FastQCFastQC Report
Thu 26 May 2016
SRR1545521_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545521_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2267536
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104350.46019115021768126No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC61850.27276303441268407No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGGC207.034739E-445.00000429
TAAGTCG207.034739E-445.00000425
TAACGCG207.034739E-445.0000041
TCAATCG207.034739E-445.00000425
GTCGTAC406.8193913E-945.0000049
ACGTATG207.034739E-445.00000436
CCGAATA207.034739E-445.00000411
CGACTTA406.8193913E-945.00000420
AACCGTA253.8920105E-545.041
CGCTAAG253.8920105E-545.017
TCGTAAT253.8920105E-545.014
CGTGTAA351.2126293E-745.031
ACCGTAT253.8920105E-545.042
GTACGAT453.8562575E-1045.018
CGTATTA302.16644E-644.99999625
CGCAATA302.16644E-644.99999629
TCGGTAA600.044.99999612
CGTTTTT73750.042.9254231
GCGTAAG1950.042.6923071
TAGCCGT2500.042.30000344
Produced by FastQC (version 0.11.5)