##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545526_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1734560
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.343694078037082	31.0	31.0	33.0	30.0	34.0
2	31.682316552901025	31.0	31.0	34.0	30.0	34.0
3	31.758505326999355	31.0	31.0	34.0	30.0	34.0
4	35.46017952679642	37.0	35.0	37.0	33.0	37.0
5	35.32757702241491	37.0	35.0	37.0	33.0	37.0
6	35.40408345632322	37.0	35.0	37.0	33.0	37.0
7	35.879819089567384	37.0	35.0	37.0	35.0	37.0
8	35.919052670417855	37.0	35.0	37.0	35.0	37.0
9	37.64794875933954	39.0	37.0	39.0	35.0	39.0
10	37.01403064754174	39.0	37.0	39.0	32.0	39.0
11	36.53830942717462	39.0	35.0	39.0	32.0	39.0
12	35.31922101282169	35.0	35.0	39.0	31.0	39.0
13	34.74557121114288	35.0	33.0	39.0	27.0	39.0
14	35.62892664422101	37.0	34.0	40.0	27.0	41.0
15	36.04260273498755	37.0	35.0	40.0	30.0	41.0
16	36.31646815330689	37.0	35.0	40.0	32.0	41.0
17	36.25993796697722	36.0	35.0	40.0	31.0	41.0
18	36.22315169264828	36.0	35.0	40.0	31.0	41.0
19	36.13193086431141	36.0	35.0	40.0	31.0	41.0
20	35.93256906650678	36.0	34.0	40.0	31.0	41.0
21	35.70255915044738	35.0	34.0	40.0	30.0	41.0
22	35.61879669772161	35.0	34.0	40.0	30.0	41.0
23	35.64555103311503	35.0	34.0	40.0	30.0	41.0
24	35.57607174153676	35.0	34.0	40.0	30.0	41.0
25	35.47993958121945	35.0	34.0	40.0	30.0	41.0
26	35.33635850013837	35.0	34.0	40.0	30.0	41.0
27	35.2443276681118	35.0	34.0	40.0	29.0	41.0
28	35.288270800664144	36.0	34.0	40.0	29.0	41.0
29	35.31183873720136	36.0	34.0	40.0	30.0	41.0
30	35.23672977585094	36.0	34.0	40.0	29.0	41.0
31	34.99702921778434	35.0	34.0	40.0	29.0	41.0
32	34.75111267410755	35.0	34.0	40.0	28.0	41.0
33	34.521657365556685	35.0	33.0	40.0	27.0	41.0
34	34.377219583064296	35.0	34.0	40.0	26.0	41.0
35	34.20569308643114	35.0	33.0	40.0	25.0	41.0
36	33.97825558066599	35.0	33.0	40.0	23.0	41.0
37	33.87915436767826	35.0	33.0	40.0	23.0	41.0
38	33.90749584909141	35.0	33.0	40.0	24.0	41.0
39	33.8982139562771	35.0	33.0	39.0	23.0	41.0
40	33.68499619500046	35.0	33.0	39.0	23.0	41.0
41	33.739487247486394	35.0	33.0	39.0	23.0	41.0
42	33.66092323125173	35.0	33.0	39.0	23.0	41.0
43	33.516796766903425	35.0	33.0	39.0	23.0	41.0
44	33.45473895397104	35.0	33.0	39.0	23.0	41.0
45	33.42401934784614	35.0	33.0	39.0	23.0	41.0
46	33.41589106171018	35.0	33.0	39.0	23.0	41.0
47	33.3606793653722	35.0	33.0	39.0	23.0	41.0
48	33.373206461581034	35.0	33.0	39.0	23.0	41.0
49	33.37152015496726	35.0	33.0	39.0	23.0	41.0
50	33.188290978692	35.0	32.0	38.0	23.0	40.0
51	32.99914675767918	35.0	32.0	38.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	1.0
12	2.0
13	6.0
14	24.0
15	51.0
16	190.0
17	501.0
18	1207.0
19	2318.0
20	3895.0
21	6130.0
22	9291.0
23	12921.0
24	18260.0
25	25559.0
26	32568.0
27	37874.0
28	41213.0
29	46366.0
30	54423.0
31	65708.0
32	82143.0
33	106794.0
34	193371.0
35	314642.0
36	106661.0
37	124489.0
38	170803.0
39	276997.0
40	150.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.653814223780095	24.017330043353937	27.291243888940137	16.037611843925838
2	29.511461119822897	26.43338944746795	30.751660363435107	13.303489069274052
3	26.783103495987454	26.745802970205702	32.89450927036251	13.576584263444333
4	23.70307167235495	28.73431878977954	31.966377640439074	15.596231897426438
5	22.11875057651508	33.13860575592658	31.291047873812378	13.451595793745966
6	20.312240568213262	41.40329536020662	27.061329674384282	11.22313439719583
7	86.65817267779725	4.555391569043446	7.233477077760354	1.5529586753989486
8	88.22352642745133	3.3493796697721607	6.757217968822064	1.6698759339544325
9	85.36251268333179	4.0759616271561665	8.128113181440828	2.433412508071211
10	58.83492067152477	18.123558712295914	13.19395120376349	9.847569412415828
11	51.52972511760908	18.50613412046859	18.2679180887372	11.69622267318513
12	45.90489807213356	19.48805460750853	22.850521169633797	11.756526150724103
13	19.41622082833687	45.41987593395444	23.29680149432709	11.867101743381607
14	13.168872797712389	47.880038741813486	27.353046305691358	11.59804215478277
15	12.09903376072318	21.43344709897611	53.6053523660179	12.862166774282816
16	13.701803339175353	16.351927866432987	51.70417858131169	18.242090213079972
17	13.935003689696524	15.7051355963472	29.10057882114196	41.259281892814315
18	20.14983626971682	20.733442486855456	38.49777465178489	20.618946591642835
19	29.79401116133198	22.407872889954803	26.93259385665529	20.86552209205793
20	33.38431648371921	21.100163730283185	25.573920763767184	19.94159902223042
21	20.61295083479384	28.574508809150444	30.0310741629001	20.781466193155612
22	21.507990499031454	22.68932755280878	26.01184161977677	29.790840328382988
23	17.524271284936813	31.487178304584447	24.326860990683517	26.66168941979522
24	18.097557882114195	21.841677428281525	43.726074624112165	16.334690065492115
25	15.83266073240476	22.940457522368785	39.86382713771792	21.363054607508534
26	15.62920856009593	36.02100820957476	28.606620699197492	19.743162531131812
27	15.791382252559726	37.4275897057467	28.206461581034958	18.574566460658612
28	13.66484872244258	29.6076814869477	40.029978784244996	16.697491006364725
29	13.880061802416751	23.246471727700396	40.244961258186514	22.628505211696336
30	16.47951065399871	33.059738492758974	32.29591366110137	18.164837192140944
31	30.938047689327554	26.645028133935984	24.61108292592934	17.805841250807124
32	31.518137164468225	25.366721243427726	27.248293515358363	15.866848076745688
33	30.23936906189466	27.9700903975648	23.739853334563232	18.050687205977308
34	19.552393690618945	28.55727100820957	26.445784521723088	25.444550779448388
35	19.176967069458538	26.080792823540268	32.90661608707684	21.83562401992436
36	34.96961765519786	22.657215662761736	25.2039710358823	17.1691956461581
37	20.56786735541002	33.92560649386588	28.41262337422747	17.093902776496634
38	20.51211834701596	34.484019001937085	22.693247855363897	22.310614795683055
39	20.006111059865326	33.00531546905267	26.774513421271102	20.214060049810904
40	25.58106955077945	25.287335116686656	24.418469237155243	24.713126095378655
41	16.460370353288443	24.42354256987363	27.85011760907665	31.26596946776128
42	22.690538234480215	24.130211696337977	24.445853703532887	28.73339636564893
43	22.8953163914768	23.811110598653258	26.72026335208929	26.573309657780648
44	18.60863850198321	29.811479568305504	30.52128493681395	21.058596992897336
45	16.2870699197491	40.94219859791532	21.860933032008116	20.90979845032746
46	22.4175006918181	33.47926851766442	26.2159279586754	17.88730283184208
47	21.435349598745503	28.119177197675494	26.126222211973065	24.31925099160594
48	23.492528364542018	24.40976385942256	31.939915598192048	20.157792177843373
49	22.658829904990316	22.421305691356885	33.89251452818006	21.02734987547274
50	20.249515727331428	35.24375057651508	25.26306383174984	19.243669864403653
51	18.673265842634443	35.4353841896504	22.96294161055253	22.928408357162624
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2425.0
1	2806.0
2	3187.0
3	9077.5
4	14968.0
5	11173.0
6	7378.0
7	8123.5
8	8869.0
9	9600.0
10	10331.0
11	10681.0
12	11031.0
13	11020.0
14	11009.0
15	10339.5
16	9670.0
17	8895.0
18	8120.0
19	7951.5
20	7783.0
21	7707.0
22	7631.0
23	7442.0
24	7253.0
25	8643.5
26	10223.5
27	10413.0
28	12190.5
29	13968.0
30	17060.5
31	20153.0
32	21884.5
33	23616.0
34	30320.5
35	37025.0
36	41351.5
37	45678.0
38	59177.5
39	72677.0
40	103985.0
41	135293.0
42	162408.5
43	189524.0
44	194942.0
45	200360.0
46	187869.0
47	175378.0
48	156392.5
49	137407.0
50	126693.0
51	115979.0
52	105180.0
53	94381.0
54	85665.5
55	76950.0
56	70476.0
57	64002.0
58	57272.5
59	50543.0
60	47869.5
61	45196.0
62	39538.0
63	33880.0
64	28577.5
65	23275.0
66	20487.5
67	17700.0
68	14497.0
69	11294.0
70	9430.5
71	7567.0
72	6793.0
73	6019.0
74	4152.0
75	1993.0
76	1701.0
77	1595.0
78	1489.0
79	1168.0
80	847.0
81	489.5
82	132.0
83	121.5
84	111.0
85	63.5
86	16.0
87	12.0
88	8.0
89	6.0
90	4.0
91	2.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1734560.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.78430151095141
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.3652978332641	12.84294393528602
2	7.857012502779195	2.4803490863836357
3	2.68326884277067	1.270605733477018
4	1.2800123059295683	0.8081640069808194
5	0.7302670365082988	0.5763377543877976
6	0.4821363621756438	0.4566111425984179
7	0.3335237256985721	0.3685107333237481
8	0.24163888225042715	0.30512807793680213
9	0.1983915578770117	0.2818324950082308
>10	2.0599379304498244	7.635130906479329
>50	0.9625454313068799	11.279344285934112
>100	1.7780250937204762	57.761199330680526
>500	0.0246335155664334	2.461074458275897
>1k	0.002573650880075131	0.4971709319951333
>5k	7.353288228786088E-4	0.9755971212525021
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8536	0.49211327368323954	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	8275	0.47706623005257814	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1764	0.10169726040033207	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.765150816345356E-5	0.0	0.0	0.05032976662669495	0.0
2	5.765150816345356E-5	0.0	0.0	0.17900793284752328	0.0
3	5.765150816345356E-5	0.0	0.0	0.24265519785997602	0.0
4	5.765150816345356E-5	0.0	0.0	0.3693732128032469	0.0
5	5.765150816345356E-5	0.0	0.0	0.5801471266488332	0.0
6	5.765150816345356E-5	0.0	0.0	0.7793907388617286	0.0
7	1.1530301632690712E-4	0.0	0.0	0.8881791347661655	0.0
8	1.1530301632690712E-4	0.0	0.0	1.1031616087076839	0.0
9	1.1530301632690712E-4	0.0	0.0	1.18606447744673	0.0
10	1.1530301632690712E-4	0.0	0.0	1.3441449128309197	0.0
11	1.1530301632690712E-4	0.0	0.0	1.5413707222580944	0.0
12	1.1530301632690712E-4	0.0	0.0	1.7072917627525137	0.0
13	1.1530301632690712E-4	0.0	0.0	1.7733027395996679	0.0
14	1.1530301632690712E-4	0.0	0.0	1.8020131906650678	0.0
15	1.1530301632690712E-4	0.0	0.0	1.8476155336223596	0.0
16	1.1530301632690712E-4	0.0	0.0	1.9508117332349415	0.0
17	1.1530301632690712E-4	0.0	0.0	2.077068536112905	0.0
18	1.7295452449036066E-4	0.0	0.0	2.24910063647265	0.0
19	1.7295452449036066E-4	0.0	0.0	2.328313808689235	0.0
20	1.7295452449036066E-4	0.0	0.0	2.4044714509731575	0.0
21	1.7295452449036066E-4	0.0	0.0	2.5025943178673553	0.0
22	1.7295452449036066E-4	0.0	0.0	2.598757033483996	0.0
23	1.7295452449036066E-4	0.0	0.0	2.723976109215017	0.0
24	1.7295452449036066E-4	0.0	0.0	2.8131053408357163	0.0
25	1.7295452449036066E-4	0.0	0.0	2.879923438797159	0.0
26	1.7295452449036066E-4	0.0	0.0	2.952506687574947	0.0
27	1.7295452449036066E-4	0.0	0.0	3.021515542846601	0.0
28	1.7295452449036066E-4	0.0	0.0	3.09461765519786	0.0
29	1.7295452449036066E-4	0.0	0.0	3.17123650954709	0.0
30	1.7295452449036066E-4	0.0	0.0	3.2792754358454017	0.0
31	1.7295452449036066E-4	0.0	0.0	3.3794161055253205	0.0
32	2.3060603265381423E-4	0.0	0.0	3.467161700950097	0.0
33	2.3060603265381423E-4	0.0	0.0	3.5507563877871045	0.0
34	2.3060603265381423E-4	0.0	0.0	3.640462134489438	0.0
35	2.882575408172678E-4	0.0	0.0	3.771619315561295	0.0
36	2.882575408172678E-4	0.0	0.0	3.876602711926944	0.0
37	2.882575408172678E-4	0.0	0.0	3.9744373212803246	0.0
38	2.882575408172678E-4	0.0	0.0	4.0779794299418874	0.0
39	2.882575408172678E-4	0.0	0.0	4.1812332810626325	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGTAA	20	7.0342806E-4	45.000004	15
CCTTACG	20	7.0342806E-4	45.000004	23
ACCGGAT	40	6.8193913E-9	45.000004	21
CGAACCG	20	7.0342806E-4	45.000004	29
TCACCGA	20	7.0342806E-4	45.000004	44
CGAAACA	30	2.1661435E-6	45.000004	44
ACGTTAC	20	7.0342806E-4	45.000004	37
GCGCGAA	20	7.0342806E-4	45.000004	20
CACATCG	20	7.0342806E-4	45.000004	19
CATTGCG	20	7.0342806E-4	45.000004	1
CGACATA	30	2.1661435E-6	45.000004	41
TCAATCG	40	6.8193913E-9	45.000004	1
TCGGAAT	30	2.1661435E-6	45.000004	1
TACGCCC	40	6.8193913E-9	45.000004	10
ATTCACG	20	7.0342806E-4	45.000004	10
ACGTGCC	30	2.1661435E-6	45.000004	19
TCGTCCA	30	2.1661435E-6	45.000004	33
TTGCGAT	30	2.1661435E-6	45.000004	1
CGTACTC	20	7.0342806E-4	45.000004	31
ACGTCGT	40	6.8193913E-9	45.000004	38
>>END_MODULE