FastQCFastQC Report
Thu 26 May 2016
SRR1545528_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545528_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1271497
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC54160.4259546031174277No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46520.36586794935418643No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTAT351.2120654E-745.00000440
ATACGTA351.2120654E-745.00000444
GACTACG351.2120654E-745.0000041
ACTATCG207.033569E-445.09
CCTTACG207.033569E-445.032
TACCCGT207.033569E-445.017
CTACTAT253.8910406E-545.039
CGTGCTA207.033569E-445.025
AACCCGC253.8910406E-545.033
CGGTTAC253.8910406E-545.038
GGCGTAG551.8189894E-1245.01
ACTTACG207.033569E-445.025
TTATCGT253.8910406E-545.031
ATCGTCC253.8910406E-545.021
ATATCGT406.8157533E-945.037
GTAGCGT253.8910406E-545.035
ATATCGA406.8157533E-945.036
TACGCAT406.8157533E-945.040
CGTAGAT207.033569E-445.039
GTCGTTG253.8910406E-545.011