##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545523_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1175229
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.292748051656314	31.0	31.0	33.0	30.0	34.0
2	31.62500670082171	31.0	31.0	34.0	30.0	34.0
3	31.717832014015993	31.0	31.0	34.0	30.0	34.0
4	35.42262146356157	37.0	35.0	37.0	33.0	37.0
5	35.27586538453357	37.0	35.0	37.0	33.0	37.0
6	35.3513825816075	37.0	35.0	37.0	33.0	37.0
7	35.81929734545353	37.0	35.0	37.0	35.0	37.0
8	35.84767564449142	37.0	35.0	37.0	35.0	37.0
9	37.52943043440895	39.0	37.0	39.0	35.0	39.0
10	36.901805520456016	39.0	37.0	39.0	32.0	39.0
11	36.47109031516411	39.0	35.0	39.0	32.0	39.0
12	35.40155578189442	37.0	35.0	39.0	31.0	39.0
13	34.862599544429216	37.0	34.0	39.0	28.0	39.0
14	35.764807539636955	37.0	34.0	40.0	29.0	41.0
15	36.121815407890715	37.0	35.0	40.0	30.0	41.0
16	36.35734482385986	37.0	35.0	40.0	31.0	41.0
17	36.32073238492243	37.0	35.0	40.0	31.0	41.0
18	36.26778780986514	37.0	35.0	40.0	31.0	41.0
19	36.19099086220643	37.0	35.0	40.0	31.0	41.0
20	36.004262148057954	36.0	34.0	40.0	31.0	41.0
21	35.77206825222999	36.0	34.0	40.0	30.0	41.0
22	35.70252095549038	36.0	34.0	40.0	30.0	41.0
23	35.6961009301166	35.0	34.0	40.0	30.0	41.0
24	35.626346014266154	35.0	34.0	40.0	30.0	41.0
25	35.52118948732545	35.0	34.0	40.0	30.0	41.0
26	35.392368636240256	35.0	34.0	40.0	30.0	41.0
27	35.32211679596062	35.0	34.0	40.0	29.0	41.0
28	35.327800794568546	36.0	34.0	40.0	29.0	41.0
29	35.342195435953336	36.0	34.0	40.0	29.0	41.0
30	35.27485536861327	36.0	34.0	40.0	29.0	41.0
31	35.07809116350941	35.0	34.0	40.0	29.0	41.0
32	34.86974964028287	35.0	34.0	40.0	28.0	41.0
33	34.683588475097196	35.0	34.0	40.0	27.0	41.0
34	34.537808376069684	35.0	34.0	40.0	26.0	41.0
35	34.37243635070271	35.0	33.0	40.0	25.0	41.0
36	34.16759627272642	35.0	33.0	40.0	24.0	41.0
37	34.08013842408586	35.0	33.0	40.0	24.0	41.0
38	34.08659163448145	35.0	33.0	40.0	24.0	41.0
39	34.06028527206187	35.0	33.0	40.0	24.0	41.0
40	33.852374303220905	35.0	33.0	40.0	23.0	41.0
41	33.83420933281939	35.0	33.0	39.0	23.0	41.0
42	33.74853922086674	35.0	33.0	39.0	23.0	41.0
43	33.637481716329326	35.0	33.0	39.0	23.0	41.0
44	33.5739987696015	35.0	33.0	39.0	23.0	41.0
45	33.54218964984697	35.0	33.0	39.0	23.0	41.0
46	33.54068696398745	35.0	33.0	39.0	23.0	41.0
47	33.47517207284708	35.0	33.0	39.0	23.0	41.0
48	33.432475713243974	35.0	33.0	39.0	23.0	41.0
49	33.41538202341842	35.0	33.0	39.0	23.0	41.0
50	33.2600846303146	35.0	32.0	39.0	23.0	40.0
51	33.09670710984838	35.0	32.0	38.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	10.0
14	12.0
15	51.0
16	134.0
17	354.0
18	805.0
19	1545.0
20	2632.0
21	4083.0
22	6205.0
23	8671.0
24	12245.0
25	16698.0
26	21753.0
27	25566.0
28	28250.0
29	31640.0
30	37117.0
31	44874.0
32	55404.0
33	71492.0
34	125984.0
35	200505.0
36	74525.0
37	89825.0
38	120872.0
39	193845.0
40	132.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.06440021476666	22.007115209035856	27.7964549887724	15.13202958742509
2	30.76004761625181	25.82620068088858	30.179905363124977	13.23384633973464
3	30.411434707618685	24.259016753330627	31.589332802372983	13.740215736677703
4	26.256669976659868	27.821641569430298	30.433643145293388	15.48804530861645
5	23.184502764993034	32.56709968865643	30.050653957654212	14.19774358869633
6	21.799496098207243	41.25366205224684	26.648168144251034	10.298673705294883
7	86.04637904612633	4.4687460911873345	7.614515979438901	1.8703588832474354
8	87.39632871550992	3.7635218327662097	6.920013035757286	1.9201364159665903
9	83.67705357849407	4.9984300931988574	8.594580290309379	2.729936037997701
10	55.393204218071546	20.35688363714646	13.792460873582934	10.45745127119906
11	49.57927348627374	18.965154876198596	18.735072058296723	12.72049957923094
12	45.432932645467396	18.950689610280207	23.092946140709596	12.523431603542798
13	21.275938561761155	41.8573741798407	22.815553394274648	14.051133864123502
14	14.180640538992826	44.806671721000754	26.425658318506436	14.587029421499981
15	13.696564669523983	22.838102191147428	49.9566467471446	13.508686392183991
16	15.272257577033924	16.51601517661664	49.8498590487471	18.36186819760234
17	14.71849316175826	17.600825030696146	29.513652232883974	38.16702957466162
18	20.79594700266927	21.450627920175556	37.33178810257405	20.421636974581126
19	29.68349147272574	23.314094529661876	25.85062145335079	21.151792544261586
20	33.35205308922772	21.020754253000902	25.796163981658044	19.831028676113334
21	21.41625164116951	27.747187994850364	29.052295339886953	21.784265024093177
22	21.738997250748575	23.83110015154493	25.58973612802271	28.84016646968378
23	18.315749526262543	30.955243616350515	25.008998246299228	25.720008611087714
24	19.218211939970846	22.46991862862472	40.97363152202677	17.338237909377664
25	16.85058826832898	24.41251875166457	38.01633553971184	20.720557440294616
26	16.34175126719984	34.19282539828408	28.18795315636357	21.277470178152512
27	17.740627571307378	34.78768818672786	28.856418621392088	18.61526562057267
28	15.117649411306221	28.404166336943693	39.36177544972087	17.11640880202922
29	15.812492714185916	23.0015596960252	39.50132272093354	21.684624868855344
30	18.071456711840842	31.905441407589503	31.48271528357452	18.54038659699514
31	29.509567922507017	27.381131677315658	24.439407128312865	18.669893271864463
32	31.168053204949842	24.983130947245176	26.91994496391767	16.92887088388731
33	28.861098560365683	27.32233462584739	24.43183413615559	19.384732677631337
34	19.570058260985732	27.691454176164815	27.458903754076864	25.27958380877259
35	19.89391003795856	24.960241791174315	32.102254113879084	23.04359405698804
36	31.632813689927662	25.123529116453046	25.038779676131206	18.20487751748808
37	19.779294078005222	33.70211252445268	29.078077549141483	17.440515848400608
38	20.751530127319867	33.398256850367034	23.78770435378977	22.062508668523325
39	20.535827485536863	32.98540114309637	26.346099355955303	20.132672015411465
40	25.43904209307292	25.938178857056794	25.195259817448346	23.427519232421936
41	17.293055225832582	24.58661248148233	27.09548522032727	31.024847072357815
42	21.54822592022491	24.883405702207824	24.714417360361256	28.853951017206008
43	22.729953055957605	24.919909226201874	26.044711286055737	26.305426431784785
44	18.785189950213958	29.578320480519118	29.126919094065922	22.509570475201006
45	16.133366348175546	39.648613163902525	22.262554787194667	21.95546570072726
46	22.343219917139553	34.37389649166248	24.796614106697504	18.48626948450047
47	21.639357095510746	28.599532516641435	25.750896208313446	24.010214179534373
48	22.584874947776136	24.171714619023184	31.924076073684365	21.319334359516315
49	21.139199253932638	23.601272603041622	33.16272828529589	22.096799857729856
50	19.92828631696461	33.3032966341028	27.21682327444268	19.55159377448991
51	18.24793295604516	34.92485294355398	23.91048893449702	22.916725165903838
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1173.0
1	1925.5
2	2678.0
3	5946.0
4	9214.0
5	7011.0
6	4808.0
7	4971.0
8	5134.0
9	5675.0
10	6216.0
11	6357.0
12	6498.0
13	6608.0
14	6718.0
15	6193.0
16	5668.0
17	5650.5
18	5633.0
19	5405.0
20	5177.0
21	4904.5
22	4632.0
23	5095.0
24	5558.0
25	5964.0
26	6689.5
27	7009.0
28	9737.5
29	12466.0
30	14350.0
31	16234.0
32	18676.0
33	21118.0
34	23440.0
35	25762.0
36	28674.5
37	31587.0
38	36887.0
39	42187.0
40	60966.5
41	79746.0
42	103190.0
43	126634.0
44	126415.5
45	126197.0
46	124308.0
47	122419.0
48	108967.5
49	95516.0
50	87512.0
51	79508.0
52	69814.0
53	60120.0
54	53583.5
55	47047.0
56	44382.5
57	41718.0
58	40100.0
59	38482.0
60	35603.0
61	32724.0
62	30130.5
63	27537.0
64	24010.5
65	20484.0
66	16969.5
67	13455.0
68	11653.0
69	9851.0
70	8534.5
71	7218.0
72	6279.0
73	5340.0
74	5010.5
75	3478.5
76	2276.0
77	1731.5
78	1187.0
79	1010.0
80	833.0
81	505.5
82	178.0
83	172.0
84	166.0
85	105.0
86	44.0
87	30.5
88	17.0
89	11.5
90	6.0
91	5.5
92	5.0
93	2.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1175229.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.066698541927355
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.68458814104044	13.770195427827039
2	7.236394111666239	2.4700271366877176
3	2.570499246848582	1.3160980724464817
4	1.1896697264724774	0.8121493834465177
5	0.6981285840614299	0.5957375043839507
6	0.4447250786608069	0.4553993310923352
7	0.35135982978674757	0.419758660629933
8	0.2474827769875409	0.33789711593323174
9	0.22392150159981672	0.3439440688373799
>10	3.2140448108069477	14.05488364977487
>50	1.5605609106992875	19.448881772718135
>100	1.5716002483311717	44.50641053121602
>500	0.005017880741798122	0.5393522677036723
>1k	0.002007152296719249	0.9292650773027229
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4990	0.4245980996044175	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3383	0.2878587917759007	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1450	0.12338020930388886	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03420609940700919	0.0
2	0.0	0.0	0.0	0.11997661732309192	0.0
3	0.0	0.0	0.0	0.16439349267249193	0.0
4	0.0	0.0	0.0	0.26684161129447964	0.0
5	0.0	0.0	0.0	0.44459420249159953	0.0
6	0.0	0.0	0.0	0.6196239201040818	0.0
7	0.0	0.0	0.0	0.7230080265207887	0.0
8	0.0	0.0	0.0	0.9539417424178607	0.0
9	0.0	0.0	0.0	1.0225241208309188	0.0
10	0.0	0.0	0.0	1.1636030084349518	0.0
11	0.0	0.0	0.0	1.3272306929117643	0.0
12	0.0	0.0	0.0	1.468735029513397	0.0
13	0.0	0.0	0.0	1.5199590888243908	0.0
14	0.0	0.0	0.0	1.5385086651197342	0.0
15	0.0	0.0	0.0	1.5745867401161817	0.0
16	0.0	0.0	0.0	1.6509122902855529	0.0
17	0.0	0.0	0.0	1.7437452615617892	0.0
18	0.0	0.0	0.0	1.8744431936243915	0.0
19	0.0	0.0	0.0	1.9379201840662543	0.0
20	0.0	0.0	0.0	1.9985892111239596	0.0
21	8.508979951992336E-5	0.0	0.0	2.0864018842285206	0.0
22	8.508979951992336E-5	0.0	0.0	2.1764268921205994	0.0
23	8.508979951992336E-5	0.0	0.0	2.2882348886897788	0.0
24	8.508979951992336E-5	0.0	0.0	2.371112353422184	0.0
25	8.508979951992336E-5	0.0	0.0	2.436886768451085	0.0
26	8.508979951992336E-5	0.0	0.0	2.502235734482386	0.0
27	1.701795990398467E-4	0.0	0.0	2.571243561893044	0.0
28	2.5526939855977007E-4	0.0	0.0	2.6392303117094627	0.0
29	3.403591980796934E-4	0.0	0.0	2.7203208906519496	0.0
30	3.403591980796934E-4	0.0	0.0	2.8248962542619354	0.0
31	3.403591980796934E-4	0.0	0.0	2.924281140101206	0.0
32	3.403591980796934E-4	0.0	0.0	3.010647286613928	0.0
33	3.403591980796934E-4	0.0	0.0	3.099140678114648	0.0
34	3.403591980796934E-4	0.0	0.0	3.1945263433764826	0.0
35	3.403591980796934E-4	0.0	0.0	3.3131415239072557	0.0
36	3.403591980796934E-4	0.0	0.0	3.412781679145086	0.0
37	3.403591980796934E-4	0.0	0.0	3.5107200383925177	0.0
38	3.403591980796934E-4	0.0	0.0	3.598958160494678	0.0
39	3.403591980796934E-4	0.0	0.0	3.695705262548831	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATACG	30	2.1655433E-6	45.000004	1
TCGTTGA	240	0.0	45.000004	24
GACGTAT	30	2.1655433E-6	45.000004	26
CCGGATA	30	2.1655433E-6	45.000004	19
ACGATAT	30	2.1655433E-6	45.000004	36
CCGACGG	30	2.1655433E-6	45.000004	2
ATACGTA	45	3.8562575E-10	45.000004	44
TGGGACG	60	0.0	45.000004	6
GTACGTA	45	3.8562575E-10	45.000004	23
TACGTAT	45	3.8562575E-10	45.000004	45
CGAAGCA	30	2.1655433E-6	45.000004	40
CTATGCG	25	3.8908605E-5	45.0	1
CCTTAGT	25	3.8908605E-5	45.0	15
ACGTTAG	50	2.1827873E-11	45.0	1
CGTTGTC	20	7.033352E-4	45.0	24
CACGCTA	40	6.8157533E-9	45.0	16
CGCGACA	20	7.033352E-4	45.0	43
ATCGTAC	25	3.8908605E-5	45.0	41
CACCTCG	20	7.033352E-4	45.0	25
CGTTAAT	25	3.8908605E-5	45.0	22
>>END_MODULE