##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545528_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1271497
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.37057735881406	31.0	31.0	33.0	30.0	34.0
2	31.72543309185944	31.0	31.0	34.0	30.0	34.0
3	31.82643922872016	31.0	31.0	34.0	30.0	34.0
4	35.533417695834125	37.0	35.0	37.0	33.0	37.0
5	35.39340242249883	37.0	35.0	37.0	33.0	37.0
6	35.474364469597646	37.0	35.0	37.0	33.0	37.0
7	35.88039138118297	37.0	35.0	37.0	35.0	37.0
8	35.8945903922699	37.0	35.0	37.0	35.0	37.0
9	37.62493737696589	39.0	37.0	39.0	35.0	39.0
10	37.018121946021104	39.0	37.0	39.0	33.0	39.0
11	36.60622242915241	39.0	35.0	39.0	32.0	39.0
12	35.46949147343643	37.0	35.0	39.0	31.0	39.0
13	34.98227758303795	37.0	34.0	39.0	30.0	39.0
14	35.88798793862667	37.0	35.0	40.0	30.0	41.0
15	36.22000209202224	37.0	35.0	40.0	31.0	41.0
16	36.4570156280353	37.0	35.0	40.0	32.0	41.0
17	36.42744811824173	37.0	35.0	40.0	32.0	41.0
18	36.38446178009071	37.0	35.0	40.0	31.0	41.0
19	36.31071563676517	37.0	35.0	40.0	31.0	41.0
20	36.12073249091425	36.0	35.0	40.0	31.0	41.0
21	35.909525543512885	36.0	34.0	40.0	31.0	41.0
22	35.814634246089454	36.0	34.0	40.0	30.0	41.0
23	35.78950874441701	35.0	34.0	40.0	30.0	41.0
24	35.732371370125136	35.0	34.0	40.0	30.0	41.0
25	35.64186781408057	35.0	34.0	40.0	30.0	41.0
26	35.52300241369032	35.0	34.0	40.0	30.0	41.0
27	35.45215442899197	35.0	34.0	40.0	30.0	41.0
28	35.4653711333963	36.0	34.0	40.0	30.0	41.0
29	35.491401080773294	36.0	34.0	40.0	30.0	41.0
30	35.41120427338798	36.0	34.0	40.0	30.0	41.0
31	35.18252972677089	35.0	34.0	40.0	29.0	41.0
32	34.99369640667654	35.0	34.0	40.0	29.0	41.0
33	34.82598464644431	35.0	34.0	40.0	27.0	41.0
34	34.682728311588626	35.0	34.0	40.0	27.0	41.0
35	34.52745700540387	35.0	34.0	40.0	26.0	41.0
36	34.306239810239425	35.0	33.0	40.0	24.0	41.0
37	34.23793528415718	35.0	33.0	40.0	24.0	41.0
38	34.26874935607398	35.0	33.0	40.0	25.0	41.0
39	34.24417281362048	35.0	33.0	40.0	25.0	41.0
40	34.009970924036786	35.0	33.0	40.0	23.0	41.0
41	34.00157294905139	35.0	33.0	40.0	23.0	41.0
42	33.96426888934854	35.0	33.0	39.0	24.0	41.0
43	33.88068709560463	35.0	33.0	39.0	23.0	41.0
44	33.850617815063664	35.0	33.0	39.0	23.0	41.0
45	33.81301725446462	35.0	33.0	39.0	23.0	41.0
46	33.81588395411078	35.0	33.0	39.0	23.0	41.0
47	33.75372179407423	35.0	33.0	39.0	23.0	41.0
48	33.73289280273567	35.0	33.0	39.0	23.0	41.0
49	33.70304530801095	35.0	33.0	39.0	24.0	41.0
50	33.53442910207417	35.0	33.0	39.0	24.0	41.0
51	33.3473700685098	35.0	33.0	38.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	1.0
13	5.0
14	11.0
15	59.0
16	146.0
17	366.0
18	699.0
19	1519.0
20	2529.0
21	4048.0
22	5943.0
23	8666.0
24	12117.0
25	16792.0
26	21752.0
27	25657.0
28	28305.0
29	31825.0
30	37893.0
31	45999.0
32	57800.0
33	76418.0
34	138583.0
35	226952.0
36	79176.0
37	95280.0
38	131928.0
39	220888.0
40	138.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.00684390132261	23.83135784040387	27.338169103033668	14.823629155239848
2	31.203140864665823	25.95303016837633	29.93384962764364	12.909979339314209
3	28.464400623831594	25.163331097124097	31.72473077010799	14.647537508936317
4	26.473361714577383	27.992515908413466	30.149107705326873	15.385014671682276
5	23.19698748797677	33.31537549832992	29.238291557117318	14.249345456575988
6	21.09741509417639	41.004186403900285	27.0153213102351	10.883077191688223
7	87.04975316497011	4.504218256118575	6.746063891617518	1.6999646872937961
8	88.43929635697135	3.54141614176046	6.45380995786856	1.5654775433996304
9	85.19343734196777	4.7077578633689265	7.56871624549645	2.530088549166848
10	57.49639991285862	19.039368555332807	12.992165927249532	10.472065604559036
11	50.90047400819664	19.59745087876731	17.437005356677997	12.065069756358056
12	45.96959332188751	19.965520956793448	22.042914768969176	12.021970952349868
13	21.82498267789857	43.453504019278064	21.63190318183999	13.089610120983378
14	14.975811975962191	46.00577115006956	25.52463749422924	13.493779379739001
15	13.266016357097184	21.98070463398655	51.30236249082773	13.45091651808852
16	14.463817059733527	16.60963415564488	50.24361048433461	18.682938300286985
17	15.227090586922346	16.30338097533852	28.677299277937742	39.7922291598014
18	21.726673362186464	21.811691258414296	36.64090438278659	19.820730996612653
19	31.52237087464618	23.37811257124476	24.371823134462765	20.72769341964629
20	33.70027613120597	21.126593299079747	25.040405128757676	20.132725440956605
21	22.231039475515868	27.525035450339246	28.230267157531635	22.01365791661325
22	22.601390329666526	23.506150624028212	24.920782353399183	28.97167669290608
23	19.17008062150363	31.002747155518257	24.378193578120907	25.448978644857206
24	19.470749832677544	21.44283470586246	42.11657597304595	16.969839488414053
25	16.686944601520885	23.515195081073724	38.18978731369402	21.60807300371137
26	16.259417049352063	35.86292378196724	26.735415026539584	21.142244142141113
27	17.504720813340498	36.29383317459656	27.45102819747117	18.750417814591778
28	14.848167160441589	29.220202643026287	39.01519232841289	16.916437868119232
29	15.653517074755191	23.955542168011405	38.6215618283016	21.7693789289318
30	18.282544119254705	31.814074276227156	30.92425699785371	18.979124606664428
31	30.481078602623523	26.719056356405087	24.139577207024477	18.660287833946914
32	31.679272542522714	25.423654165129765	26.63018473500134	16.266888557346185
33	30.04450659340919	27.686498670464815	23.845199792056135	18.42379494406986
34	19.831112460351854	27.305845000027528	27.378515246201918	25.4845272934187
35	20.981646043993813	24.922984482071133	31.18410818114396	22.911261292791096
36	32.95548475537103	24.238201112546864	24.635449395476357	18.17086473660575
37	21.144839508075915	33.8276063569163	27.955787548063427	17.071766586944364
38	20.936030521503394	34.52143418348608	21.9958835923325	22.546651702678027
39	22.060846388155063	32.01667011404667	26.039935603465835	19.88254789433243
40	25.48051627333765	25.571904613223623	24.51873657586294	24.428842537575786
41	16.801927177177767	24.28924330926459	26.916146872544726	31.992682641012916
42	22.55498833265041	25.250000589855894	23.296869752740275	28.898141324753418
43	22.526203365009906	25.2614044704785	26.366715768892888	25.845676395618707
44	19.493872183733032	30.22004770754473	28.402347783754113	21.88373232496813
45	16.83700394102385	39.69887463360118	21.897416981715253	21.566704443659717
46	22.117000669289823	34.35729694997314	24.980003885184157	18.54569849555288
47	22.071070556989124	27.889566392999747	26.035767288479644	24.003595761531486
48	23.90921881844786	24.01271886602957	30.82311637384909	21.254945941673476
49	22.08467656628368	23.311970063633655	32.468735671417235	22.134617698665433
50	19.21852745228656	35.121514246592795	25.156252826392826	20.50370547472782
51	18.38517904485815	35.63484616951514	23.43308714059097	22.546887645035735
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1063.0
1	1762.0
2	2461.0
3	5522.0
4	8583.0
5	6521.0
6	4459.0
7	4780.0
8	5101.0
9	5405.0
10	5709.0
11	5984.5
12	6260.0
13	6102.0
14	5944.0
15	5739.5
16	5535.0
17	5178.5
18	4822.0
19	4857.5
20	4893.0
21	5371.0
22	5849.0
23	5503.5
24	5158.0
25	5335.5
26	6678.5
27	7844.0
28	8383.0
29	8922.0
30	12748.0
31	16574.0
32	19127.0
33	21680.0
34	24208.5
35	26737.0
36	30691.0
37	34645.0
38	41781.0
39	48917.0
40	69419.5
41	89922.0
42	114129.0
43	138336.0
44	138083.5
45	137831.0
46	132561.0
47	127291.0
48	112301.0
49	97311.0
50	93816.0
51	90321.0
52	80463.0
53	70605.0
54	63014.5
55	55424.0
56	49723.5
57	44023.0
58	43778.5
59	43534.0
60	41518.0
61	39502.0
62	35787.0
63	32072.0
64	26916.0
65	21760.0
66	18265.5
67	14771.0
68	12926.5
69	11082.0
70	9592.0
71	8102.0
72	6719.5
73	5337.0
74	4186.5
75	2562.5
76	2089.0
77	1668.5
78	1248.0
79	1000.5
80	753.0
81	519.0
82	285.0
83	209.5
84	134.0
85	88.5
86	43.0
87	27.0
88	11.0
89	6.5
90	2.0
91	2.5
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1271497.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.160159786105739
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.39522407862364	12.339646028578159
2	6.914627901244484	2.096537276886627
3	2.339408729317209	1.0639743042437844
4	1.0903699078510258	0.6612072811593177
5	0.5926933104214333	0.44926626450724494
6	0.42532594217229264	0.38688055467047555
7	0.31742421509156044	0.33685412745370774
8	0.25882189072730766	0.31390249756543875
9	0.2080045004327037	0.283804331651
>10	2.9490955535921786	11.199736993766981
>50	1.5316377455661856	17.051195203448025
>100	1.9664185079222678	52.11755017963981
>500	0.00990507731986296	0.9037414815035479
>1k	5.213198589401558E-4	0.3676611606431574
>5k	5.213198589401558E-4	0.4280423142827473
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5416	0.4259546031174277	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4652	0.36586794935418643	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.024773947559451576	0.0
2	0.0	0.0	0.0	0.0701535276921613	0.0
3	0.0	0.0	0.0	0.09744419373384286	0.0
4	0.0	0.0	0.0	0.15320523760575133	0.0
5	0.0	0.0	0.0	0.24671705871110983	0.0
6	0.0	0.0	0.0	0.35454271618415145	0.0
7	0.0	0.0	0.0	0.41077564477147804	0.0
8	0.0	0.0	0.0	0.5488019240312797	0.0
9	0.0	0.0	0.0	0.5973274022667768	0.0
10	0.0	0.0	0.0	0.6844687797139907	0.0
11	0.0	0.0	0.0	0.8093609343946545	0.0
12	0.0	0.0	0.0	0.9132542192392117	0.0
13	0.0	0.0	0.0	0.9565889656051095	0.0
14	0.0	0.0	0.0	0.9706668596150836	0.0
15	0.0	0.0	0.0	0.9970924036784986	0.0
16	7.864745256968754E-5	0.0	0.0	1.0532466848132556	0.0
17	7.864745256968754E-5	0.0	0.0	1.1230069752425684	0.0
18	7.864745256968754E-5	0.0	0.0	1.2194287520930054	0.0
19	7.864745256968754E-5	0.0	0.0	1.2641791526051576	0.0
20	1.572949051393751E-4	0.0	0.0	1.3060195973722313	0.0
21	1.572949051393751E-4	0.0	0.0	1.368780264522842	0.0
22	1.572949051393751E-4	0.0	0.0	1.4305971622426163	0.0
23	1.572949051393751E-4	0.0	0.0	1.509244614812304	0.0
24	1.572949051393751E-4	0.0	0.0	1.5664999602830365	0.0
25	1.572949051393751E-4	0.0	0.0	1.6100706490066434	0.0
26	1.572949051393751E-4	0.0	0.0	1.655764818949632	0.0
27	1.572949051393751E-4	0.0	0.0	1.7005938669143537	0.0
28	2.3594235770906262E-4	0.0	0.0	1.7525798330629172	0.0
29	2.3594235770906262E-4	0.0	0.0	1.8020490807292506	0.0
30	2.3594235770906262E-4	0.0	0.0	1.8746406794510722	0.0
31	2.3594235770906262E-4	0.0	0.0	1.9431426106392702	0.0
32	2.3594235770906262E-4	0.0	0.0	2.0029933220448024	0.0
33	2.3594235770906262E-4	0.0	0.0	2.0635518605234617	0.0
34	2.3594235770906262E-4	0.0	0.0	2.13449186274132	0.0
35	2.3594235770906262E-4	0.0	0.0	2.231621466664884	0.0
36	2.3594235770906262E-4	0.0	0.0	2.3057073669855295	0.0
37	2.3594235770906262E-4	0.0	0.0	2.383411050124381	0.0
38	2.3594235770906262E-4	0.0	0.0	2.461429323073511	0.0
39	2.3594235770906262E-4	0.0	0.0	2.535200633583878	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCTAT	35	1.2120654E-7	45.000004	40
ATACGTA	35	1.2120654E-7	45.000004	44
GACTACG	35	1.2120654E-7	45.000004	1
ACTATCG	20	7.033569E-4	45.0	9
CCTTACG	20	7.033569E-4	45.0	32
TACCCGT	20	7.033569E-4	45.0	17
CTACTAT	25	3.8910406E-5	45.0	39
CGTGCTA	20	7.033569E-4	45.0	25
AACCCGC	25	3.8910406E-5	45.0	33
CGGTTAC	25	3.8910406E-5	45.0	38
GGCGTAG	55	1.8189894E-12	45.0	1
ACTTACG	20	7.033569E-4	45.0	25
TTATCGT	25	3.8910406E-5	45.0	31
ATCGTCC	25	3.8910406E-5	45.0	21
ATATCGT	40	6.8157533E-9	45.0	37
GTAGCGT	25	3.8910406E-5	45.0	35
ATATCGA	40	6.8157533E-9	45.0	36
TACGCAT	40	6.8157533E-9	45.0	40
CGTAGAT	20	7.033569E-4	45.0	39
GTCGTTG	25	3.8910406E-5	45.0	11
>>END_MODULE