FastQCFastQC Report
Thu 26 May 2016
SRR1545520_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545520_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2847052
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163690.5749455928448093No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC82200.2887197002372981No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA42650.1498040780428317No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCTA207.035044E-445.00000412
ACATTCG207.035044E-445.00000422
CCGTCTA207.035044E-445.00000435
CTCGAAT207.035044E-445.00000437
ATCGATC207.035044E-445.00000418
TCGTTAA351.2127748E-745.035
CGTAAGT302.1666365E-644.99999620
GCGTAAG1900.043.815791
CGTAAGG4850.043.1443332
CGTTTTT124650.042.2202151
TGTCACG800.042.1875041
CGACGGT3700.041.95945728
CGGTCTA3750.041.431
CGTTAGG3050.041.3114782
AGCTACG2700.040.8333329
TATTGCG1550.040.645161
ACGTAAG1400.040.178571
AAGGGAC19650.040.0763365
GTCGACG900.040.01
ATCGCAA451.9299478E-840.010