FastQCFastQC Report
Thu 26 May 2016
SRR1545524_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545524_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1715676
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71360.41592934796546666No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC39710.2314539575071284No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGCG700.045.0000041
ACGCCTA351.2123928E-745.00000436
ACCGTAG351.2123928E-745.00000445
TAGTCCG351.2123928E-745.0000041
AATCGTA351.2123928E-745.00000412
ATTTCGC351.2123928E-745.00000436
CGTATGG800.045.02
CCGATCA207.034261E-445.010
CCAATCG253.891613E-545.037
GAATCGT453.8562575E-1045.040
CTAACGC253.891613E-545.023
TCTCGAC207.034261E-445.013
GCGATCG207.034261E-445.09
TCCCGTA207.034261E-445.011
TCGTACT406.8175723E-945.018
TTACGTC453.8562575E-1045.032
ATCGGAT551.8189894E-1245.010
TACACGA406.8175723E-945.022
GTCGGTA207.034261E-445.025
TACGTCT453.8562575E-1045.033