##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1545521_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 2267536 Sequences flagged as poor quality 0 Sequence length 51 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.306325015347056 31.0 31.0 33.0 30.0 34.0 2 31.641779005934193 31.0 31.0 34.0 30.0 34.0 3 31.74419105143204 31.0 31.0 34.0 30.0 34.0 4 35.44620989479329 37.0 35.0 37.0 33.0 37.0 5 35.30380465844864 37.0 35.0 37.0 33.0 37.0 6 35.38797311266502 37.0 35.0 37.0 33.0 37.0 7 35.856615727379854 37.0 35.0 37.0 35.0 37.0 8 35.898840856330395 37.0 35.0 37.0 35.0 37.0 9 37.666508933044504 39.0 37.0 39.0 35.0 39.0 10 36.91135840842218 39.0 37.0 39.0 32.0 39.0 11 36.54916790736729 39.0 35.0 39.0 32.0 39.0 12 35.5234999576633 37.0 35.0 39.0 31.0 39.0 13 35.01258193916215 37.0 34.0 39.0 29.0 39.0 14 35.82481953980003 38.0 34.0 40.0 27.0 41.0 15 36.16972123044574 38.0 35.0 40.0 30.0 41.0 16 36.41553298382032 38.0 35.0 40.0 31.0 41.0 17 36.36421604772758 37.0 35.0 40.0 31.0 41.0 18 36.36482110978613 37.0 35.0 40.0 31.0 41.0 19 36.28100502042746 37.0 35.0 40.0 31.0 41.0 20 36.098721255142145 37.0 34.0 40.0 31.0 41.0 21 35.90246593659373 36.0 34.0 40.0 30.0 41.0 22 35.88726264985429 36.0 34.0 40.0 30.0 41.0 23 35.89371105905265 36.0 34.0 40.0 30.0 41.0 24 35.83031360913344 36.0 34.0 40.0 30.0 41.0 25 35.73545469619887 36.0 34.0 40.0 30.0 41.0 26 35.62402449178315 36.0 34.0 40.0 30.0 41.0 27 35.600135124646314 36.0 34.0 40.0 30.0 41.0 28 35.617039376662596 36.0 34.0 40.0 30.0 41.0 29 35.579914497498606 36.0 34.0 40.0 30.0 41.0 30 35.47548043338672 36.0 34.0 40.0 30.0 41.0 31 35.2661183769519 36.0 34.0 40.0 29.0 41.0 32 35.11674963484593 35.0 34.0 40.0 29.0 41.0 33 34.95122282512825 35.0 34.0 40.0 27.0 41.0 34 34.787657175012875 35.0 34.0 40.0 27.0 41.0 35 34.6600794871614 35.0 33.0 40.0 26.0 41.0 36 34.45324616676427 35.0 33.0 40.0 24.0 41.0 37 34.394817546446895 35.0 33.0 40.0 25.0 41.0 38 34.36308706895944 35.0 33.0 40.0 25.0 41.0 39 34.30818209721918 35.0 33.0 40.0 24.0 41.0 40 34.155914613924544 35.0 33.0 40.0 23.0 41.0 41 34.08037843721114 35.0 33.0 40.0 23.0 41.0 42 34.028864811848635 35.0 33.0 40.0 23.0 41.0 43 33.94118285222373 35.0 33.0 40.0 23.0 41.0 44 33.92375644752718 35.0 33.0 40.0 23.0 41.0 45 33.85503559811178 35.0 33.0 39.0 23.0 41.0 46 33.88797355367235 35.0 33.0 39.0 23.0 41.0 47 33.81484395396589 35.0 33.0 39.0 23.0 41.0 48 33.78011021655224 35.0 33.0 39.0 23.0 41.0 49 33.733424298445534 35.0 33.0 39.0 24.0 41.0 50 33.57761552628051 35.0 33.0 39.0 24.0 41.0 51 33.406642717028525 35.0 32.0 39.0 23.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 1.0 13 4.0 14 17.0 15 64.0 16 239.0 17 649.0 18 1397.0 19 2710.0 20 4543.0 21 7599.0 22 11211.0 23 15918.0 24 22385.0 25 30675.0 26 39294.0 27 46276.0 28 51454.0 29 58309.0 30 69700.0 31 84582.0 32 103973.0 33 135594.0 34 231856.0 35 358042.0 36 147223.0 37 181035.0 38 252367.0 39 410203.0 40 216.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 32.729006287000516 23.456650743361955 29.24751801073941 14.566824958898117 2 29.96067096619414 25.100417369338345 31.36139845188786 13.577513212579644 3 28.487794681098777 25.79416600221562 31.82838111500765 13.889658201677946 4 25.04784929544669 27.744212219783943 31.978014902519742 15.229923582249633 5 21.92763422499136 32.66104705724628 30.925374503425747 14.485944214336618 6 19.10258536137905 41.97057951891392 27.980503947897628 10.946331171809401 7 89.14085597758977 3.7352438946945052 5.879597942436195 1.2443021852795282 8 90.7833877830385 2.5275894186464956 5.2473257315429604 1.4416970667720381 9 88.10678198714376 3.218118698005236 6.614051551993 2.0610477628580095 10 52.208564715179826 19.242869793467445 14.78640250915531 13.762162982197415 11 47.95919447364893 21.312914105884097 17.63680929431771 13.091082126149264 12 43.30987468335674 20.277428891977898 21.236796240500702 15.175900184164663 13 23.13555330543815 39.3005006315225 21.735355028541996 15.828591034497357 14 16.551225647575166 41.87232308549898 27.329092018825722 14.24735924810014 15 16.183293231066674 23.062390189174504 46.19860500560961 14.555711574149207 16 18.427579540082277 16.792721262198263 44.248338284375635 20.531360913343825 17 17.997553291325914 17.11747906097191 28.762365845569814 36.12260180213236 18 22.604756881478398 22.069638585671846 35.20045547237177 20.12514906047798 19 30.526042364928273 25.08987729412014 24.030445382124032 20.353634958827556 20 32.32976234996931 24.09249511363877 24.06462345030024 19.513119086091688 21 21.08120885401599 28.18354372323085 29.23084793361605 21.504399489137107 22 21.623559670055954 22.978907501358304 26.460175273953755 28.937357554631987 23 18.95731754644689 30.12556360736941 24.624790962524962 26.29232788365874 24 19.64533308401719 24.186738380338834 39.18799966130637 16.979928874337606 25 19.95633145405409 23.70348254669386 35.33619752894772 21.00398847030433 26 17.0753628608322 33.582840581141824 26.340970992301777 23.000825565724202 27 16.328957952597005 34.169336231045506 27.195202193041258 22.306503623316235 28 15.43481558837434 28.810038738083982 36.07281207442792 19.68233359911375 29 16.951086994870202 24.730809124970893 36.02544788704567 22.29265599311323 30 19.54553312494267 30.4281828381115 31.343096647638667 18.68318738930716 31 29.466257647067124 27.076879926051888 24.348411667995567 19.108450758885414 32 31.30252397315853 25.006747412168977 25.28140677810628 18.40932183656621 33 28.27911001192484 27.185764636151312 23.698940171181405 20.836185180742444 34 18.638733850311528 28.35650679856902 27.22285335271414 25.781905998405318 35 19.442381510150224 25.957779722130102 31.625517742606952 22.974321025112722 36 31.11765369987511 26.280111980581566 23.608974675595007 18.99325964394832 37 20.158489214724707 33.06095250527445 27.47634436674875 19.304213913252095 38 22.32030715278611 32.122400702789285 24.17871204690907 21.37858009751554 39 19.747779087079547 31.040168711764665 25.317040170475796 23.89501203068 40 25.57719039521313 23.633847489080658 26.769497816131693 24.019464299574516 41 18.54034511469719 22.693134750672094 28.372779969094207 30.39374016553651 42 22.284100450885898 25.08537901934082 24.766310215141015 27.86421031463227 43 20.903659302432242 24.389954558604583 27.859756140586082 26.846629998377093 44 20.79980207590971 27.359477423952693 28.714119643524956 23.12660085661264 45 17.4806485983023 36.84223756535729 22.90446546383387 22.772648372506545 46 21.689534366819313 31.889284227460994 25.459132732622546 20.96204867309714 47 20.477337515258853 29.298939465569674 26.03425039337854 24.18947262579293 48 21.376771967457188 26.515080686701335 30.254558251776377 21.8535890940651 49 21.99788669286838 24.326625905829058 31.97113518815137 21.70435221315119 50 21.234635304577303 32.23741541479386 25.759458725241846 20.768490555386993 51 17.980883214202553 35.38761898377798 24.024050775820097 22.607447026199363 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2304.0 1 3015.5 2 3727.0 3 10115.5 4 16504.0 5 11956.0 6 7408.0 7 7908.0 8 8408.0 9 9017.0 10 9626.0 11 9900.0 12 10174.0 13 10049.0 14 9924.0 15 9513.0 16 9102.0 17 9279.5 18 9457.0 19 8313.5 20 7170.0 21 6888.5 22 6607.0 23 7309.0 24 8011.0 25 7862.5 26 9115.5 27 10517.0 28 13579.0 29 16641.0 30 20864.0 31 25087.0 32 29469.0 33 33851.0 34 38266.5 35 42682.0 36 48795.0 37 54908.0 38 73211.5 39 91515.0 40 120687.5 41 149860.0 42 179231.0 43 208602.0 44 218825.0 45 229048.0 46 226924.5 47 224801.0 48 211856.5 49 198912.0 50 189499.5 51 180087.0 52 163654.5 53 147222.0 54 136426.0 55 125630.0 56 115017.5 57 104405.0 58 97580.5 59 90756.0 60 78212.0 61 65668.0 62 55902.5 63 46137.0 64 40605.0 65 35073.0 66 28746.5 67 22420.0 68 17937.0 69 13454.0 70 12390.0 71 11326.0 72 10296.5 73 9267.0 74 7747.0 75 4734.0 76 3241.0 77 2584.5 78 1928.0 79 1567.0 80 1206.0 81 894.5 82 583.0 83 405.5 84 228.0 85 142.0 86 56.0 87 38.0 88 20.0 89 20.0 90 20.0 91 18.0 92 16.0 93 10.5 94 5.0 95 2.5 96 0.0 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 2267536.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 15.639352819541848 #Duplication Level Percentage of deduplicated Percentage of total 1 79.85155370848861 12.488266216356488 2 7.752597780160865 2.424912239038654 3 2.86210736073961 1.342845204660435 4 1.394399706939935 0.872300359531976 5 0.8886392217634894 0.694887115922115 6 0.5787273047636858 0.5430552303301083 7 0.45263756681402023 0.4955271024748386 8 0.3342477573122642 0.4181934884597679 9 0.259933302074876 0.36586697586277817 >10 2.893785025493297 10.45917963607456 >50 1.0223467034205065 11.633621922029572 >100 1.682876173586138 54.65401504944941 >500 0.0224535074670568 2.238656549084145 >1k 0.0031264377485775295 0.6299069149389713 >5k 2.842216135070481E-4 0.2749258534258371 >10k+ 2.842216135070481E-4 0.4638401423603251 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10435 0.46019115021768126 No Hit GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC 6185 0.27276303441268407 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.02610763401330784 0.0 2 0.0 0.0 0.0 0.09344945350371504 0.0 3 0.0 0.0 0.0 0.1358743587753403 0.0 4 0.0 0.0 0.0 0.2401284918960493 0.0 5 0.0 0.0 0.0 0.4006992612245186 0.0 6 0.0 0.0 0.0 0.609383930398459 0.0 7 0.0 0.0 0.0 0.7256334629306878 0.0 8 0.0 0.0 0.0 1.030986939126876 0.0 9 0.0 0.0 0.0 1.1309192004007875 0.0 10 0.0 0.0 0.0 1.3028679588769483 0.0 11 0.0 0.0 0.0 1.4864152101664538 0.0 12 0.0 0.0 0.0 1.644736842105263 0.0 13 4.4100733130587564E-5 0.0 0.0 1.7055076523592128 0.0 14 4.4100733130587564E-5 0.0 0.0 1.7268524071944171 0.0 15 4.4100733130587564E-5 0.0 0.0 1.7598838563092274 0.0 16 4.4100733130587564E-5 0.0 0.0 1.8360017216926214 0.0 17 4.4100733130587564E-5 0.0 0.0 1.9289219663987693 0.0 18 4.4100733130587564E-5 0.0 0.0 2.0669572610975084 0.0 19 4.4100733130587564E-5 0.0 0.0 2.1307269212043383 0.0 20 4.4100733130587564E-5 0.0 0.0 2.2018614037439757 0.0 21 4.4100733130587564E-5 0.0 0.0 2.2971630880391753 0.0 22 4.4100733130587564E-5 0.0 0.0 2.4012408182273624 0.0 23 8.820146626117513E-5 0.0 0.0 2.516961141962024 0.0 24 8.820146626117513E-5 0.0 0.0 2.61120440866209 0.0 25 8.820146626117513E-5 0.0 0.0 2.683044502931817 0.0 26 8.820146626117513E-5 0.0 0.0 2.7617643115699155 0.0 27 8.820146626117513E-5 0.0 0.0 2.8493483675672624 0.0 28 8.820146626117513E-5 0.0 0.0 2.936447315500173 0.0 29 8.820146626117513E-5 0.0 0.0 3.048595479851257 0.0 30 8.820146626117513E-5 0.0 0.0 3.171327820153682 0.0 31 8.820146626117513E-5 0.0 0.0 3.283828790369811 0.0 32 8.820146626117513E-5 0.0 0.0 3.3807622017908425 0.0 33 8.820146626117513E-5 0.0 0.0 3.4850604356446824 0.0 34 1.323021993917627E-4 0.0 0.0 3.5983552190571615 0.0 35 1.323021993917627E-4 0.0 0.0 3.7477685029035923 0.0 36 1.323021993917627E-4 0.0 0.0 3.860489976785374 0.0 37 1.323021993917627E-4 0.0 0.0 3.981105481897531 0.0 38 1.323021993917627E-4 0.0 0.0 4.1532747440393445 0.0 39 1.7640293252235026E-4 0.0 0.0 4.500347513777069 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGACGGC 20 7.034739E-4 45.000004 29 TAAGTCG 20 7.034739E-4 45.000004 25 TAACGCG 20 7.034739E-4 45.000004 1 TCAATCG 20 7.034739E-4 45.000004 25 GTCGTAC 40 6.8193913E-9 45.000004 9 ACGTATG 20 7.034739E-4 45.000004 36 CCGAATA 20 7.034739E-4 45.000004 11 CGACTTA 40 6.8193913E-9 45.000004 20 AACCGTA 25 3.8920105E-5 45.0 41 CGCTAAG 25 3.8920105E-5 45.0 17 TCGTAAT 25 3.8920105E-5 45.0 14 CGTGTAA 35 1.2126293E-7 45.0 31 ACCGTAT 25 3.8920105E-5 45.0 42 GTACGAT 45 3.8562575E-10 45.0 18 CGTATTA 30 2.16644E-6 44.999996 25 CGCAATA 30 2.16644E-6 44.999996 29 TCGGTAA 60 0.0 44.999996 12 CGTTTTT 7375 0.0 42.925423 1 GCGTAAG 195 0.0 42.692307 1 TAGCCGT 250 0.0 42.300003 44 >>END_MODULE