FastQCFastQC Report
Thu 26 May 2016
SRR1545525_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545525_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1386756
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68380.49309323341669337No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC35330.25476724095659226No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAG551.8189894E-1245.01
GTCGACG351.2121745E-745.01
GCGAATC207.0337916E-445.017
ACGTTAG406.8175723E-945.01
GACGTAT207.0337916E-445.029
GACCGAA253.8912243E-545.023
ATCGTAC253.8912243E-545.028
CCGTAAC351.2121745E-745.018
CTACGTT207.0337916E-445.030
CATCGTA207.0337916E-445.027
CGTCGTT207.0337916E-445.012
TTCGATC253.8912243E-545.020
AGTCGTA207.0337916E-445.023
GATCGAC302.165827E-644.9999969
CTATCGA302.165827E-644.99999627
CGACGGT1700.042.3529428
TACGGGT850.042.352944
CTAAGCG700.041.7857131
CGTACAT650.041.5384635
TCGTACA650.041.5384634
Produced by FastQC (version 0.11.5)