##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545529_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	531280
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.943845053455806	31.0	31.0	34.0	31.0	34.0
2	32.34917934046077	33.0	31.0	34.0	31.0	34.0
3	32.43362445414847	34.0	31.0	34.0	31.0	34.0
4	36.0879630326758	37.0	35.0	37.0	35.0	37.0
5	36.03054886312302	37.0	35.0	37.0	35.0	37.0
6	36.101389098027404	37.0	35.0	37.0	35.0	37.0
7	36.24691499774131	37.0	35.0	37.0	35.0	37.0
8	36.25318476133113	37.0	36.0	37.0	35.0	37.0
9	38.0056580334287	39.0	39.0	39.0	35.0	39.0
10	37.682001957536514	39.0	37.0	39.0	35.0	39.0
11	37.4221803945189	39.0	37.0	39.0	35.0	39.0
12	36.73386914621292	39.0	35.0	39.0	33.0	39.0
13	36.48557257943081	39.0	35.0	39.0	33.0	39.0
14	37.61483963258545	40.0	35.0	41.0	33.0	41.0
15	37.741191085679866	40.0	35.0	41.0	33.0	41.0
16	37.864192139737995	40.0	35.0	41.0	34.0	41.0
17	37.82504705616624	40.0	35.0	41.0	34.0	41.0
18	37.73833571751242	39.0	36.0	41.0	34.0	41.0
19	37.652367866285196	39.0	36.0	41.0	34.0	41.0
20	37.485555639210965	39.0	35.0	41.0	34.0	41.0
21	37.33487991266376	39.0	35.0	41.0	33.0	41.0
22	37.27559667218792	39.0	35.0	41.0	33.0	41.0
23	37.224013702755606	38.0	35.0	41.0	33.0	41.0
24	37.14180281584099	38.0	35.0	41.0	33.0	41.0
25	37.03859170305677	38.0	35.0	41.0	33.0	41.0
26	36.978340987803044	38.0	35.0	41.0	33.0	41.0
27	36.95932464990212	38.0	35.0	41.0	33.0	41.0
28	36.93588691462129	38.0	35.0	41.0	33.0	41.0
29	36.90786402650203	38.0	35.0	41.0	33.0	41.0
30	36.80736334889324	38.0	35.0	41.0	33.0	41.0
31	36.622656602921246	38.0	35.0	41.0	32.0	41.0
32	36.3839519650655	38.0	35.0	41.0	32.0	41.0
33	36.18555187471766	38.0	35.0	41.0	31.0	41.0
34	35.9610883150128	38.0	35.0	41.0	31.0	41.0
35	35.76660894443608	38.0	35.0	40.0	30.0	41.0
36	35.59761519349495	38.0	35.0	40.0	30.0	41.0
37	35.52545362144255	38.0	35.0	40.0	29.0	41.0
38	35.50383037193194	38.0	35.0	40.0	29.0	41.0
39	35.44984565577473	37.0	35.0	40.0	29.0	41.0
40	35.27908823972293	37.0	35.0	40.0	28.0	41.0
41	35.25735770215329	37.0	35.0	40.0	29.0	41.0
42	35.1757058424936	37.0	35.0	40.0	28.0	41.0
43	35.0887027556091	37.0	35.0	40.0	28.0	41.0
44	34.99455654268935	36.0	34.0	40.0	27.0	41.0
45	34.943925990061736	36.0	34.0	40.0	27.0	41.0
46	34.919590423129044	36.0	34.0	40.0	27.0	41.0
47	34.88250451739196	36.0	34.0	40.0	27.0	41.0
48	34.82415110676103	36.0	34.0	40.0	27.0	41.0
49	34.763331953019126	36.0	34.0	40.0	27.0	41.0
50	34.64694699593435	36.0	34.0	40.0	27.0	41.0
51	34.506288586056314	35.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	1.0
14	3.0
15	14.0
16	21.0
17	63.0
18	123.0
19	281.0
20	513.0
21	758.0
22	1282.0
23	1956.0
24	3175.0
25	5148.0
26	7037.0
27	8295.0
28	8066.0
29	8192.0
30	9068.0
31	11041.0
32	14625.0
33	21113.0
34	44981.0
35	85401.0
36	35291.0
37	46956.0
38	74481.0
39	143297.0
40	97.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.71401144405963	22.982231591627766	28.334776389098025	15.968980575214575
2	32.75937358831501	25.48731365758169	28.48177985243186	13.271532901671435
3	28.737012498117753	24.500451739195906	33.18193796115043	13.580597801535912
4	26.863047733775037	27.020591778346635	31.387592230085833	14.728768257792503
5	22.472707423580786	32.13691462129197	31.379686794157507	14.010691160969731
6	20.655586508056015	41.856271645836465	27.555338051498268	9.932803794609246
7	84.90984038548412	4.700158108718567	8.698614666465893	1.6913868393314258
8	85.95580484866737	3.733436229483512	8.669816292726999	1.64094262912212
9	81.641507303117	5.792237614817046	9.971013401596144	2.5952416804698086
10	47.83711037494353	24.33330823671134	15.988555940370427	11.841025447974703
11	41.3768634241831	21.267504893841288	22.555714500828188	14.799917181147418
12	37.57660743863876	21.058951965065503	27.9641620237916	13.40027857250414
13	21.350888420418613	36.42504893841289	26.21630778497214	16.007754856196357
14	14.854690558650804	39.744014455654266	29.714463183255535	15.686831802439391
15	15.501806956783618	23.261180545098632	46.73863123023641	14.498381267881344
16	16.58485167896401	18.61410179189881	45.28911308537871	19.51193344375847
17	16.762535762686344	19.04438337599759	31.006813732871557	33.18626712844451
18	20.776238518295436	23.659652160819153	35.9471465140792	19.616962806806203
19	27.432615569944286	24.20305676855895	28.0499924710134	20.31433519048336
20	30.39207197711188	22.272436380063244	28.038134317120917	19.29735732570396
21	22.173994880289115	25.717700647492848	29.97929528685439	22.12900918536365
22	22.067271495256737	24.033842794759824	26.710962204487277	27.18792350549616
23	19.689617527480802	27.87042614064147	27.427345279325404	25.012611052552323
24	19.47184159012197	23.433029664207197	39.70524017467249	17.389888570998345
25	17.858003312754104	25.14248607137479	35.9051724137931	21.094338202078
26	18.345128745670834	32.42508658334588	28.466721879235056	20.76306279174823
27	18.24499322391206	32.75335039903629	29.23938412889625	19.762272248155398
28	15.965404306580332	28.67998042463484	36.67275259750038	18.681862671284442
29	16.83123776539678	24.577623851829543	36.452153290167146	22.138985092606536
30	18.59113838277368	30.521946995934346	32.546679716910106	18.34023490438187
31	27.84972142749586	27.609170305676855	26.33168950459268	18.209418762234602
32	27.405511218190032	26.486786628519805	28.163303719319376	17.944398433970786
33	26.65185965968981	29.04061888269839	25.29475982532751	19.012761632284295
34	20.016187321186568	29.113273603372985	27.666202379159767	23.20433669628068
35	21.683293178738143	26.489233549164283	30.377955127239876	21.449518144857702
36	27.959832856497513	27.284106309290767	26.179227525975	18.57683330823671
37	20.644857702153292	33.20828941424484	28.755458515283845	17.391394368318025
38	20.476396627014005	32.382359584399936	25.337110374943535	21.804133413642525
39	21.737690106911607	30.753651558500227	27.848403854841138	19.660254479747024
40	23.447522963409124	27.05748381267881	27.44560307182653	22.04939015208553
41	17.777066706821262	26.515208552928776	29.53433217888872	26.17339256136124
42	22.407769914169553	26.767617828640265	26.166051799427798	24.658560457762384
43	22.38292425839482	26.397944586658635	27.933669628068063	23.285461526878482
44	19.054547507905436	30.318852582442403	29.33613160668574	21.29046830296642
45	17.813017617828642	36.56847613311248	24.720109923204337	20.89839632585454
46	21.307220298147868	33.052062942327964	26.95113687697636	18.68957988254781
47	22.270554133413643	29.03854841138383	27.365607589218495	21.325289865984036
48	22.20768709531697	26.65562415298901	29.897417557596746	21.239271194097277
49	20.612106610450233	27.246461376298754	31.302514681523867	20.83891733172715
50	20.031809968378255	33.04491040505948	27.75391507303117	19.169364553531096
51	18.94537720222858	32.561173016112036	26.295173919590425	22.198275862068968
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	919.0
1	1432.0
2	1945.0
3	4579.5
4	7214.0
5	5215.0
6	3216.0
7	3299.0
8	3382.0
9	3656.5
10	3931.0
11	3860.0
12	3789.0
13	3724.5
14	3660.0
15	3667.5
16	3675.0
17	3400.0
18	3125.0
19	3078.0
20	3031.0
21	2978.0
22	2925.0
23	3024.0
24	3123.0
25	3537.0
26	4917.5
27	5884.0
28	6500.0
29	7116.0
30	8064.5
31	9013.0
32	10475.0
33	11937.0
34	13485.0
35	15033.0
36	16027.0
37	17021.0
38	19696.5
39	22372.0
40	27238.5
41	32105.0
42	38984.5
43	45864.0
44	46995.0
45	48126.0
46	47705.0
47	47284.0
48	42373.0
49	37462.0
50	35722.5
51	33983.0
52	29971.5
53	25960.0
54	24116.0
55	22272.0
56	21106.5
57	19941.0
58	19712.0
59	19483.0
60	18499.0
61	17515.0
62	15970.5
63	14426.0
64	12018.0
65	9610.0
66	8015.5
67	6421.0
68	5667.5
69	4914.0
70	4359.0
71	3804.0
72	3113.0
73	2422.0
74	1963.0
75	1138.5
76	773.0
77	676.5
78	580.0
79	421.0
80	262.0
81	218.0
82	174.0
83	129.5
84	85.0
85	52.0
86	19.0
87	17.0
88	15.0
89	10.5
90	6.0
91	5.5
92	5.0
93	4.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	531280.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.040260136344106
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.29641189037699	13.76542489317059
2	5.849143575185872	2.227384304927296
3	2.672129478542512	1.5263412116832884
4	1.6715416307885798	1.2730634991577365
5	1.2286352278970116	1.1696767175917764
6	1.0426887728759977	1.1911839286082648
7	0.9387197010302093	1.2511427111908446
8	0.8287525303897864	1.2623731013819965
9	0.7337808889868578	1.2574241108448847
>10	10.721798411521936	46.912236072918574
>50	1.9114292366271304	23.853940681258404
>100	0.09897044687556794	2.6884435574980143
>500	0.003998805934366381	0.5209763535026951
>1k	0.0019994029671831907	1.1003888562656488
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3939	0.7414169552778196	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1842	0.34670983285649754	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	949	0.17862520704713147	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	659	0.1240400542087035	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	586	0.11029965366661648	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	543	0.10220599307333234	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.00828188525824424	0.0
2	0.0	0.0	0.0	0.0570320734829092	0.0
3	0.0	0.0	0.0	0.08056015660292125	0.0
4	0.0	0.0	0.0	0.1197108869146213	0.0
5	0.0	0.0	0.0	0.19142448426441802	0.0
6	0.0	0.0	0.0	0.263702755609095	0.0
7	0.0	0.0	0.0	0.30454750790543594	0.0
8	0.0	0.0	0.0	0.37946092455955427	0.0
9	0.0	0.0	0.0	0.4169176328866135	0.0
10	0.0	0.0	0.0	0.48392561361240777	0.0
11	0.0	0.0	0.0	0.5851904833609396	0.0
12	0.0	0.0	0.0	0.6665035386237013	0.0
13	0.0	0.0	0.0	0.7028308989609998	0.0
14	0.0	0.0	0.0	0.7171359734979672	0.0
15	0.0	0.0	0.0	0.7387818099683783	0.0
16	0.0	0.0	0.0	0.789037795512724	0.0
17	0.0	0.0	0.0	0.8487050143050745	0.0
18	0.0	0.0	0.0	0.9191010390001506	0.0
19	0.0	0.0	0.0	0.9620162626110526	0.0
20	0.0	0.0	0.0	1.0030492395723536	0.0
21	0.0	0.0	0.0	1.0636575816895046	0.0
22	0.0	0.0	0.0	1.1206896551724137	0.0
23	0.0	0.0	0.0	1.193156151182051	0.0
24	0.0	0.0	0.0	1.2475530793555187	0.0
25	0.0	0.0	0.0	1.2921623249510616	0.0
26	0.0	0.0	0.0	1.3362068965517242	0.0
27	0.0	0.0	0.0	1.378557446167746	0.0
28	0.0	0.0	0.0	1.4184610751392863	0.0
29	0.0	0.0	0.0	1.468905285348592	0.0
30	0.0	0.0	0.0	1.535348592079506	0.0
31	0.0	0.0	0.0	1.5965216081915374	0.0
32	0.0	0.0	0.0	1.6593886462882097	0.0
33	0.0	0.0	0.0	1.7209381117301612	0.0
34	0.0	0.0	0.0	1.7868167444661949	0.0
35	0.0	0.0	0.0	1.87528233699744	0.0
36	0.0	0.0	0.0	1.9503839783165187	0.0
37	0.0	0.0	0.0	2.028873663604879	0.0
38	0.0	0.0	0.0	2.097575666315314	0.0
39	0.0	0.0	0.0	2.1730537569643125	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCCTTA	25	3.887964E-5	45.0	40
CTCGTTA	20	7.0298585E-4	45.0	17
CGTATTC	20	7.0298585E-4	45.0	22
ACGTTAG	20	7.0298585E-4	45.0	1
CAACCGG	20	7.0298585E-4	45.0	41
TACGGGT	55	1.8189894E-12	45.0	4
TACGCGG	20	7.0298585E-4	45.0	2
TCGTAAG	20	7.0298585E-4	45.0	1
CTATTAC	20	7.0298585E-4	45.0	13
ACGTATT	20	7.0298585E-4	45.0	21
CGGTCTA	45	3.8380676E-10	45.0	31
ATAACGG	50	2.1827873E-11	45.0	2
CGCACAG	25	3.887964E-5	45.0	43
GATTCGT	25	3.887964E-5	45.0	9
GCGTATG	45	3.8380676E-10	45.0	1
ACGACTA	20	7.0298585E-4	45.0	12
GTCGGAT	20	7.0298585E-4	45.0	19
TACGGGC	30	2.1632895E-6	44.999996	4
CTCGAGG	60	0.0	44.999996	2
AAGGGTA	205	0.0	42.80488	5
>>END_MODULE