##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545525_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1386756
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.317199276585065	31.0	31.0	33.0	30.0	34.0
2	31.661583580673167	31.0	31.0	34.0	30.0	34.0
3	31.75786656051966	31.0	31.0	34.0	30.0	34.0
4	35.466111558197696	37.0	35.0	37.0	33.0	37.0
5	35.32277560003346	37.0	35.0	37.0	33.0	37.0
6	35.40685960615999	37.0	35.0	37.0	33.0	37.0
7	35.849058522191356	37.0	35.0	37.0	35.0	37.0
8	35.88284096120731	37.0	35.0	37.0	35.0	37.0
9	37.57916677483278	39.0	37.0	39.0	35.0	39.0
10	36.92654872234193	39.0	37.0	39.0	32.0	39.0
11	36.5188165762398	39.0	35.0	39.0	32.0	39.0
12	35.670058034722764	37.0	35.0	39.0	31.0	39.0
13	35.276823752700544	37.0	35.0	39.0	30.0	39.0
14	36.24753597604769	38.0	35.0	40.0	30.0	41.0
15	36.52584016222032	38.0	35.0	40.0	31.0	41.0
16	36.6906074320212	38.0	35.0	41.0	32.0	41.0
17	36.62525995921417	38.0	35.0	40.0	32.0	41.0
18	36.561181635413874	38.0	35.0	40.0	31.0	41.0
19	36.510345727727156	37.0	35.0	40.0	31.0	41.0
20	36.356396510994	37.0	35.0	40.0	31.0	41.0
21	36.15427010952179	37.0	35.0	40.0	31.0	41.0
22	36.07846802177168	37.0	35.0	40.0	30.0	41.0
23	36.061700111627424	37.0	34.0	40.0	30.0	41.0
24	35.98502620504256	37.0	34.0	40.0	30.0	41.0
25	35.86451185356328	37.0	34.0	40.0	30.0	41.0
26	35.76787841552515	37.0	34.0	40.0	30.0	41.0
27	35.72179893218418	37.0	34.0	40.0	30.0	41.0
28	35.69801825267026	36.0	34.0	40.0	30.0	41.0
29	35.67745226990184	36.0	34.0	40.0	30.0	41.0
30	35.59392928532489	36.0	34.0	40.0	30.0	41.0
31	35.41808220047362	36.0	34.0	40.0	29.0	41.0
32	35.2534944864129	36.0	34.0	40.0	29.0	41.0
33	35.08226753661062	36.0	34.0	40.0	27.0	41.0
34	34.9133394771683	36.0	34.0	40.0	27.0	41.0
35	34.76663378416967	36.0	34.0	40.0	26.0	41.0
36	34.59379371713553	36.0	33.0	40.0	25.0	41.0
37	34.52008284081698	36.0	33.0	40.0	25.0	41.0
38	34.50970826879422	35.0	33.0	40.0	25.0	41.0
39	34.45068130226226	36.0	33.0	40.0	25.0	41.0
40	34.2658448926848	35.0	33.0	40.0	24.0	41.0
41	34.23999751939058	35.0	33.0	40.0	24.0	41.0
42	34.1711735878554	35.0	33.0	40.0	24.0	41.0
43	34.08331242121902	35.0	33.0	40.0	23.0	41.0
44	34.00439731286542	35.0	33.0	40.0	23.0	41.0
45	33.96294733896951	35.0	33.0	40.0	23.0	41.0
46	33.93225268179838	35.0	33.0	40.0	23.0	41.0
47	33.88962513953428	35.0	33.0	40.0	23.0	41.0
48	33.85504082910043	35.0	33.0	39.0	23.0	41.0
49	33.79192229923649	35.0	33.0	39.0	24.0	41.0
50	33.65561713812668	35.0	33.0	39.0	24.0	41.0
51	33.47896746075013	35.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	3.0
13	4.0
14	22.0
15	52.0
16	146.0
17	329.0
18	826.0
19	1584.0
20	2763.0
21	4398.0
22	6439.0
23	9469.0
24	13162.0
25	17788.0
26	23309.0
27	27490.0
28	30502.0
29	35244.0
30	41609.0
31	49956.0
32	61903.0
33	79872.0
34	136619.0
35	205969.0
36	96251.0
37	117106.0
38	162104.0
39	261718.0
40	119.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.674593079099715	22.9620062938253	28.09052205290621	14.272878574168779
2	31.382593621372468	25.091436417076977	29.80841618857247	13.717553772978086
3	30.135871054460917	24.559042830894548	31.423047745962517	13.882038368682018
4	26.084040739683118	27.919619601429524	30.86692972664261	15.12940993224475
5	23.11509739276412	32.17400898211365	30.314922019446822	14.395971605675403
6	20.987397927248917	42.427939738497614	26.3790457730127	10.205616561240765
7	86.48053442710902	4.39738497616019	7.226577710859012	1.8955028858717755
8	87.43564116542491	3.3825705459359834	7.151221988583428	2.0305663000556695
9	83.62776148075076	4.652296438594821	8.562429151198913	3.157512929455506
10	51.36967137694014	22.390889240789296	14.973001739311027	11.26643764295954
11	43.91378151599849	21.310670370274224	20.46661417004866	14.308933943678628
12	40.21262572507348	20.837984476000106	24.64701793249858	14.302371866427835
13	22.06292959972771	37.59039081136119	24.32504348277563	16.02163610613547
14	16.275970682657945	39.71902771648365	27.527841956335504	16.477159644522903
15	15.693460132856826	22.705364173654196	45.87461673142211	15.726558962066866
16	17.630570915142968	18.012541499730304	44.550014566369285	19.806873018757447
17	17.41294070478152	18.974859311948173	29.127474480009464	34.48472550326085
18	21.134792277805182	23.39589661050682	34.06669954916366	21.40261156252434
19	28.870688138360318	23.760488506990416	26.34068285985422	21.028140494795046
20	31.5943107511343	21.63048149782658	26.63979820530793	20.13540954573119
21	22.549821309588708	26.94504296357831	28.837012423238118	21.668123303594864
22	22.508213413174342	24.311919328274044	26.16213667004145	27.017730588510165
23	19.869465140226545	28.44833553992195	26.15557459279066	25.526624727060852
24	19.53660196891162	22.84540322883045	39.17084187845591	18.447152923802022
25	18.562457995494523	24.104024067680253	35.66178909627937	21.67172884054585
26	17.94886771717591	32.79978597532659	27.411743666513793	21.839602640983706
27	18.990723674532507	33.17829524444098	27.77453279452189	20.05644828650462
28	16.73300854656479	28.202077366169682	36.23853078695892	18.826383300306617
29	17.113176362676636	24.06688703708511	35.65097248542642	23.168964114811835
30	19.330581587532343	30.191684766462163	31.55428929097837	18.923444355027126
31	28.12924551975978	27.227428617579445	25.265006965897392	19.378318896763382
32	29.10620181199865	25.439370732847017	27.023860001326838	18.430567453827493
33	28.24757924249111	27.66240059534626	24.16077521928876	19.929244942873872
34	20.49942455630262	28.097516794591115	26.878124197768027	24.524934451338233
35	21.062248874351365	25.49633821667258	30.33850223110627	23.102910677869794
36	29.295204058969276	26.619462976904373	25.386225118189504	18.69910784593685
37	21.281681853188303	32.627585530547556	27.82248643596999	18.268246180294152
38	21.5591639769361	32.65866525906504	24.196470035103506	21.58570072889535
39	21.7165817202161	30.486401356835664	26.503508908560697	21.293508014387534
40	24.292377317999705	25.90917219756035	26.85108267063564	22.947367813804302
41	18.166714259754418	25.636088828892756	28.339304102524167	27.85789280882866
42	22.14621750329546	25.86785274410206	24.707158288841008	27.27877146376147
43	22.297361612280746	25.630824744944313	26.741979122498837	25.329834520276094
44	19.21340163662533	29.944993928275775	28.293730115463717	22.547874319635177
45	18.1428456051389	36.75253613469132	23.640784680217717	21.46383357995206
46	21.683266558789	32.100816582008655	25.967149231732183	20.248767627470155
47	21.59110903432183	28.51229776543242	26.31234333941948	23.584249860826272
48	22.618182290179384	25.268396170631313	30.289106374877772	21.824315164311532
49	21.496788187684064	25.535854901655377	30.860944535303975	22.106412375356587
50	20.06596690405522	32.318951567543245	27.043041457906074	20.572040070495458
51	19.053387906740625	32.341954893290534	25.394229410220685	23.210427789748163
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1647.0
1	2580.0
2	3513.0
3	7751.5
4	11990.0
5	8854.0
6	5718.0
7	5883.0
8	6048.0
9	6396.5
10	6745.0
11	6880.5
12	7016.0
13	7006.5
14	6997.0
15	6508.0
16	6019.0
17	5928.5
18	5838.0
19	5659.5
20	5481.0
21	5221.5
22	4962.0
23	5841.5
24	6721.0
25	7012.5
26	9227.5
27	11151.0
28	13791.5
29	16432.0
30	17786.0
31	19140.0
32	23652.5
33	28165.0
34	32996.0
35	37827.0
36	40068.5
37	42310.0
38	45661.5
39	49013.0
40	71418.5
41	93824.0
42	110822.5
43	127821.0
44	127881.5
45	127942.0
46	126779.5
47	125617.0
48	115873.0
49	106129.0
50	100922.0
51	95715.0
52	86829.0
53	77943.0
54	70362.0
55	62781.0
56	59204.5
57	55628.0
58	53138.5
59	50649.0
60	46615.5
61	42582.0
62	40016.0
63	37450.0
64	32265.5
65	27081.0
66	23440.0
67	19799.0
68	17681.0
69	15563.0
70	14027.0
71	12491.0
72	9832.0
73	7173.0
74	6212.0
75	3919.5
76	2588.0
77	1932.5
78	1277.0
79	1020.5
80	764.0
81	568.0
82	372.0
83	270.5
84	169.0
85	109.0
86	49.0
87	36.0
88	23.0
89	19.0
90	15.0
91	12.5
92	10.0
93	10.5
94	11.0
95	6.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1386756.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.307388488614393
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.35077075946582	13.906620048936723
2	6.75190175702977	2.3371557349174465
3	2.285170977239748	1.1865102559798475
4	1.1928987854147868	0.8258385082707989
5	0.7530036365923051	0.6516263235921218
6	0.48576084107767886	0.5044350953452478
7	0.3943232005577062	0.47772933754882196
8	0.31347345461160686	0.4340325487864889
9	0.27420041137083684	0.42711237389996565
>10	3.9726139161055523	17.4894982394737
>50	1.8259315369732445	22.5379257042396
>100	1.3940809092063777	37.851631636713506
>500	0.0037734520850698943	0.39148878151475175
>1k	0.0016770898155866198	0.4821951720418027
>5k	4.1927245389665496E-4	0.49620023873917934
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6838	0.49309323341669337	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3533	0.25476724095659226	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.024445540527677542	0.0
2	0.0	0.0	0.0	0.08610022238951914	0.0
3	0.0	0.0	0.0	0.12504002146015594	0.0
4	0.0	0.0	0.0	0.18864169327552938	0.0
5	0.0	0.0	0.0	0.32247922489608843	0.0
6	0.0	0.0	0.0	0.462157726377243	0.0
7	0.0	0.0	0.0	0.5441476366426393	0.0
8	7.211073901969777E-5	0.0	0.0	0.7314913366158142	0.0
9	7.211073901969777E-5	0.0	0.0	0.7942276795629513	0.0
10	7.211073901969777E-5	0.0	0.0	0.9054224391313251	0.0
11	7.211073901969777E-5	0.0	0.0	1.0499323601267996	0.0
12	7.211073901969777E-5	0.0	0.0	1.165309542558316	0.0
13	7.211073901969777E-5	0.0	0.0	1.2149938417428878	0.0
14	7.211073901969777E-5	0.0	0.0	1.2350406271903638	0.0
15	7.211073901969777E-5	0.0	0.0	1.263380147625105	0.0
16	7.211073901969777E-5	0.0	0.0	1.332534346344995	0.0
17	7.211073901969777E-5	0.0	0.0	1.4183461257784353	0.0
18	7.211073901969777E-5	0.0	0.0	1.534372304861129	0.0
19	7.211073901969777E-5	0.0	0.0	1.590258127601395	0.0
20	7.211073901969777E-5	0.0	0.0	1.6491004906414684	0.0
21	7.211073901969777E-5	0.0	0.0	1.7301529612996085	0.0
22	7.211073901969777E-5	0.0	0.0	1.8135850863453988	0.0
23	7.211073901969777E-5	0.0	0.0	1.9178572149678819	0.0
24	7.211073901969777E-5	0.0	0.0	2.0012172292746526	0.0
25	7.211073901969777E-5	0.0	0.0	2.067631219911794	0.0
26	7.211073901969777E-5	0.0	0.0	2.138516076368157	0.0
27	7.211073901969777E-5	0.0	0.0	2.2002428689690183	0.0
28	7.211073901969777E-5	0.0	0.0	2.2694691784279284	0.0
29	1.4422147803939554E-4	0.0	0.0	2.3502332061299898	0.0
30	1.4422147803939554E-4	0.0	0.0	2.4480874789797196	0.0
31	1.4422147803939554E-4	0.0	0.0	2.540605557141992	0.0
32	1.4422147803939554E-4	0.0	0.0	2.6242540144048414	0.0
33	1.4422147803939554E-4	0.0	0.0	2.7059554817141587	0.0
34	1.4422147803939554E-4	0.0	0.0	2.79868989209349	0.0
35	1.4422147803939554E-4	0.0	0.0	2.9167351718687353	0.0
36	1.4422147803939554E-4	0.0	0.0	3.0153105521086623	0.0
37	1.4422147803939554E-4	0.0	0.0	3.114174375304668	0.0
38	1.4422147803939554E-4	0.0	0.0	3.2132545307177325	0.0
39	1.4422147803939554E-4	0.0	0.0	3.31406534386727	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGTAG	55	1.8189894E-12	45.0	1
GTCGACG	35	1.2121745E-7	45.0	1
GCGAATC	20	7.0337916E-4	45.0	17
ACGTTAG	40	6.8175723E-9	45.0	1
GACGTAT	20	7.0337916E-4	45.0	29
GACCGAA	25	3.8912243E-5	45.0	23
ATCGTAC	25	3.8912243E-5	45.0	28
CCGTAAC	35	1.2121745E-7	45.0	18
CTACGTT	20	7.0337916E-4	45.0	30
CATCGTA	20	7.0337916E-4	45.0	27
CGTCGTT	20	7.0337916E-4	45.0	12
TTCGATC	25	3.8912243E-5	45.0	20
AGTCGTA	20	7.0337916E-4	45.0	23
GATCGAC	30	2.165827E-6	44.999996	9
CTATCGA	30	2.165827E-6	44.999996	27
CGACGGT	170	0.0	42.35294	28
TACGGGT	85	0.0	42.35294	4
CTAAGCG	70	0.0	41.785713	1
CGTACAT	65	0.0	41.53846	35
TCGTACA	65	0.0	41.53846	34
>>END_MODULE