1632895E-644.9999964
CTCGAGG600.044.9999962
AAGGGTA2050.042.804885