FastQCFastQC Report
Thu 26 May 2016
SRR1545522_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545522_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1570982
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81230.5170651223247624No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC53520.3406786328551186No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15900.10121058038857224No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAACG700.045.0000041
GACGTAT453.8562575E-1045.00000414
CACGATA351.2123019E-745.00000414
TCATCGA2800.045.00000416
ATAACGG1250.045.0000042
GGTACGA207.0340786E-445.018
CACGGAT207.0340786E-445.039
ATTACGT253.8914615E-545.028
CAACCTA207.0340786E-445.037
CGACATA207.0340786E-445.041
TCTAGCG502.1827873E-1145.01
ACCTCAT253.8914615E-545.019
CGTTACG253.8914615E-545.024
CCGTAAA253.8914615E-545.039
TCGTGCA207.0340786E-445.018
GTTCGAC207.0340786E-445.030
CACTACG207.0340786E-445.01
GAACGTA406.8175723E-945.09
CAAGCTA253.8914615E-545.032
TACTCGA207.0340786E-445.040