FastQCFastQC Report
Thu 26 May 2016
SRR1545526_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545526_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1734560
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85360.49211327368323954No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC82750.47706623005257814No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17640.10169726040033207No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTAA207.0342806E-445.00000415
CCTTACG207.0342806E-445.00000423
ACCGGAT406.8193913E-945.00000421
CGAACCG207.0342806E-445.00000429
TCACCGA207.0342806E-445.00000444
CGAAACA302.1661435E-645.00000444
ACGTTAC207.0342806E-445.00000437
GCGCGAA207.0342806E-445.00000420
CACATCG207.0342806E-445.00000419
CATTGCG207.0342806E-445.0000041
CGACATA302.1661435E-645.00000441
TCAATCG406.8193913E-945.0000041
TCGGAAT302.1661435E-645.0000041
TACGCCC406.8193913E-945.00000410
ATTCACG207.0342806E-445.00000410
ACGTGCC302.1661435E-645.00000419
TCGTCCA302.1661435E-645.00000433
TTGCGAT302.1661435E-645.0000041
CGTACTC207.0342806E-445.00000431
ACGTCGT406.8193913E-945.00000438