FastQCFastQC Report
Thu 26 May 2016
SRR1545527_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545527_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences992085
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62120.6261560249373793No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC25620.2582440012700525No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT15550.1567406018637516No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG10190.10271297318274139No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATACG351.2117016E-745.0000041
CTTACCG551.8189894E-1245.0000041
TTTAGCG502.1827873E-1145.01
CTATGCG207.0328184E-445.01
GTACCTA207.0328184E-445.022
CCTTAGT406.8139343E-945.043
GATACCC406.8139343E-945.03
TACTTCG253.890419E-545.029
GATCGAC253.890419E-545.09
GCGCAAA253.890419E-545.010
ATCCGTT253.890419E-545.033
ATATCGT207.0328184E-445.01
TACGCGG406.8139343E-945.02
GCCGACC253.890419E-545.014
TACGCAT253.890419E-545.038
CGTAGGC453.8380676E-1045.039
TATTACG207.0328184E-445.01
TCGTAAT253.890419E-545.033
CGTAATC253.890419E-545.034
ACGTATA207.0328184E-445.036