FastQCFastQC Report
Thu 26 May 2016
SRR1545523_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545523_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1175229
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49900.4245980996044175No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC33830.2878587917759007No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT14500.12338020930388886No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG302.1655433E-645.0000041
TCGTTGA2400.045.00000424
GACGTAT302.1655433E-645.00000426
CCGGATA302.1655433E-645.00000419
ACGATAT302.1655433E-645.00000436
CCGACGG302.1655433E-645.0000042
ATACGTA453.8562575E-1045.00000444
TGGGACG600.045.0000046
GTACGTA453.8562575E-1045.00000423
TACGTAT453.8562575E-1045.00000445
CGAAGCA302.1655433E-645.00000440
CTATGCG253.8908605E-545.01
CCTTAGT253.8908605E-545.015
ACGTTAG502.1827873E-1145.01
CGTTGTC207.033352E-445.024
CACGCTA406.8157533E-945.016
CGCGACA207.033352E-445.043
ATCGTAC253.8908605E-545.041
CACCTCG207.033352E-445.025
CGTTAAT253.8908605E-545.022
Produced by FastQC (version 0.11.5)