##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545522_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1570982
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.361417890211346	31.0	31.0	33.0	30.0	34.0
2	31.709693682040914	31.0	31.0	34.0	30.0	34.0
3	31.770130402512567	31.0	31.0	34.0	30.0	34.0
4	35.47654778985373	37.0	35.0	37.0	33.0	37.0
5	35.329286395388365	37.0	35.0	37.0	33.0	37.0
6	35.4053973883851	37.0	35.0	37.0	33.0	37.0
7	35.86787563447576	37.0	35.0	37.0	35.0	37.0
8	35.89864619709201	37.0	35.0	37.0	35.0	37.0
9	37.59513030703089	39.0	37.0	39.0	35.0	39.0
10	36.99611262255073	39.0	37.0	39.0	33.0	39.0
11	36.56897532880708	39.0	35.0	39.0	32.0	39.0
12	35.4595908800992	37.0	35.0	39.0	31.0	39.0
13	34.933706433300955	37.0	34.0	39.0	29.0	39.0
14	35.82868358771775	37.0	35.0	40.0	30.0	41.0
15	36.16557541715946	37.0	35.0	40.0	31.0	41.0
16	36.40688817567611	37.0	35.0	40.0	32.0	41.0
17	36.33736032621634	37.0	35.0	40.0	31.0	41.0
18	36.29119748030213	37.0	35.0	40.0	31.0	41.0
19	36.2188153651665	37.0	35.0	40.0	31.0	41.0
20	36.02385323320063	36.0	35.0	40.0	31.0	41.0
21	35.807869218106894	36.0	34.0	40.0	30.0	41.0
22	35.72608788643027	35.0	34.0	40.0	30.0	41.0
23	35.721174399197444	35.0	34.0	40.0	30.0	41.0
24	35.64120658288892	35.0	34.0	40.0	30.0	41.0
25	35.53361082431243	35.0	34.0	40.0	30.0	41.0
26	35.42244150474035	35.0	34.0	40.0	30.0	41.0
27	35.33522726549381	35.0	34.0	40.0	30.0	41.0
28	35.36214673369905	36.0	34.0	40.0	30.0	41.0
29	35.38397002639114	36.0	34.0	40.0	30.0	41.0
30	35.28596890352659	36.0	34.0	40.0	29.0	41.0
31	35.06065887451288	35.0	34.0	40.0	29.0	41.0
32	34.84992062289702	35.0	34.0	40.0	28.0	41.0
33	34.64452870879488	35.0	34.0	40.0	27.0	41.0
34	34.49147985145597	35.0	34.0	40.0	26.0	41.0
35	34.318416124436816	35.0	33.0	40.0	25.0	41.0
36	34.07865526148613	35.0	33.0	40.0	23.0	41.0
37	33.9927185671128	35.0	33.0	40.0	23.0	41.0
38	33.99738953087941	35.0	33.0	40.0	24.0	41.0
39	33.9451916062692	35.0	33.0	40.0	23.0	41.0
40	33.73976404567334	35.0	33.0	40.0	23.0	41.0
41	33.74336179536112	35.0	33.0	39.0	23.0	41.0
42	33.65949959961349	35.0	33.0	39.0	23.0	41.0
43	33.550589376581016	35.0	33.0	39.0	23.0	41.0
44	33.49175547523778	35.0	33.0	39.0	23.0	41.0
45	33.41884375505257	35.0	33.0	39.0	23.0	41.0
46	33.40695310321824	35.0	33.0	39.0	23.0	41.0
47	33.34688557857442	35.0	33.0	39.0	23.0	41.0
48	33.32979308483483	35.0	33.0	39.0	23.0	41.0
49	33.28850807965973	35.0	33.0	39.0	23.0	41.0
50	33.12786651915808	35.0	32.0	38.0	23.0	40.0
51	32.95547752934152	35.0	32.0	38.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	1.0
12	3.0
13	4.0
14	14.0
15	68.0
16	188.0
17	456.0
18	1004.0
19	2026.0
20	3458.0
21	5546.0
22	8265.0
23	11916.0
24	16675.0
25	22568.0
26	29057.0
27	34260.0
28	37489.0
29	41754.0
30	49032.0
31	59034.0
32	73350.0
33	95833.0
34	170446.0
35	274814.0
36	100059.0
37	116444.0
38	159300.0
39	257738.0
40	178.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.132449639779445	23.229419560504194	28.755771867532538	15.882358932183818
2	31.92620921181783	26.16949143911261	28.53107164817929	13.373227700890272
3	28.997595134762843	24.802703022695358	32.08992846512563	14.109773377416163
4	26.42601888500314	28.756153794250984	30.73599824822945	14.081829072516424
5	23.242086796666033	32.83271227805283	29.933697521677523	13.991503403603605
6	20.932830548026647	40.986529444640354	27.667917264488075	10.41272274284492
7	85.9363761010629	4.626596612819243	7.773227191654647	1.6638000944632085
8	86.67304908649494	3.8908784441833197	7.4054317617897585	2.030640707531977
9	83.3903252869861	5.093947607292764	8.743384710964225	2.77234239475691
10	55.21915591649045	20.400552011417066	14.13033376575925	10.249958306333236
11	50.241504994964934	19.341278257803083	18.09161403504305	12.325602712188937
12	44.20827227810376	19.385008867065313	23.406760866769957	12.99995798806097
13	21.802541340384547	42.87846709892284	21.902096904993183	13.41689465569943
14	14.95994225268017	44.86996031781395	27.100819742046696	13.069277687459183
15	14.247266996057242	21.99044928586069	50.05868940573476	13.703594312347308
16	15.04301131394249	16.674793218509187	49.344422787784964	18.93777267976336
17	14.880246877430805	17.074925110535958	29.889839603509145	38.15498840852409
18	20.791772280013394	21.516223610455114	37.33117247683296	20.360831632698527
19	30.318234072701024	23.16716550539726	26.176238811138514	20.338361610763204
20	32.45645080592903	21.96428730564704	25.048727483828586	20.530534404595343
21	20.953072664104365	28.198349821958494	29.759475283612414	21.089102230324727
22	21.74385193464979	23.359529262588623	25.431609019072148	29.46500978368944
23	18.457818103581072	30.69640517841707	24.593470835439234	26.252305882562627
24	18.190914981839384	23.051123437442314	42.454210169180804	16.303751411537498
25	17.358951280154706	23.640818290725164	38.178540556161686	20.821689872958444
26	16.660661929926633	35.28277217689318	27.152889084661698	20.903676808518494
27	16.649713363997805	35.59480630586474	28.33705287520799	19.418427454929464
28	14.002642932891657	29.215420673184035	39.724898184702305	17.057038209222004
29	15.936401562844132	23.365894707896082	38.72679636049299	21.970907368766795
30	17.997087172227307	31.301568063797042	32.2487463255467	18.452598438428957
31	29.843562816123924	27.043912660998025	25.17718217013308	17.93534235274497
32	31.754342188516482	25.270945179511923	26.50176768416188	16.472944947809715
33	29.61383389497779	26.905846152279274	24.68570613794429	18.79461381479864
34	19.568270037467013	26.648936779670297	28.332087827868175	25.45070535499452
35	20.16910442003791	25.31499406103953	32.8082689680722	21.70763255085036
36	32.0464524736757	25.129759602592515	25.58959937160324	17.23418855212854
37	20.75956312675766	33.71706359461789	28.01979908108432	17.503574197540136
38	21.103679100078804	34.28460669823079	23.718158451210773	20.893555750479635
39	21.196105365943087	32.750725342492785	26.12824335352028	19.92492593804385
40	25.1988883386315	25.510158614166173	26.346005237488402	22.944947809713927
41	17.531518502439873	24.69767317512231	27.31482601328341	30.4559823091544
42	22.17301025727857	24.824345536740715	24.302888257153807	28.699755948826912
43	21.59369107984687	25.52066159892348	26.828060410622147	26.057586910607505
44	18.833252067814907	29.602312438971296	29.333436029184295	22.230999464029505
45	17.14328999313805	39.04284072000825	23.139412163856747	20.67445712299695
46	22.174283346340058	33.56053729450751	25.546377997965603	18.718801361186824
47	22.170909660327105	27.999047729382003	26.24288502350759	23.5871575867833
48	23.14068525291824	23.889516238887524	31.945496511099424	21.02430199709481
49	22.67543485539618	23.253671907125607	33.103816593697445	20.967076643780768
50	19.815503933208657	34.48696420455486	26.37452243246581	19.32300942977068
51	18.7373884614846	34.82102277429022	24.158965538752195	22.28262322547298
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2344.0
1	3331.5
2	4319.0
3	9398.5
4	14478.0
5	10792.0
6	7106.0
7	7439.5
8	7773.0
9	8293.0
10	8813.0
11	9249.0
12	9685.0
13	9601.0
14	9517.0
15	8960.0
16	8403.0
17	8287.5
18	8172.0
19	7612.5
20	7053.0
21	6744.5
22	6436.0
23	7720.5
24	9005.0
25	9358.0
26	9182.0
27	8653.0
28	11962.5
29	15272.0
30	18249.5
31	21227.0
32	24318.5
33	27410.0
34	32209.0
35	37008.0
36	36381.0
37	35754.0
38	46358.5
39	56963.0
40	81890.5
41	106818.0
42	135112.5
43	163407.0
44	166043.5
45	168680.0
46	163085.5
47	157491.0
48	143899.0
49	130307.0
50	119852.5
51	109398.0
52	96469.0
53	83540.0
54	77495.5
55	71451.0
56	63612.0
57	55773.0
58	52137.5
59	48502.0
60	44201.5
61	39901.0
62	37235.5
63	34570.0
64	29438.5
65	24307.0
66	21908.0
67	19509.0
68	16051.0
69	12593.0
70	11743.0
71	10893.0
72	9296.0
73	7699.0
74	6035.0
75	3817.0
76	3263.0
77	2425.5
78	1588.0
79	1329.5
80	1071.0
81	743.5
82	416.0
83	304.0
84	192.0
85	131.0
86	70.0
87	65.5
88	61.0
89	34.0
90	7.0
91	3.5
92	0.0
93	1.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1570982.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.712809942363815
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.28333669451406	13.584729576565802
2	7.744296217594698	2.5885790164405433
3	2.655058448608379	1.3312046171237748
4	1.3269143579965192	0.8870586989995811
5	0.7355496324149313	0.6146550604863158
6	0.462923118157957	0.46420476550202183
7	0.3170979444230956	0.37097183747802087
8	0.23527380556603492	0.31456691174734397
9	0.2104922520513973	0.31661253025876285
>10	2.242027453819793	9.035953902046655
>50	1.081820346122128	13.204807313822151
>100	1.6814250261030326	53.870641099917606
>500	0.021099332976424086	2.151422452204982
>1k	0.001918121179674917	0.4006508196396749
>5k	7.672484718699669E-4	0.8639413977667818
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8123	0.5170651223247624	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5352	0.3406786328551186	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1590	0.10121058038857224	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.023106566466070268	0.0
2	0.0	0.0	0.0	0.11502359670575474	0.0
3	0.0	0.0	0.0	0.1740312747058846	0.0
4	0.0	0.0	0.0	0.30961525975472665	0.0
5	0.0	0.0	0.0	0.5243217299752638	0.0
6	0.0	0.0	0.0	0.7797033957104537	0.0
7	0.0	0.0	0.0	0.9143962184162517	0.0
8	0.0	0.0	0.0	1.2310134680091815	0.0
9	0.0	0.0	0.0	1.3499836408055599	0.0
10	0.0	0.0	0.0	1.5541234718157177	0.0
11	0.0	0.0	0.0	1.8088049385670872	0.0
12	0.0	0.0	0.0	2.0214744662892383	0.0
13	0.0	0.0	0.0	2.1045435275515567	0.0
14	0.0	0.0	0.0	2.136625371901142	0.0
15	0.0	0.0	0.0	2.180101363351076	0.0
16	0.0	0.0	0.0	2.2809936714742753	0.0
17	0.0	0.0	0.0	2.3953807236492843	0.0
18	0.0	0.0	0.0	2.5600547937532068	0.0
19	0.0	0.0	0.0	2.6360582107242476	0.0
20	0.0	0.0	0.0	2.714544151365197	0.0
21	0.0	0.0	0.0	2.8223111404204504	0.0
22	0.0	0.0	0.0	2.9256223177604834	0.0
23	0.0	0.0	0.0	3.0545225852364952	0.0
24	0.0	0.0	0.0	3.152996024142861	0.0
25	0.0	0.0	0.0	3.2281719332239325	0.0
26	0.0	0.0	0.0	3.3013745542596924	0.0
27	0.0	0.0	0.0	3.370121363580232	0.0
28	0.0	0.0	0.0	3.448925576486554	0.0
29	0.0	0.0	0.0	3.5400150988362693	0.0
30	0.0	0.0	0.0	3.6551023499950985	0.0
31	0.0	0.0	0.0	3.762869339050352	0.0
32	0.0	0.0	0.0	3.854531751477738	0.0
33	0.0	0.0	0.0	3.9488676509342566	0.0
34	0.0	0.0	0.0	4.044222021639968	0.0
35	0.0	0.0	0.0	4.184134509497881	0.0
36	0.0	0.0	0.0	4.2874456868379145	0.0
37	0.0	0.0	0.0	4.39718596393848	0.0
38	0.0	0.0	0.0	4.501388303621557	0.0
39	0.0	0.0	0.0	4.602726192916278	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTAACG	70	0.0	45.000004	1
GACGTAT	45	3.8562575E-10	45.000004	14
CACGATA	35	1.2123019E-7	45.000004	14
TCATCGA	280	0.0	45.000004	16
ATAACGG	125	0.0	45.000004	2
GGTACGA	20	7.0340786E-4	45.0	18
CACGGAT	20	7.0340786E-4	45.0	39
ATTACGT	25	3.8914615E-5	45.0	28
CAACCTA	20	7.0340786E-4	45.0	37
CGACATA	20	7.0340786E-4	45.0	41
TCTAGCG	50	2.1827873E-11	45.0	1
ACCTCAT	25	3.8914615E-5	45.0	19
CGTTACG	25	3.8914615E-5	45.0	24
CCGTAAA	25	3.8914615E-5	45.0	39
TCGTGCA	20	7.0340786E-4	45.0	18
GTTCGAC	20	7.0340786E-4	45.0	30
CACTACG	20	7.0340786E-4	45.0	1
GAACGTA	40	6.8175723E-9	45.0	9
CAAGCTA	25	3.8914615E-5	45.0	32
TACTCGA	20	7.0340786E-4	45.0	40
>>END_MODULE